Cyclin A is required at two points in the human cell cycle

Michele Pagano, Rainer Pepperkok, Fulvia Verde, Wilhelm Ansorge, Giulio Draetta

Research output: Contribution to journalArticle

1018 Citations (Scopus)

Abstract

Cyclins play a fundamental role in regulating cell cycle events in all eukaryotic cells. The human cyclin A gene was identified as the site of integration of hepatitis B virus in a hepatocarcinoma cell line; in addition, cyclin A is associated with the E2F transcription factor in a complex which is dissociated by the E1A oncogene product. Such findings suggest that cyclin A is a target for oncogenic signals. We have now found that DNA synthesis and entry into mitosis are inhibited in human cells micro-injected with anti-cyclin A antibodies at distinct times. Cyclin A binds both cdk2 and cdc2, giving two distinct cyclin A kinase activities, one appearing in S phase, the other in G2. These results suggest that cyclin A defines novel control points of the human cell cycle.

Original languageEnglish
Pages (from-to)961-971
Number of pages11
JournalEMBO Journal
Volume11
Issue number3
StatePublished - Dec 1 1992
Externally publishedYes

Fingerprint

Cyclin A
Cell Cycle
Cells
E2F Transcription Factors
Cyclins
Oncogene Proteins
Eukaryotic Cells
S Phase
Viruses
Mitosis
Hepatitis B virus
Phosphotransferases
Genes
Cell Line
Antibodies
DNA

Keywords

  • cdc2
  • Cdk2
  • Cyclin A
  • DNA replication
  • Mitosis
  • S phase

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Pagano, M., Pepperkok, R., Verde, F., Ansorge, W., & Draetta, G. (1992). Cyclin A is required at two points in the human cell cycle. EMBO Journal, 11(3), 961-971.

Cyclin A is required at two points in the human cell cycle. / Pagano, Michele; Pepperkok, Rainer; Verde, Fulvia; Ansorge, Wilhelm; Draetta, Giulio.

In: EMBO Journal, Vol. 11, No. 3, 01.12.1992, p. 961-971.

Research output: Contribution to journalArticle

Pagano, M, Pepperkok, R, Verde, F, Ansorge, W & Draetta, G 1992, 'Cyclin A is required at two points in the human cell cycle', EMBO Journal, vol. 11, no. 3, pp. 961-971.
Pagano M, Pepperkok R, Verde F, Ansorge W, Draetta G. Cyclin A is required at two points in the human cell cycle. EMBO Journal. 1992 Dec 1;11(3):961-971.
Pagano, Michele ; Pepperkok, Rainer ; Verde, Fulvia ; Ansorge, Wilhelm ; Draetta, Giulio. / Cyclin A is required at two points in the human cell cycle. In: EMBO Journal. 1992 ; Vol. 11, No. 3. pp. 961-971.
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