Abstract
Background: Drug screening is rapid, inexpensive, and is often used in clinical, forensic, and workplace drug testing to gain informative results. This article seeks to determine if bupropion and/or its metabolites is resulting in false-positive amphetamine screening results in our case samples using commercially available enzymelinked immunosorbent assay tests. Method: Fortified urine and forensic case samples were used to determine crossreactivity of bupropion and its main metabolite to four different amphetamine and methamphetamine enzyme-linked immunosorbent assay kits. Results: Two of the enzyme-linked immunosorbent assay kits used to screen for amphetamine may result in false-positive results if bupropion metabolites are present in concentrations greater than 500 ng/mL. Three case samples gave a positive screen results for amphetamine using Amphetamine ULTRA kits, yet no amphetamines were confirmed by gas chromatography-mass spectrometry and all samples were positive for bupropion and metabolites. Conclusions: Laboratory directors and clinicians should be aware of the characteristic of their chosen laboratory assay and should communicate this to physicians so that results can be interpreted accurately.
| Original language | English |
|---|---|
| Pages (from-to) | 366-368 |
| Number of pages | 3 |
| Journal | Therapeutic Drug Monitoring |
| Volume | 33 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 1 2011 |
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Keywords
- Amphetamine
- Bupropion metabolite
- ELISA
- False-positive
ASJC Scopus subject areas
- Pharmacology (medical)
- Pharmacology
Cite this
Crossreactivity of bupropion metabolite with enzyme-linked immunosorbent assays designed to detect amphetamine in urine. / Reidy, Lisa; Walls, H. Chip; Steele, Bernard W.
In: Therapeutic Drug Monitoring, Vol. 33, No. 3, 01.06.2011, p. 366-368.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Crossreactivity of bupropion metabolite with enzyme-linked immunosorbent assays designed to detect amphetamine in urine
AU - Reidy, Lisa
AU - Walls, H. Chip
AU - Steele, Bernard W
PY - 2011/6/1
Y1 - 2011/6/1
N2 - Background: Drug screening is rapid, inexpensive, and is often used in clinical, forensic, and workplace drug testing to gain informative results. This article seeks to determine if bupropion and/or its metabolites is resulting in false-positive amphetamine screening results in our case samples using commercially available enzymelinked immunosorbent assay tests. Method: Fortified urine and forensic case samples were used to determine crossreactivity of bupropion and its main metabolite to four different amphetamine and methamphetamine enzyme-linked immunosorbent assay kits. Results: Two of the enzyme-linked immunosorbent assay kits used to screen for amphetamine may result in false-positive results if bupropion metabolites are present in concentrations greater than 500 ng/mL. Three case samples gave a positive screen results for amphetamine using Amphetamine ULTRA kits, yet no amphetamines were confirmed by gas chromatography-mass spectrometry and all samples were positive for bupropion and metabolites. Conclusions: Laboratory directors and clinicians should be aware of the characteristic of their chosen laboratory assay and should communicate this to physicians so that results can be interpreted accurately.
AB - Background: Drug screening is rapid, inexpensive, and is often used in clinical, forensic, and workplace drug testing to gain informative results. This article seeks to determine if bupropion and/or its metabolites is resulting in false-positive amphetamine screening results in our case samples using commercially available enzymelinked immunosorbent assay tests. Method: Fortified urine and forensic case samples were used to determine crossreactivity of bupropion and its main metabolite to four different amphetamine and methamphetamine enzyme-linked immunosorbent assay kits. Results: Two of the enzyme-linked immunosorbent assay kits used to screen for amphetamine may result in false-positive results if bupropion metabolites are present in concentrations greater than 500 ng/mL. Three case samples gave a positive screen results for amphetamine using Amphetamine ULTRA kits, yet no amphetamines were confirmed by gas chromatography-mass spectrometry and all samples were positive for bupropion and metabolites. Conclusions: Laboratory directors and clinicians should be aware of the characteristic of their chosen laboratory assay and should communicate this to physicians so that results can be interpreted accurately.
KW - Amphetamine
KW - Bupropion metabolite
KW - ELISA
KW - False-positive
UR - http://www.scopus.com/inward/record.url?scp=80051795658&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80051795658&partnerID=8YFLogxK
U2 - 10.1097/FTD.0b013e3182126d08
DO - 10.1097/FTD.0b013e3182126d08
M3 - Article
C2 - 21436763
AN - SCOPUS:80051795658
VL - 33
SP - 366
EP - 368
JO - Therapeutic Drug Monitoring
JF - Therapeutic Drug Monitoring
SN - 0163-4356
IS - 3
ER -