Cyclin A is required at two points in the human cell cycle

M. Pagano, R. Pepperkok, F. Verde, W. Ansorge, G. Draetta

Research output: Contribution to journalArticle

1044 Scopus citations

Abstract

Cyclins play a fundamental role in regulating cell cycle events in all eukaryotic cells. The human cyclin A gene was identified as the site of integration of hepatitis B virus in a hepatocarcinoma cell line; in addition, cyclin A is associated with the E2F transcription factor in a complex which is dissociated by the E1A oncogene product. Such findings suggest that cyclin A is a target for oncogenic signals. We have now found that DNA synthesis and entry into mitosis are inhibited in human cells micro-injected with anti-cyclin A antibodies at distinct times. Cyclin A binds both cdk2 and cdc2, giving two distinct cyclin A kinase activities, one appearing in S phase, the other in G2. These results suggest that cyclin A defines novel control points of the human cell cycle.

Original languageEnglish (US)
Pages (from-to)961-971
Number of pages11
JournalEMBO Journal
Volume11
Issue number3
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

Keywords

  • cdc2
  • Cdk2
  • Cyclin A
  • DNA replication
  • Mitosis
  • S phase

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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