@article{5f547d61a70f43d4a6048429ad49acc9,
title = "Crystal structure of a σ70 subunit fragment from E. coli RNA polymerase",
abstract = "The 2.6 {\AA} crystal structure of a fragment of the σ70 promoter specificity subunit of E. coli RNA polymerase is described. Residues involved in core RNA polymerase binding lie on one face of the structure. On the opposite face, aligned along one helix, are exposed residues that interact with the -10 consensus promoter element (the Pribnow box), including four aromatic residues involved in promoter melting. The structure suggests one way in which DNA interactions may be inhibited in the absence of RNA polymerase and provides a framework for the interpretation of a large number of genetic and biochemical analyses.",
author = "Arun Malhotra and Elena Severinova and Darst, {Seth A.}",
note = "Funding Information: We thank J. Kuriyan and S. K. Burley and the members of their laboratories for help and encouragement, and in particular J. Goldberg for invaluable advice. We thank W. A. Hendrickson and C. Ogata for access to and support at beamline X4A, which is funded by the Howard Hughes Medical Institute at the Brookhaven National Laboratory. We also thank W. Minor for help with DENZO and M. Lonetto for communicating unpublished sequence alignment information. S. A. D. is a Lucille P. Markey Scholar and a Pew Scholar in the Biomedical Sciences. This work was supported in part by grants to S. A. D. from the Lucille P. Markey Charitable Trust, the Irma T. Hirschl Trust, the Human Frontier Science Project, the Pew Foundation, and the National Institutes of Health. ",
year = "1996",
month = oct,
doi = "10.1016/S0092-8674(00)81329-X",
language = "English (US)",
volume = "87",
pages = "127--136",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1",
}