Cutting edge: Tumor secreted heat shock-fusion protein elicits CD8 cells for rejection

Koichi Yamazaki, Timmy Nguyen, Eckhard R. Podack

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

The endoplasmic reticulum resident heat shock protein gp96 chaperons peptides, including those derived from tumor Ags, on their way to presentation by MHC class I. Replacement of the endoplasmic reticulum retention signal of gp96 with the Fc portion of murine IgG1 generated a secretory form of gp96, gp96-Ig. Tumor cells secreting gp96-Ig exhibited decreased were rejected after initial growth. Rejection required CD8 T cells during the priming and effector phase. CD4 T cells were not required for rejection in either phase. Carrageenan, a compound known to inactivate macrophages in vivo, did not diminish CD8-mediated tumor rejection. Therefore, immunization with tumors secreting gp96-Ig generates efficient tumor-rejecting CD8 CTL without requirement for CD4 or macrophage help. In contrast, immunization with purified, tumor-derived gp96 or irradiated tumor cells requires both.

Original languageEnglish (US)
Pages (from-to)5178-5182
Number of pages5
JournalJournal of Immunology
Volume163
Issue number10
StatePublished - Nov 15 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Yamazaki, K., Nguyen, T., & Podack, E. R. (1999). Cutting edge: Tumor secreted heat shock-fusion protein elicits CD8 cells for rejection. Journal of Immunology, 163(10), 5178-5182.