Crystal structure of a σ70 subunit fragment from E. coli RNA polymerase

Arun Malhotra, Elena Severinova, Seth A. Darst

Research output: Contribution to journalArticle

257 Scopus citations

Abstract

The 2.6 Å crystal structure of a fragment of the σ70 promoter specificity subunit of E. coli RNA polymerase is described. Residues involved in core RNA polymerase binding lie on one face of the structure. On the opposite face, aligned along one helix, are exposed residues that interact with the -10 consensus promoter element (the Pribnow box), including four aromatic residues involved in promoter melting. The structure suggests one way in which DNA interactions may be inhibited in the absence of RNA polymerase and provides a framework for the interpretation of a large number of genetic and biochemical analyses.

Original languageEnglish (US)
Pages (from-to)127-136
Number of pages10
JournalCell
Volume87
Issue number1
DOIs
StatePublished - Oct 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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