Cutting edge: Senescent BALB/c mice exhibit decreased expression of λ5 surrogate light chains and reduced development within the pre-B cell compartment

Erin M. Sherwood, Bonnie B. Blomberg, Wei Xu, Cynthia A. Warner, Richard L. Riley

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

Although senescent BALB/c mice (~2 years old) have reduced numbers of small pre-B cells, early pre-B cells (CD43+CD25+B220+) are present in comparable numbers within the bone marrow of both young (3-6-month-old) and senescent BALB/c mice. The transition of CD43+ pre-B cells to the CD43- pre- B cell compartments is dependent on proliferation and clonal maturation dictated by the pre-B cell receptor (μ/λ5/VpreB). In vivo, senescent CD43+B220+ pro-B/early pre-B cells demonstrated reduction of λ5 mRNA, by RT-PCR analysis, and of both surface and cytoplasmic λ5 protein. Decreased λ5 protein expression was also seen among pro-B/pre-B cells derived from senescent bone marrow after stimulation in vitro with IL-7. We propose that diminished expression of the λ5 surrogate light chain results in decreased pre-B cell receptor formation and contributes to reduced recruitment of nascent CD43+ pre-B cells into the CD43- large and small pre-B cell compartments.

Original languageEnglish (US)
Pages (from-to)4472-4475
Number of pages4
JournalJournal of Immunology
Volume161
Issue number9
StatePublished - Nov 1 1998

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Cutting edge: Senescent BALB/c mice exhibit decreased expression of λ5 surrogate light chains and reduced development within the pre-B cell compartment'. Together they form a unique fingerprint.

  • Cite this