Cu uptake, metabolism and elimination in fed and starved European eels (Anguilla anguilla) during adaptation to water-borne Cu exposure

Martin Grosell, H. J M Hansen, P. Rosenkilde

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Abstract

64Cu accumulation and total Cu concentrations were measured in gill filaments, plasma, liver and bile of fed and starved European eels (Anguilla anguilla) during 28 days of exposure to 12 and 94 μg Cu l-1. Branchial Cu uptake was found to be 0.03 and 0.13 μg Cu g gill filament-1 h-1 at 12 and 94 μg Cu l-1, respectively, throughout 28 days of Cu exposure. At least during exposure to 94 μg Cu l-1, the basolateral membrane seemed to be the rate limiting step for branchial Cu uptake. The plasma accounted for at least 70% of the 64Cu labelled Cu present in the blood at both Cu concentrations. The plasma Cu concentration was gradually increased from 0.64 to 1.92 and 2.68 μg ml-1 during the first 6 days of Cu exposure; it was partly normalized to 1.07 and 1.45 μg ml-1 after 28 days of exposure to 12 and 94 μg Cu l-1, respectively, despite a constant branchial Cu uptake, indicating an increased turnover of plasma Cu. The hepatic accumulation of 64Cu was similar in fed and starved eels (0.55 μg Cu g liver-1 h-1) at both Cu concentrations during the 28 days of exposure. The hepatic total Cu concentrations were lower in the fed fish compared to the starved fish and increased only marginally from 3 to 28 days of exposure indicating a high turnover of hepatic Cu during acclimation. Hepatic Cu elimination, measured as 64Cu in the bile, was highest in the fed fish at the highest Cu concentration and was stimulated during acclimation at least in the starved fish. Copyright (C) 1998 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)295-305
Number of pages11
JournalComparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology
Volume120
Issue number2
DOIs
StatePublished - Aug 1 1998
Externally publishedYes

Fingerprint

Anguilla
Eels
Fishes
Water
Liver
Acclimatization
Bile
Membranes

Keywords

  • Cu
  • Cu elimination
  • Cu turnover
  • Cu uptake
  • Cu-acclimation
  • European eel (Anguilla anguilla)
  • Hepatobiliary Cu metabolism

ASJC Scopus subject areas

  • Pharmacology
  • Immunology

Cite this

@article{84ae749f2f00468b8a812ac9cd2633eb,
title = "Cu uptake, metabolism and elimination in fed and starved European eels (Anguilla anguilla) during adaptation to water-borne Cu exposure",
abstract = "64Cu accumulation and total Cu concentrations were measured in gill filaments, plasma, liver and bile of fed and starved European eels (Anguilla anguilla) during 28 days of exposure to 12 and 94 μg Cu l-1. Branchial Cu uptake was found to be 0.03 and 0.13 μg Cu g gill filament-1 h-1 at 12 and 94 μg Cu l-1, respectively, throughout 28 days of Cu exposure. At least during exposure to 94 μg Cu l-1, the basolateral membrane seemed to be the rate limiting step for branchial Cu uptake. The plasma accounted for at least 70{\%} of the 64Cu labelled Cu present in the blood at both Cu concentrations. The plasma Cu concentration was gradually increased from 0.64 to 1.92 and 2.68 μg ml-1 during the first 6 days of Cu exposure; it was partly normalized to 1.07 and 1.45 μg ml-1 after 28 days of exposure to 12 and 94 μg Cu l-1, respectively, despite a constant branchial Cu uptake, indicating an increased turnover of plasma Cu. The hepatic accumulation of 64Cu was similar in fed and starved eels (0.55 μg Cu g liver-1 h-1) at both Cu concentrations during the 28 days of exposure. The hepatic total Cu concentrations were lower in the fed fish compared to the starved fish and increased only marginally from 3 to 28 days of exposure indicating a high turnover of hepatic Cu during acclimation. Hepatic Cu elimination, measured as 64Cu in the bile, was highest in the fed fish at the highest Cu concentration and was stimulated during acclimation at least in the starved fish. Copyright (C) 1998 Elsevier Science Inc.",
keywords = "Cu, Cu elimination, Cu turnover, Cu uptake, Cu-acclimation, European eel (Anguilla anguilla), Hepatobiliary Cu metabolism",
author = "Martin Grosell and Hansen, {H. J M} and P. Rosenkilde",
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T1 - Cu uptake, metabolism and elimination in fed and starved European eels (Anguilla anguilla) during adaptation to water-borne Cu exposure

AU - Grosell, Martin

AU - Hansen, H. J M

AU - Rosenkilde, P.

