@inbook{258415793000448ba176c3ef95479d1d,
title = "Cybrid technology",
abstract = "The study of the mitochondrial DNA (mtDNA) has been hampered by the lack of methods to genetically manipulate the mitochondrial genome in living animal cells. This limitation has been partially alleviated by the ability to transfer mitochondria (and their mtDNAs) from one cell into another, as long as they are from the same species. This is done by isolating mtDNA-containing cytoplasts and fusing these to cells lacking mtDNA. This transmitochondrial cytoplasmic hybrid (cybrid) technology has helped the field understand the mechanism of several pathogenic mutations. In this chapter, we describe procedures to obtain transmitochondrial cybrids.",
keywords = "Mitochondrial diseases, Rho zero cells, Transmitochondrial cybrids, mtDNA",
author = "Bacman, {Sandra R.} and Nadee Nissanka and Moraes, {Carlos T.}",
note = "Funding Information: This work was supported by grants from the National Institutes of Health (5R01EY0108041, R01AG036871, 1R01NS079965, 1R33ES025673), and the Muscular Dystrophy Association. Barrientos et al., 2000 A. Barrientos S. Muller R. Dey J. Wienberg C.T. Moraes Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
doi = "10.1016/bs.mcb.2019.11.025",
language = "English (US)",
isbn = "9780128202289",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "415--439",
editor = "Pon, {Liza A.} and Schon, {Eric A.}",
booktitle = "Mitochondria, 3rd Edition",
}