Culture of preantral follicles in poly(ethylene) glycol-based, three-dimensional hydrogel: A relationship between swelling ratio and follicular developments

Jong Il Ahn, Gil Ah Kim, Hyo Suk Kwon, Ji Yeon Ahn, Jeffrey A. Hubbell, Yong Sang Song, Seung Tae Lee, Jeong Mook Lim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study was undertaken to examine how the softness of poly(ethylene) glycol (PEG)-based hydrogels, creating a three-dimensional (3D) microenvironment, influences the in vitro growth of mouse ovarian follicles. Early secondary, preantral follicles of 2week-old mice were cultured in a crosslinked four-arm PEG hydrogel. The hydrogel swelling ratio, which relates to softness, was modified within the range 25.7-15.5 by increasing the reactive PEG concentration in the precursor solution from 5% to 15% w/v, but it did not influence follicular growth to form the pseudoantrum (60-80%; p=0.76). Significant (p<0.04) model effects, however, were detected in the maturation and developmental competence of the follicle-derived oocytes. A swelling ratio of>21.4 yielded better oocyte maturation than other levels, while the highest competence to develop pronuclear and blastocyst formation was detected at 20.6. In conclusion, gel softness, as reflected in swelling ratio, was one of the essential factors for supporting folliculogenesis in vivo within a hydrogel-based, 3D microenvironment.

Original languageEnglish (US)
Pages (from-to)319-323
Number of pages5
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume9
Issue number3
DOIs
StatePublished - Mar 1 2015
Externally publishedYes

Fingerprint

Ethylene Glycol
Hydrogel
Hydrogels
Polyethylene glycols
Swelling
Ovarian Follicle
Blastocyst
Growth
Mental Competency
Oocytes
Gels

Keywords

  • Mouse
  • PEG-based hydrogel
  • Preantral follicle
  • Swelling ratio
  • Three dimensional culture

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials

Cite this

Culture of preantral follicles in poly(ethylene) glycol-based, three-dimensional hydrogel : A relationship between swelling ratio and follicular developments. / Ahn, Jong Il; Kim, Gil Ah; Kwon, Hyo Suk; Ahn, Ji Yeon; Hubbell, Jeffrey A.; Song, Yong Sang; Lee, Seung Tae; Lim, Jeong Mook.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 9, No. 3, 01.03.2015, p. 319-323.

Research output: Contribution to journalArticle

Ahn, Jong Il ; Kim, Gil Ah ; Kwon, Hyo Suk ; Ahn, Ji Yeon ; Hubbell, Jeffrey A. ; Song, Yong Sang ; Lee, Seung Tae ; Lim, Jeong Mook. / Culture of preantral follicles in poly(ethylene) glycol-based, three-dimensional hydrogel : A relationship between swelling ratio and follicular developments. In: Journal of Tissue Engineering and Regenerative Medicine. 2015 ; Vol. 9, No. 3. pp. 319-323.
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