CX3CR1+ interstitial dendritic cells form a contiguous network throughout the entire kidney

T. J. Soos, T. N. Sims, L. Barisoni, K. Lin, D. R. Littman, M. L. Dustin, P. J. Nelson

Research output: Contribution to journalArticle

217 Scopus citations

Abstract

Dendritic cells (DCs) interface innate and adaptive immunity in nonlymphoid organs; however, the exact distribution and types of DC within the kidney are not known. We utilized CX3CR1GFP/+ mice to characterize the anatomy and phenotype of tissue-resident CX3CR1+ DCs within normal kidney. Laser-scanning confocal microscopy revealed an extensive, contiguous network of stellate-shaped CX3CR1+ DCs throughout the interstitial and mesangial spaces of the entire kidney. Intravital microscopy of the superficial cortex showed stationary interstitial CX3CR1+ DCs that continually probe the surrounding tissue environment through dendrite extensions. Flow cytometry of renal CX 3CR1+ DCs showed significant coexpression of CD11c and F4/80, high major histocompatibility complex class II and FcR expression, and immature costimulatory but competent phagocytic ability indicative of tissue-resident, immature DCs ready to respond to environment cues. Thus, within the renal parenchyma, there exists little immunological privilege from the surveillance provided by renal CX3CR1+ DCs, a major constituent of the heterogeneous mononuclear phagocyte system populating normal kidney.

Original languageEnglish (US)
Pages (from-to)591-596
Number of pages6
JournalKidney international
Volume70
Issue number3
DOIs
StatePublished - Aug 21 2006
Externally publishedYes

Keywords

  • Adaptive immunity
  • Chemokine
  • Dendritic cells
  • Innate immunity
  • Renal

ASJC Scopus subject areas

  • Nephrology

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    Soos, T. J., Sims, T. N., Barisoni, L., Lin, K., Littman, D. R., Dustin, M. L., & Nelson, P. J. (2006). CX3CR1+ interstitial dendritic cells form a contiguous network throughout the entire kidney. Kidney international, 70(3), 591-596. https://doi.org/10.1038/sj.ki.5001567