Cyclic AMP-dependent protein phosphorylation in the rat anterior pituitary

S. T. Cain, J. C. Pryor, Charles Nemeroff

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The activation of cyclic adenosine 3'5'-monophosphate (cAMP)-dependent protein kinases has been implicated as an integral mechanism in stimulus-secretion coupling in the anterior pituitary. Therefore, we have investigated phosphorylation of endogenous protein substrates both in the presence and absence of cAMP in cell-free extracts of the rodent anterior pituitary. Specific phosphoprotein substrates in the rat anterior pituitary, which are phosphorylated by a cAMP-dependent protein kinase in vitro, were identified. Cyclic AMP potentiated the phosphorylation of proteins with apparent molecular weights of 85,000, 77,000, 63,000, 53,000, 39,000, and 33,000 as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins with apparent molecular weights of 124,000, 93,000, 48,000, and 43,000 were phosphorylated only in the presence of cAMP and not in the basal condition. The results highlight endogenous protein substrates that may potentially be involved in cAMP-dependent stimulus-secretion coupling in the anterior pituitary.

Original languageEnglish
Pages (from-to)241-246
Number of pages6
JournalSynapse
Volume5
Issue number3
DOIs
StatePublished - May 16 1990
Externally publishedYes

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Cyclic AMP
Adenosine
Phosphorylation
Proteins
Protein Kinases
Molecular Weight
Phosphoproteins
Cell Extracts
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Rodentia

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology
  • Pharmacology

Cite this

Cyclic AMP-dependent protein phosphorylation in the rat anterior pituitary. / Cain, S. T.; Pryor, J. C.; Nemeroff, Charles.

In: Synapse, Vol. 5, No. 3, 16.05.1990, p. 241-246.

Research output: Contribution to journalArticle

Cain, S. T. ; Pryor, J. C. ; Nemeroff, Charles. / Cyclic AMP-dependent protein phosphorylation in the rat anterior pituitary. In: Synapse. 1990 ; Vol. 5, No. 3. pp. 241-246.
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