Cry1Ac protoxin from Bacillus thuringiensis sp. kurstaki HD73 binds to surface proteins in the mouse small intestine

Roberto I. Vázquez-Padrón, Joel Gonzáles-Cabrera, Carlos García-Tovar, Leticia Neri-Bazan, Rubén Lopéz-Revilla, Manuel Hernández, Leticia Moreno-Fierro, Gustavo A. De La Riva

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49 Scopus citations

Abstract

Bacillus thuringiensis (Bt), considered a safe insecticide, produces insecticidal proteins named Cry during sporulation, which possess exceptional immunological properties. In this work using an immunohistochemical test we demonstrated that Cry1Ac protoxin (pCry1Ac) binds to the mucosal surface of the mouse small intestine. Ligand blot assay allowed us to detect, under denaturing conditions, six pCry1Ac-binding polypeptides present in brush border membrane vesicles isolated from the small intestine. Moreover, this protein induced in situ temporal changes in the electrophysiological propel ties of the mouse jejunum. The data obtained indicate a possible interaction in vivo of Cry proteins with the animal bowel which could induce changes in the physiological status of the intestine. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)54-58
Number of pages5
JournalBiochemical and biophysical research communications
Volume271
Issue number1
DOIs
StatePublished - Apr 29 2000
Externally publishedYes

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Vázquez-Padrón, R. I., Gonzáles-Cabrera, J., García-Tovar, C., Neri-Bazan, L., Lopéz-Revilla, R., Hernández, M., Moreno-Fierro, L., & De La Riva, G. A. (2000). Cry1Ac protoxin from Bacillus thuringiensis sp. kurstaki HD73 binds to surface proteins in the mouse small intestine. Biochemical and biophysical research communications, 271(1), 54-58. https://doi.org/10.1006/bbrc.2000.2584