Abstract
Although senescent BALB/c mice (~2 years old) have reduced numbers of small pre-B cells, early pre-B cells (CD43+CD25+B220+) are present in comparable numbers within the bone marrow of both young (3-6-month-old) and senescent BALB/c mice. The transition of CD43+ pre-B cells to the CD43- pre- B cell compartments is dependent on proliferation and clonal maturation dictated by the pre-B cell receptor (μ/λ5/VpreB). In vivo, senescent CD43+B220+ pro-B/early pre-B cells demonstrated reduction of λ5 mRNA, by RT-PCR analysis, and of both surface and cytoplasmic λ5 protein. Decreased λ5 protein expression was also seen among pro-B/pre-B cells derived from senescent bone marrow after stimulation in vitro with IL-7. We propose that diminished expression of the λ5 surrogate light chain results in decreased pre-B cell receptor formation and contributes to reduced recruitment of nascent CD43+ pre-B cells into the CD43- large and small pre-B cell compartments.
Original language | English (US) |
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Pages (from-to) | 4472-4475 |
Number of pages | 4 |
Journal | Journal of Immunology |
Volume | 161 |
Issue number | 9 |
State | Published - Nov 1 1998 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology