Cybrid technology

Sandra R. Bacman, Nadee Nissanka, Carlos T. Moraes

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The study of the mitochondrial DNA (mtDNA) has been hampered by the lack of methods to genetically manipulate the mitochondrial genome in living animal cells. This limitation has been partially alleviated by the ability to transfer mitochondria (and their mtDNAs) from one cell into another, as long as they are from the same species. This is done by isolating mtDNA-containing cytoplasts and fusing these to cells lacking mtDNA. This transmitochondrial cytoplasmic hybrid (cybrid) technology has helped the field understand the mechanism of several pathogenic mutations. In this chapter, we describe procedures to obtain transmitochondrial cybrids.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
DOIs
StateAccepted/In press - Jan 1 2019
Externally publishedYes

Publication series

NameMethods in Cell Biology
ISSN (Print)0091-679X

Keywords

  • Mitochondrial diseases
  • mtDNA
  • Rho zero cells
  • Transmitochondrial cybrids

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Bacman, S. R., Nissanka, N., & Moraes, C. T. (Accepted/In press). Cybrid technology. In Methods in Cell Biology (Methods in Cell Biology). Academic Press Inc.. https://doi.org/10.1016/bs.mcb.2019.11.025