Cultured gill epithelia as models for the freshwater fish gill

Chris M. Wood, Scott P. Kelly, Bingsheng Zhou, Mary Fletcher, Mike O'Donnell, Brighitta Eletti, Peter Pärt

Research output: Contribution to journalArticle

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Abstract

We review recent progress in the development of models for the freshwater teleost gill based on reconstructed flat epithelia grown on permeable filter supports in primary culture. Methods are available for single-seeded insert (SSI) preparations consisting of pavement cells (PVCs) only from trout and tilapia, and double-seeded insert (DSI) preparations from trout, containing both PVCs (85%) and mitochondria-rich cells (MRCs, 15%), as in the intact gill. While there are some quantitative differences, both SSI and DSI epithelia manifest electrical and passive permeability characteristics typical of intact gills and representative of very tight epithelia. Both preparations withstand apical freshwater exposure, exhibiting large increases in transepithelial resistance (TER), negative transepithelial potential (TEP), and low rates of ion loss, but there is only a small active apical-to-basolateral "influx" of Cl- (and not of Na+). Responses to various hormonal treatments are described (thyroid hormone T3, prolactin, and cortisol). Cortisol has the most marked effects, stimulating Na+,K+-ATPase activity and promoting active Na+ and Cl- influxes in DSI preparations, and raising TER and reducing passive ion effluxes in both epithelia via reductions in paracellular permeability. Experiments using DSI epithelia lacking Na+ uptake demonstrate that both NH3 and NH4+ diffusion occur, but are not large enough to account for normal rates of branchial ammonia excretion, suggesting that Na+-linked carrier-mediated processes are important for ammonia excretion in vivo. Future research goals are suggested.

Original languageEnglish
Pages (from-to)72-83
Number of pages12
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1566
Issue number1-2
DOIs
StatePublished - Nov 13 2002

Fingerprint

Fresh Water
Ammonia
Pavements
Fish
Hydrocortisone
Fishes
Epithelium
Ions
Negative resistance
Mitochondria
Triiodothyronine
Prolactin
Trout
Adenosine Triphosphatases
Permeability
Tilapia
Experiments

Keywords

  • Freshwater fish
  • Gill model
  • Ion transport
  • Mitochondria-rich cell
  • Pavement cell
  • Primary culture

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Wood, C. M., Kelly, S. P., Zhou, B., Fletcher, M., O'Donnell, M., Eletti, B., & Pärt, P. (2002). Cultured gill epithelia as models for the freshwater fish gill. Biochimica et Biophysica Acta - Biomembranes, 1566(1-2), 72-83. https://doi.org/10.1016/S0005-2736(02)00595-3

Cultured gill epithelia as models for the freshwater fish gill. / Wood, Chris M.; Kelly, Scott P.; Zhou, Bingsheng; Fletcher, Mary; O'Donnell, Mike; Eletti, Brighitta; Pärt, Peter.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1566, No. 1-2, 13.11.2002, p. 72-83.

Research output: Contribution to journalArticle

Wood, CM, Kelly, SP, Zhou, B, Fletcher, M, O'Donnell, M, Eletti, B & Pärt, P 2002, 'Cultured gill epithelia as models for the freshwater fish gill', Biochimica et Biophysica Acta - Biomembranes, vol. 1566, no. 1-2, pp. 72-83. https://doi.org/10.1016/S0005-2736(02)00595-3
Wood CM, Kelly SP, Zhou B, Fletcher M, O'Donnell M, Eletti B et al. Cultured gill epithelia as models for the freshwater fish gill. Biochimica et Biophysica Acta - Biomembranes. 2002 Nov 13;1566(1-2):72-83. https://doi.org/10.1016/S0005-2736(02)00595-3
Wood, Chris M. ; Kelly, Scott P. ; Zhou, Bingsheng ; Fletcher, Mary ; O'Donnell, Mike ; Eletti, Brighitta ; Pärt, Peter. / Cultured gill epithelia as models for the freshwater fish gill. In: Biochimica et Biophysica Acta - Biomembranes. 2002 ; Vol. 1566, No. 1-2. pp. 72-83.
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