Cutting edge: DNA sensing via the STING adaptor in myeloid dendritic cells s tolerogenic responses

Lei Huang, Lingqian Li, Henrique Lemos, Phillip R. Chandler, Gabriela Pacholczyk, Babak Baban, Glen N Barber, Yoshihiro Hayakawa, Tracy L. McGaha, Buvana Ravishankar, David H. Munn, Andrew L. Mellor

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Cytosolic DNA sensing via the stimulator of IFN genes (STING) adaptor incites autoimmunity by inducing type I IFN (IFN-αβ). In this study, we show that DNA is also sensed via STING to suppress immunity by inducing IDO. STING gene ablation abolished IFN-αβ and IDO induction by dendritic cells (DCs) after DNA nanoparticle (DNP) treatment. Marginal zone macrophages, some DCs, and myeloid cells ingested DNPs, but CD11b+ DCs were the only cells to express IFN-β, whereas CD11b+ non-DCs were major IL-1β producers. STING ablation also abolished DNP-induced regulatory responses by DCs and regulatory T cells, and hallmark regulatory responses to apoptotic cells were also abrogated. Moreover, systemic cyclic diguanylate monophosphate treatment to activate STING induced selective IFN-β expression by CD11b+ DCs and suppressed Th1 responses to immunization. Thus, previously unrecognized functional diversity among physiologic innate immune cells regarding DNA sensing via STING is pivotal in driving immune responses to DNA.

Original languageEnglish
Pages (from-to)3509-3513
Number of pages5
JournalJournal of Immunology
Volume191
Issue number7
DOIs
StatePublished - Oct 1 2013

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Myeloid Cells
Dendritic Cells
DNA
Genes
Nanoparticles
Regulatory T-Lymphocytes
Autoimmunity
Interleukin-1
Immunity
Immunization
Macrophages
Therapeutics

ASJC Scopus subject areas

  • Immunology

Cite this

Huang, L., Li, L., Lemos, H., Chandler, P. R., Pacholczyk, G., Baban, B., ... Mellor, A. L. (2013). Cutting edge: DNA sensing via the STING adaptor in myeloid dendritic cells s tolerogenic responses. Journal of Immunology, 191(7), 3509-3513. https://doi.org/10.4049/jimmunol.1301419

Cutting edge : DNA sensing via the STING adaptor in myeloid dendritic cells s tolerogenic responses. / Huang, Lei; Li, Lingqian; Lemos, Henrique; Chandler, Phillip R.; Pacholczyk, Gabriela; Baban, Babak; Barber, Glen N; Hayakawa, Yoshihiro; McGaha, Tracy L.; Ravishankar, Buvana; Munn, David H.; Mellor, Andrew L.

In: Journal of Immunology, Vol. 191, No. 7, 01.10.2013, p. 3509-3513.

Research output: Contribution to journalArticle

Huang, L, Li, L, Lemos, H, Chandler, PR, Pacholczyk, G, Baban, B, Barber, GN, Hayakawa, Y, McGaha, TL, Ravishankar, B, Munn, DH & Mellor, AL 2013, 'Cutting edge: DNA sensing via the STING adaptor in myeloid dendritic cells s tolerogenic responses', Journal of Immunology, vol. 191, no. 7, pp. 3509-3513. https://doi.org/10.4049/jimmunol.1301419
Huang, Lei ; Li, Lingqian ; Lemos, Henrique ; Chandler, Phillip R. ; Pacholczyk, Gabriela ; Baban, Babak ; Barber, Glen N ; Hayakawa, Yoshihiro ; McGaha, Tracy L. ; Ravishankar, Buvana ; Munn, David H. ; Mellor, Andrew L. / Cutting edge : DNA sensing via the STING adaptor in myeloid dendritic cells s tolerogenic responses. In: Journal of Immunology. 2013 ; Vol. 191, No. 7. pp. 3509-3513.
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