Crystalline lens MTF measurement during simulated accommodation

David Borja, Gaku Takeuchi, Noel Marysa Ziebarth, Ana Carolina Acosta, Fabrice Manns, Jean-Marie A Parel

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Purpose: To design and test an optical system to measure the optical quality of post mortem lenses during simulated accommodation. Methods: An optical bench top system was designed to measure the point spread function and calculate the modulation transfer function (MTF) of monkey and human ex-vivo crystalline lenses. The system consists of a super luminescent diode emitting at 850nm, collimated into a 3mm beam which is focused by the ex-vivo lens under test. The intensity distribution at the focus (point spread function) is re-imaged and magnified onto a beam profiler CCD camera. The optical quality in terms of spatial frequency response (modulation transfer function) is calculated by Fourier transform of the point spread function. The system was used on ex-vivo lenses with attached zonules, ciliary body and sciera. The sciera was glued to 8 separate PMMA segments and stretched radial by 5mm on an accommodation simulating lens stretching device. The point spread function was measured for each lens in the relaxed and stretched state for 5 human (ages 38-86 years) and 5 cynomolgus monkey (ages 53-67 months) fresh post mortem crystalline lenses. Results: Stretching induced measurable changes in the MTF. The cutoff frequency increased from 54.4±13.6 lp/mm unstretched to 59.5±21.4 lp/mm stretched in the post-presbyopic human and from 51.9±24.7 lp/mm unstretched to 57.7±18.5 lp/mm stretched cynomolgus monkey lenses. Conclusion: The results demonstrate the feasibility of measuring the optical quality of ex-vivo human and cynomolgus monkey lenses during simulated accommodation. Additional experiments are underway to quantify changes in optical quality induced by stretching.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsF. Manns, P.G. Soederberg, A. Ho, B.E. Stuck, M. Belkin
Pages26-32
Number of pages7
Volume5688
DOIs
StatePublished - 2005
EventOphtalmic Technologies XV - San Jose, CA, United States
Duration: Jan 22 2005Jan 25 2005

Other

OtherOphtalmic Technologies XV
CountryUnited States
CitySan Jose, CA
Period1/22/051/25/05

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Optical transfer function
Lenses
Crystalline materials
Stretching
Cutoff frequency
CCD cameras
Optical systems
Frequency response
Fourier transforms
Diodes

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Borja, D., Takeuchi, G., Ziebarth, N. M., Acosta, A. C., Manns, F., & Parel, J-M. A. (2005). Crystalline lens MTF measurement during simulated accommodation. In F. Manns, P. G. Soederberg, A. Ho, B. E. Stuck, & M. Belkin (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 5688, pp. 26-32). [06] https://doi.org/10.1117/12.600618

Crystalline lens MTF measurement during simulated accommodation. / Borja, David; Takeuchi, Gaku; Ziebarth, Noel Marysa; Acosta, Ana Carolina; Manns, Fabrice; Parel, Jean-Marie A.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. ed. / F. Manns; P.G. Soederberg; A. Ho; B.E. Stuck; M. Belkin. Vol. 5688 2005. p. 26-32 06.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Borja, D, Takeuchi, G, Ziebarth, NM, Acosta, AC, Manns, F & Parel, J-MA 2005, Crystalline lens MTF measurement during simulated accommodation. in F Manns, PG Soederberg, A Ho, BE Stuck & M Belkin (eds), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 5688, 06, pp. 26-32, Ophtalmic Technologies XV, San Jose, CA, United States, 1/22/05. https://doi.org/10.1117/12.600618
Borja D, Takeuchi G, Ziebarth NM, Acosta AC, Manns F, Parel J-MA. Crystalline lens MTF measurement during simulated accommodation. In Manns F, Soederberg PG, Ho A, Stuck BE, Belkin M, editors, Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 5688. 2005. p. 26-32. 06 https://doi.org/10.1117/12.600618
Borja, David ; Takeuchi, Gaku ; Ziebarth, Noel Marysa ; Acosta, Ana Carolina ; Manns, Fabrice ; Parel, Jean-Marie A. / Crystalline lens MTF measurement during simulated accommodation. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. editor / F. Manns ; P.G. Soederberg ; A. Ho ; B.E. Stuck ; M. Belkin. Vol. 5688 2005. pp. 26-32
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