Cross-species amplification of microsatellite loci in aphids: Assessment and application

Alex C.C. Wilson; Blandine Massonnet; Jean Christophe Simon; Nathalie Prunier-Leterme; Lotfali Dolatti; Kate S. Llewellyn; Christian C. Figueroa; Claudio C. Ramirez; Roger L. Blackman; Arnaud Estoup; Paul Sunnucks

doi:10.1046/j.1471-8286.2004.00584.x

Cross-species amplification of microsatellite loci in aphids: Assessment and application

Alex C.C. Wilson, Blandine Massonnet, Jean Christophe Simon, Nathalie Prunier-Leterme, Lotfali Dolatti, Kate S. Llewellyn, Christian C. Figueroa, Claudio C. Ramirez, Roger L. Blackman, Arnaud Estoup, Paul Sunnucks

Research output: Contribution to journal › Article

107 Scopus citations

Abstract

Despite the relative ease of isolating microsatellites, their development still requires substantial inputs of time, money and expertise. For this reason there is considerable interest in using existing microsatellites on species from which markers were not cloned. We tested cross-species amplification of 48 existing aphid loci in species of the following genera: Aphidinae: Aphidini: Aphis and Rhopalosiphum; Aphidinae: Macrosiphini: Acyrthosiphum, Brevicoryne, Diuraphis, Illinoia, Macrosiphoniella, Macrosiphum, Metopeurum, Metapolophium, Myzus, Phorodon, Sitobion and Uroleucon and Neuquenaphidinae: Neuquenaphis. Our results show cross-species application of known microsatellite loci is a highly promising source of codominant markers for population genetic and evolutionary studies in aphids.

Original language	English (US)
Pages (from-to)	104-109
Number of pages	6
Journal	Molecular Ecology Notes
Volume	4
Issue number	1
DOIs	https://doi.org/10.1046/j.1471-8286.2004.00584.x
State	Published - Mar 1 2004
Externally published	Yes

Keywords

Aphidini
Cross-priming
Macrosiphini
Microsatellite
Neuquenaphidinae
X-linked

ASJC Scopus subject areas

Ecology
Biochemistry, Genetics and Molecular Biology(all)
Biochemistry

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10.1046/j.1471-8286.2004.00584.x

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Wilson, A. C. C., Massonnet, B., