Cybrid technology

Sandra R. Bacman, Nadee Nissanka, Carlos T. Moraes

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations


The study of the mitochondrial DNA (mtDNA) has been hampered by the lack of methods to genetically manipulate the mitochondrial genome in living animal cells. This limitation has been partially alleviated by the ability to transfer mitochondria (and their mtDNAs) from one cell into another, as long as they are from the same species. This is done by isolating mtDNA-containing cytoplasts and fusing these to cells lacking mtDNA. This transmitochondrial cytoplasmic hybrid (cybrid) technology has helped the field understand the mechanism of several pathogenic mutations. In this chapter, we describe procedures to obtain transmitochondrial cybrids.

Original languageEnglish (US)
Title of host publicationMitochondria, 3rd Edition
EditorsLiza A. Pon, Eric A. Schon
PublisherAcademic Press Inc.
Number of pages25
ISBN (Print)9780128202289
StatePublished - 2020

Publication series

NameMethods in Cell Biology
ISSN (Print)0091-679X


  • Mitochondrial diseases
  • Rho zero cells
  • Transmitochondrial cybrids
  • mtDNA

ASJC Scopus subject areas

  • Cell Biology


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