Crossing the blood-brain-barrier with transferrin conjugated carbon dots: A zebrafish model study

Shanghao Li, Zhili Peng, Julia Dallman, James Baker, Abdelhameed M. Othman, Patrica L. Blackwelder, Roger M. Leblanc

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Drug delivery to the central nervous system (CNS) in biological systems remains a major medical challenge due to the tight junctions between endothelial cells known as the blood-brain-barrier (BBB). Here we use a zebrafish model to explore the possibility of using transferrin-conjugated carbon dots (C-Dots) to ferry compounds across the BBB. C-Dots have previously been reported to inhibit protein fibrillation, and they are also used to deliver drugs for disease treatment. In terms of the potential medical application of C-Dots for the treatment of CNS diseases, one of the most formidable challenges is how to deliver them inside the CNS. To achieve this in this study, human transferrin was covalently conjugated to C-Dots. The conjugates were then injected into the vasculature of zebrafish to examine the possibility of crossing the BBB in vivo via transferrin receptor-mediated endocytosis. The experimental observations suggest that the transferrin-C-Dots can enter the CNS while C-Dots alone cannot.

Original languageEnglish (US)
Pages (from-to)251-256
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume145
DOIs
StatePublished - Sep 1 2016

Keywords

  • Blood-brain-barrier
  • Carbon dots
  • Central nervous system
  • Transferrin

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'Crossing the blood-brain-barrier with transferrin conjugated carbon dots: A zebrafish model study'. Together they form a unique fingerprint.

  • Cite this