TY - JOUR
T1 - Cutting edge
T2 - Tumor secreted heat shock-fusion protein elicits CD8 cells for rejection
AU - Yamazaki, Koichi
AU - Nguyen, Timmy
AU - Podack, Eckhard R.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1999/11/15
Y1 - 1999/11/15
N2 - The endoplasmic reticulum resident heat shock protein gp96 chaperons peptides, including those derived from tumor Ags, on their way to presentation by MHC class I. Replacement of the endoplasmic reticulum retention signal of gp96 with the Fc portion of murine IgG1 generated a secretory form of gp96, gp96-Ig. Tumor cells secreting gp96-Ig exhibited decreased were rejected after initial growth. Rejection required CD8 T cells during the priming and effector phase. CD4 T cells were not required for rejection in either phase. Carrageenan, a compound known to inactivate macrophages in vivo, did not diminish CD8-mediated tumor rejection. Therefore, immunization with tumors secreting gp96-Ig generates efficient tumor-rejecting CD8 CTL without requirement for CD4 or macrophage help. In contrast, immunization with purified, tumor-derived gp96 or irradiated tumor cells requires both.
AB - The endoplasmic reticulum resident heat shock protein gp96 chaperons peptides, including those derived from tumor Ags, on their way to presentation by MHC class I. Replacement of the endoplasmic reticulum retention signal of gp96 with the Fc portion of murine IgG1 generated a secretory form of gp96, gp96-Ig. Tumor cells secreting gp96-Ig exhibited decreased were rejected after initial growth. Rejection required CD8 T cells during the priming and effector phase. CD4 T cells were not required for rejection in either phase. Carrageenan, a compound known to inactivate macrophages in vivo, did not diminish CD8-mediated tumor rejection. Therefore, immunization with tumors secreting gp96-Ig generates efficient tumor-rejecting CD8 CTL without requirement for CD4 or macrophage help. In contrast, immunization with purified, tumor-derived gp96 or irradiated tumor cells requires both.
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M3 - Article
C2 - 10553037
AN - SCOPUS:0033571087
VL - 163
SP - 5178
EP - 5182
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 10
ER -