Culture and expansion of rodent and porcine Schwann cells for preclinical animal studies

Adriana E. Brooks, Gagani Athauda, Mary B Bunge, Aisha Khan

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Cell-based therapies have become a major focus in preclinical research that leads to clinical application of a therapeutic product. Since 1990, scientists at the Miami Project to Cure Paralysis have generated extensive data demonstrating that Schwann cell (SC) transplantation supports spinal cord repair in animals with spinal cord injury. After preclinical efforts in SC transplantation strategies, efficient methods for procuring large, essentially pure populations of SCs from the adult peripheral nerve were developed for rodent and pig studies. This chapter describes a series of simple procedures to obtain and cryopreserve large cultures of highly purified adult nerve-derived SCs without the need for additional purification steps. This protocol permits the derivation of ≥90% pure rodent and porcine SCs within 2–4 weeks of culture.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages111-126
Number of pages16
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1739
ISSN (Print)1064-3745

Keywords

  • Clinical trials
  • Fibroblasts
  • Food and Drug Administration (FDA)
  • Peripheral nerve
  • Preclinical research
  • Regenerative medicine
  • Schwann cells
  • Spinal cord injury

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Brooks, A. E., Athauda, G., Bunge, M. B., & Khan, A. (2018). Culture and expansion of rodent and porcine Schwann cells for preclinical animal studies. In Methods in Molecular Biology (pp. 111-126). (Methods in Molecular Biology; Vol. 1739). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7649-2_7