Cyclin C regulates human hematopoietic stem/progenitor sell quiescence

Yasuhiko Miyata, Yan Liu, Vladimir Jankovic, Goro Sashida, Jennifer May Lee, Jae Hung Shieh, Tomoki Naoe, Malcolm Moore, Stephen D. Nimer

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Hematopoietic stem cells (HSCs) can remain quiescent or they can enter the cell cycle, and either self-renew or differentiate. Although cyclin C and cyclin dependent kinase (cdk3) are essential for the transition from the G0 to the G1 phase of the cell cycle in human fibroblasts, the role of cyclin C in hematopoietic stem/progenitor cells (HSPCs) is not clear. We have identified an important role of cyclin C (CCNC) in regulating human HSPC quiescence, as knocking down CCNC expression in human cord blood CD34+ cells resulted in a significant increase in quiescent cells that maintain CD34 expression. CCNC knockdown also promotes in vitro HSPC expansion and enhances their engraftment potential in sublethally irradiated immunodeficient mice. Our studies establish cyclin C as a critical regulator of the G0/G 1 transition of human HSPCs and suggest that modulating cyclin C levels may be useful for HSC expansion and more efficient engraftment.

Original languageEnglish (US)
Pages (from-to)308-317
Number of pages10
JournalSTEM CELLS
Volume28
Issue number2
DOIs
StatePublished - Feb 1 2010
Externally publishedYes

Keywords

  • Cyclin C
  • Engraftment
  • Hematopoietic stem/progenitor cell
  • Human cord blood CD34 cells
  • Quiescence

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

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  • Cite this

    Miyata, Y., Liu, Y., Jankovic, V., Sashida, G., Lee, J. M., Shieh, J. H., Naoe, T., Moore, M., & Nimer, S. D. (2010). Cyclin C regulates human hematopoietic stem/progenitor sell quiescence. STEM CELLS, 28(2), 308-317. https://doi.org/10.1002/stem.270