Abstract
Dendritic cells (DCs) interface innate and adaptive immunity in nonlymphoid organs; however, the exact distribution and types of DC within the kidney are not known. We utilized CX3CR1GFP/+ mice to characterize the anatomy and phenotype of tissue-resident CX3CR1+ DCs within normal kidney. Laser-scanning confocal microscopy revealed an extensive, contiguous network of stellate-shaped CX3CR1+ DCs throughout the interstitial and mesangial spaces of the entire kidney. Intravital microscopy of the superficial cortex showed stationary interstitial CX3CR1+ DCs that continually probe the surrounding tissue environment through dendrite extensions. Flow cytometry of renal CX 3CR1+ DCs showed significant coexpression of CD11c and F4/80, high major histocompatibility complex class II and FcR expression, and immature costimulatory but competent phagocytic ability indicative of tissue-resident, immature DCs ready to respond to environment cues. Thus, within the renal parenchyma, there exists little immunological privilege from the surveillance provided by renal CX3CR1+ DCs, a major constituent of the heterogeneous mononuclear phagocyte system populating normal kidney.
| Original language | English |
|---|---|
| Pages (from-to) | 591-596 |
| Number of pages | 6 |
| Journal | Kidney International |
| Volume | 70 |
| Issue number | 3 |
| DOIs | |
| State | Published - Aug 21 2006 |
| Externally published | Yes |
Fingerprint
Keywords
- Adaptive immunity
- Chemokine
- Dendritic cells
- Innate immunity
- Renal
ASJC Scopus subject areas
- Nephrology
Cite this
CX3CR1+ interstitial dendritic cells form a contiguous network throughout the entire kidney. / Soos, T. J.; Sims, T. N.; Barisoni-Thomas, Laura; Lin, K.; Littman, D. R.; Dustin, M. L.; Nelson, P. J.
In: Kidney International, Vol. 70, No. 3, 21.08.2006, p. 591-596.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CX3CR1+ interstitial dendritic cells form a contiguous network throughout the entire kidney
AU - Soos, T. J.
AU - Sims, T. N.
AU - Barisoni-Thomas, Laura
AU - Lin, K.
AU - Littman, D. R.
AU - Dustin, M. L.
AU - Nelson, P. J.
PY - 2006/8/21
Y1 - 2006/8/21
N2 - Dendritic cells (DCs) interface innate and adaptive immunity in nonlymphoid organs; however, the exact distribution and types of DC within the kidney are not known. We utilized CX3CR1GFP/+ mice to characterize the anatomy and phenotype of tissue-resident CX3CR1+ DCs within normal kidney. Laser-scanning confocal microscopy revealed an extensive, contiguous network of stellate-shaped CX3CR1+ DCs throughout the interstitial and mesangial spaces of the entire kidney. Intravital microscopy of the superficial cortex showed stationary interstitial CX3CR1+ DCs that continually probe the surrounding tissue environment through dendrite extensions. Flow cytometry of renal CX 3CR1+ DCs showed significant coexpression of CD11c and F4/80, high major histocompatibility complex class II and FcR expression, and immature costimulatory but competent phagocytic ability indicative of tissue-resident, immature DCs ready to respond to environment cues. Thus, within the renal parenchyma, there exists little immunological privilege from the surveillance provided by renal CX3CR1+ DCs, a major constituent of the heterogeneous mononuclear phagocyte system populating normal kidney.
AB - Dendritic cells (DCs) interface innate and adaptive immunity in nonlymphoid organs; however, the exact distribution and types of DC within the kidney are not known. We utilized CX3CR1GFP/+ mice to characterize the anatomy and phenotype of tissue-resident CX3CR1+ DCs within normal kidney. Laser-scanning confocal microscopy revealed an extensive, contiguous network of stellate-shaped CX3CR1+ DCs throughout the interstitial and mesangial spaces of the entire kidney. Intravital microscopy of the superficial cortex showed stationary interstitial CX3CR1+ DCs that continually probe the surrounding tissue environment through dendrite extensions. Flow cytometry of renal CX 3CR1+ DCs showed significant coexpression of CD11c and F4/80, high major histocompatibility complex class II and FcR expression, and immature costimulatory but competent phagocytic ability indicative of tissue-resident, immature DCs ready to respond to environment cues. Thus, within the renal parenchyma, there exists little immunological privilege from the surveillance provided by renal CX3CR1+ DCs, a major constituent of the heterogeneous mononuclear phagocyte system populating normal kidney.
KW - Adaptive immunity
KW - Chemokine
KW - Dendritic cells
KW - Innate immunity
KW - Renal
UR - http://www.scopus.com/inward/record.url?scp=33746482680&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33746482680&partnerID=8YFLogxK
U2 - 10.1038/sj.ki.5001567
DO - 10.1038/sj.ki.5001567
M3 - Article
C2 - 16760907
AN - SCOPUS:33746482680
VL - 70
SP - 591
EP - 596
JO - Kidney International
JF - Kidney International
SN - 0085-2538
IS - 3
ER -