PY - 1998/8/1

Y1 - 1998/8/1

N2 - 64Cu accumulation and total Cu concentrations were measured in gill filaments, plasma, liver and bile of fed and starved European eels (Anguilla anguilla) during 28 days of exposure to 12 and 94 μg Cu l-1. Branchial Cu uptake was found to be 0.03 and 0.13 μg Cu g gill filament-1 h-1 at 12 and 94 μg Cu l-1, respectively, throughout 28 days of Cu exposure. At least during exposure to 94 μg Cu l-1, the basolateral membrane seemed to be the rate limiting step for branchial Cu uptake. The plasma accounted for at least 70% of the 64Cu labelled Cu present in the blood at both Cu concentrations. The plasma Cu concentration was gradually increased from 0.64 to 1.92 and 2.68 μg ml-1 during the first 6 days of Cu exposure; it was partly normalized to 1.07 and 1.45 μg ml-1 after 28 days of exposure to 12 and 94 μg Cu l-1, respectively, despite a constant branchial Cu uptake, indicating an increased turnover of plasma Cu. The hepatic accumulation of 64Cu was similar in fed and starved eels (0.55 μg Cu g liver-1 h-1) at both Cu concentrations during the 28 days of exposure. The hepatic total Cu concentrations were lower in the fed fish compared to the starved fish and increased only marginally from 3 to 28 days of exposure indicating a high turnover of hepatic Cu during acclimation. Hepatic Cu elimination, measured as 64Cu in the bile, was highest in the fed fish at the highest Cu concentration and was stimulated during acclimation at least in the starved fish. Copyright (C) 1998 Elsevier Science Inc.

AB - 64Cu accumulation and total Cu concentrations were measured in gill filaments, plasma, liver and bile of fed and starved European eels (Anguilla anguilla) during 28 days of exposure to 12 and 94 μg Cu l-1. Branchial Cu uptake was found to be 0.03 and 0.13 μg Cu g gill filament-1 h-1 at 12 and 94 μg Cu l-1, respectively, throughout 28 days of Cu exposure. At least during exposure to 94 μg Cu l-1, the basolateral membrane seemed to be the rate limiting step for branchial Cu uptake. The plasma accounted for at least 70% of the 64Cu labelled Cu present in the blood at both Cu concentrations. The plasma Cu concentration was gradually increased from 0.64 to 1.92 and 2.68 μg ml-1 during the first 6 days of Cu exposure; it was partly normalized to 1.07 and 1.45 μg ml-1 after 28 days of exposure to 12 and 94 μg Cu l-1, respectively, despite a constant branchial Cu uptake, indicating an increased turnover of plasma Cu. The hepatic accumulation of 64Cu was similar in fed and starved eels (0.55 μg Cu g liver-1 h-1) at both Cu concentrations during the 28 days of exposure. The hepatic total Cu concentrations were lower in the fed fish compared to the starved fish and increased only marginally from 3 to 28 days of exposure indicating a high turnover of hepatic Cu during acclimation. Hepatic Cu elimination, measured as 64Cu in the bile, was highest in the fed fish at the highest Cu concentration and was stimulated during acclimation at least in the starved fish. Copyright (C) 1998 Elsevier Science Inc.

KW - Cu

KW - Cu elimination

KW - Cu turnover

KW - Cu uptake

KW - Cu-acclimation

KW - European eel (Anguilla anguilla)

KW - Hepatobiliary Cu metabolism

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VL - 120

SP - 295

EP - 305

JO - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology

JF - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology

SN - 1532-0456

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