CX3CR1+ interstitial dendritic cells form a contiguous network throughout the entire kidney

T. J. Soos, T. N. Sims, L. Barisoni, K. Lin, D. R. Littman, M. L. Dustin, P. J. Nelson

Research output: Contribution to journalArticlepeer-review

232 Scopus citations


Dendritic cells (DCs) interface innate and adaptive immunity in nonlymphoid organs; however, the exact distribution and types of DC within the kidney are not known. We utilized CX3CR1GFP/+ mice to characterize the anatomy and phenotype of tissue-resident CX3CR1+ DCs within normal kidney. Laser-scanning confocal microscopy revealed an extensive, contiguous network of stellate-shaped CX3CR1+ DCs throughout the interstitial and mesangial spaces of the entire kidney. Intravital microscopy of the superficial cortex showed stationary interstitial CX3CR1+ DCs that continually probe the surrounding tissue environment through dendrite extensions. Flow cytometry of renal CX 3CR1+ DCs showed significant coexpression of CD11c and F4/80, high major histocompatibility complex class II and FcR expression, and immature costimulatory but competent phagocytic ability indicative of tissue-resident, immature DCs ready to respond to environment cues. Thus, within the renal parenchyma, there exists little immunological privilege from the surveillance provided by renal CX3CR1+ DCs, a major constituent of the heterogeneous mononuclear phagocyte system populating normal kidney.

Original languageEnglish (US)
Pages (from-to)591-596
Number of pages6
JournalKidney international
Issue number3
StatePublished - Aug 21 2006
Externally publishedYes


  • Adaptive immunity
  • Chemokine
  • Dendritic cells
  • Innate immunity
  • Renal

ASJC Scopus subject areas

  • Nephrology


Dive into the research topics of 'CX<sub>3</sub>CR1<sup>+</sup> interstitial dendritic cells form a contiguous network throughout the entire kidney'. Together they form a unique fingerprint.

Cite this