Cybrid technology

Sandra R. Bacman; Nadee Nissanka; Carlos T. Moraes

doi:10.1016/bs.mcb.2019.11.025

Cybrid technology

Sandra R. Bacman, Nadee Nissanka, Carlos T. Moraes

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The study of the mitochondrial DNA (mtDNA) has been hampered by the lack of methods to genetically manipulate the mitochondrial genome in living animal cells. This limitation has been partially alleviated by the ability to transfer mitochondria (and their mtDNAs) from one cell into another, as long as they are from the same species. This is done by isolating mtDNA-containing cytoplasts and fusing these to cells lacking mtDNA. This transmitochondrial cytoplasmic hybrid (cybrid) technology has helped the field understand the mechanism of several pathogenic mutations. In this chapter, we describe procedures to obtain transmitochondrial cybrids.

Original language	English (US)
Title of host publication	Methods in Cell Biology
Publisher	Academic Press Inc.
DOIs	https://doi.org/10.1016/bs.mcb.2019.11.025
State	Accepted/In press - Jan 1 2019
Externally published	Yes

Publication series

Name	Methods in Cell Biology
ISSN (Print)	0091-679X

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Keywords

Mitochondrial diseases
mtDNA
Rho zero cells
Transmitochondrial cybrids

ASJC Scopus subject areas

Cell Biology

Cite this

Bacman, S. R., Nissanka, N., & Moraes, C. T. (Accepted/In press). Cybrid technology. In Methods in Cell Biology (Methods in Cell Biology). Academic Press Inc.. https://doi.org/10.1016/bs.mcb.2019.11.025

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Access to Document

10.1016/bs.mcb.2019.11.025