Cyclin D and cdk4 are required for normal development beyond the blastula stage in sea urchin embryos

Jennifer C. Moore, Jan L. Sumerel, Bradley J. Schnackenberg, Jason A. Nichols, Athula Wikramanayake, Gary M. Wessel, William F. Marzluff

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

cdk4 mRNA and protein are constitutively expressed in sea urchin eggs and throughout embryonic development. In contrast, cyclin D mRNA is barely detectable in eggs and early embryos, when the cell cycles consist of alternating S and M phases. Cyclin D mRNA increases dramatically in embryos at the early blastula stage and remains at a constant level throughout embryogenesis. An increase in cdk4 kinase activity occurs concomitantly with the increase in cyclin D mRNA. Ectopic expression of cyclin D mRNA in eggs arrests development before the 16-cell stage and causes eventual embryonic death, suggesting that activation of cyclin D/cdk4 in cleavage cell cycles is lethal to the embryo. In contrast, blocking cyclin D or cdk4 expression with morpholino antisense oligonucleotides results in normal development of early gastrula-stage embryos but abnormal, asymmetric larvae. These results suggest that in sea urchins, cyclin D and cdk4 are required for normal development and perhaps the patterning of the developing embryo, but may not be directly involved in regulating entry into the cell cycle.

Original languageEnglish
Pages (from-to)4863-4875
Number of pages13
JournalMolecular and Cellular Biology
Volume22
Issue number13
DOIs
StatePublished - Jun 22 2002
Externally publishedYes

Fingerprint

Cyclin D
Blastula
Sea Urchins
Embryonic Structures
Messenger RNA
Eggs
Cell Cycle
Embryonic Development
Cyclin-Dependent Kinase 4
Gastrula
Morpholinos
Antisense Oligonucleotides
S Phase
Cell Division
Larva
Phosphotransferases

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Moore, J. C., Sumerel, J. L., Schnackenberg, B. J., Nichols, J. A., Wikramanayake, A., Wessel, G. M., & Marzluff, W. F. (2002). Cyclin D and cdk4 are required for normal development beyond the blastula stage in sea urchin embryos. Molecular and Cellular Biology, 22(13), 4863-4875. https://doi.org/10.1128/MCB.22.13.4863-4875.2002

Cyclin D and cdk4 are required for normal development beyond the blastula stage in sea urchin embryos. / Moore, Jennifer C.; Sumerel, Jan L.; Schnackenberg, Bradley J.; Nichols, Jason A.; Wikramanayake, Athula; Wessel, Gary M.; Marzluff, William F.

In: Molecular and Cellular Biology, Vol. 22, No. 13, 22.06.2002, p. 4863-4875.

Research output: Contribution to journalArticle

Moore, Jennifer C. ; Sumerel, Jan L. ; Schnackenberg, Bradley J. ; Nichols, Jason A. ; Wikramanayake, Athula ; Wessel, Gary M. ; Marzluff, William F. / Cyclin D and cdk4 are required for normal development beyond the blastula stage in sea urchin embryos. In: Molecular and Cellular Biology. 2002 ; Vol. 22, No. 13. pp. 4863-4875.
@article{a9d6b1f792e54d0a83c6fdd63ce80064,
title = "Cyclin D and cdk4 are required for normal development beyond the blastula stage in sea urchin embryos",
abstract = "cdk4 mRNA and protein are constitutively expressed in sea urchin eggs and throughout embryonic development. In contrast, cyclin D mRNA is barely detectable in eggs and early embryos, when the cell cycles consist of alternating S and M phases. Cyclin D mRNA increases dramatically in embryos at the early blastula stage and remains at a constant level throughout embryogenesis. An increase in cdk4 kinase activity occurs concomitantly with the increase in cyclin D mRNA. Ectopic expression of cyclin D mRNA in eggs arrests development before the 16-cell stage and causes eventual embryonic death, suggesting that activation of cyclin D/cdk4 in cleavage cell cycles is lethal to the embryo. In contrast, blocking cyclin D or cdk4 expression with morpholino antisense oligonucleotides results in normal development of early gastrula-stage embryos but abnormal, asymmetric larvae. These results suggest that in sea urchins, cyclin D and cdk4 are required for normal development and perhaps the patterning of the developing embryo, but may not be directly involved in regulating entry into the cell cycle.",
author = "Moore, {Jennifer C.} and Sumerel, {Jan L.} and Schnackenberg, {Bradley J.} and Nichols, {Jason A.} and Athula Wikramanayake and Wessel, {Gary M.} and Marzluff, {William F.}",
year = "2002",
month = "6",
day = "22",
doi = "10.1128/MCB.22.13.4863-4875.2002",
language = "English",
volume = "22",
pages = "4863--4875",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "13",

}

TY - JOUR

T1 - Cyclin D and cdk4 are required for normal development beyond the blastula stage in sea urchin embryos

AU - Moore, Jennifer C.

AU - Sumerel, Jan L.

AU - Schnackenberg, Bradley J.

AU - Nichols, Jason A.

AU - Wikramanayake, Athula

AU - Wessel, Gary M.

AU - Marzluff, William F.

PY - 2002/6/22

Y1 - 2002/6/22

N2 - cdk4 mRNA and protein are constitutively expressed in sea urchin eggs and throughout embryonic development. In contrast, cyclin D mRNA is barely detectable in eggs and early embryos, when the cell cycles consist of alternating S and M phases. Cyclin D mRNA increases dramatically in embryos at the early blastula stage and remains at a constant level throughout embryogenesis. An increase in cdk4 kinase activity occurs concomitantly with the increase in cyclin D mRNA. Ectopic expression of cyclin D mRNA in eggs arrests development before the 16-cell stage and causes eventual embryonic death, suggesting that activation of cyclin D/cdk4 in cleavage cell cycles is lethal to the embryo. In contrast, blocking cyclin D or cdk4 expression with morpholino antisense oligonucleotides results in normal development of early gastrula-stage embryos but abnormal, asymmetric larvae. These results suggest that in sea urchins, cyclin D and cdk4 are required for normal development and perhaps the patterning of the developing embryo, but may not be directly involved in regulating entry into the cell cycle.

AB - cdk4 mRNA and protein are constitutively expressed in sea urchin eggs and throughout embryonic development. In contrast, cyclin D mRNA is barely detectable in eggs and early embryos, when the cell cycles consist of alternating S and M phases. Cyclin D mRNA increases dramatically in embryos at the early blastula stage and remains at a constant level throughout embryogenesis. An increase in cdk4 kinase activity occurs concomitantly with the increase in cyclin D mRNA. Ectopic expression of cyclin D mRNA in eggs arrests development before the 16-cell stage and causes eventual embryonic death, suggesting that activation of cyclin D/cdk4 in cleavage cell cycles is lethal to the embryo. In contrast, blocking cyclin D or cdk4 expression with morpholino antisense oligonucleotides results in normal development of early gastrula-stage embryos but abnormal, asymmetric larvae. These results suggest that in sea urchins, cyclin D and cdk4 are required for normal development and perhaps the patterning of the developing embryo, but may not be directly involved in regulating entry into the cell cycle.

UR - http://www.scopus.com/inward/record.url?scp=0036269990&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036269990&partnerID=8YFLogxK

U2 - 10.1128/MCB.22.13.4863-4875.2002

DO - 10.1128/MCB.22.13.4863-4875.2002

M3 - Article

C2 - 12052892

AN - SCOPUS:0036269990

VL - 22

SP - 4863

EP - 4875

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 13

ER -