Crystal Structure of RNase T, an Exoribonuclease Involved in tRNA Maturation and End Turnover

Yuhong Zuo, Heping Zheng, Yong Wang, Maksymilian Chruszcz, Marcin Cymborowski, Tatiana Skarina, Alexei Savchenko, Arun Malhotra, Wladek Minor

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The 3′ processing of most bacterial precursor tRNAs involves exonucleolytic trimming to yield a mature CCA end. This step is carried out by RNase T, a member of the large DEDD family of exonucleases. We report the crystal structures of RNase T from Escherichia coli and Pseudomonas aeruginosa, which show that this enzyme adopts an opposing dimeric arrangement, with the catalytic DEDD residues from one monomer closely juxtaposed with a large basic patch on the other monomer. This arrangement suggests that RNase T has to be dimeric for substrate specificity, and agrees very well with prior site-directed mutagenesis studies. The dimeric architecture of RNase T is very similar to the arrangement seen in oligoribonuclease, another bacterial DEDD family exoribonuclease. The catalytic residues in these two enzymes are organized very similarly to the catalytic domain of the third DEDD family exoribonuclease in E. coli, RNase D, which is monomeric.

Original languageEnglish
Pages (from-to)417-428
Number of pages12
JournalStructure
Volume15
Issue number4
DOIs
StatePublished - Apr 1 2007

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Exoribonucleases
Transfer RNA
Ribonuclease III
Escherichia coli
Exonucleases
RNA Precursors
Enzymes
Substrate Specificity
Site-Directed Mutagenesis
Pseudomonas aeruginosa
Catalytic Domain
exoribonuclease T

Keywords

  • RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Zuo, Y., Zheng, H., Wang, Y., Chruszcz, M., Cymborowski, M., Skarina, T., ... Minor, W. (2007). Crystal Structure of RNase T, an Exoribonuclease Involved in tRNA Maturation and End Turnover. Structure, 15(4), 417-428. https://doi.org/10.1016/j.str.2007.02.004

Crystal Structure of RNase T, an Exoribonuclease Involved in tRNA Maturation and End Turnover. / Zuo, Yuhong; Zheng, Heping; Wang, Yong; Chruszcz, Maksymilian; Cymborowski, Marcin; Skarina, Tatiana; Savchenko, Alexei; Malhotra, Arun; Minor, Wladek.

In: Structure, Vol. 15, No. 4, 01.04.2007, p. 417-428.

Research output: Contribution to journalArticle

Zuo, Y, Zheng, H, Wang, Y, Chruszcz, M, Cymborowski, M, Skarina, T, Savchenko, A, Malhotra, A & Minor, W 2007, 'Crystal Structure of RNase T, an Exoribonuclease Involved in tRNA Maturation and End Turnover', Structure, vol. 15, no. 4, pp. 417-428. https://doi.org/10.1016/j.str.2007.02.004
Zuo Y, Zheng H, Wang Y, Chruszcz M, Cymborowski M, Skarina T et al. Crystal Structure of RNase T, an Exoribonuclease Involved in tRNA Maturation and End Turnover. Structure. 2007 Apr 1;15(4):417-428. https://doi.org/10.1016/j.str.2007.02.004
Zuo, Yuhong ; Zheng, Heping ; Wang, Yong ; Chruszcz, Maksymilian ; Cymborowski, Marcin ; Skarina, Tatiana ; Savchenko, Alexei ; Malhotra, Arun ; Minor, Wladek. / Crystal Structure of RNase T, an Exoribonuclease Involved in tRNA Maturation and End Turnover. In: Structure. 2007 ; Vol. 15, No. 4. pp. 417-428.
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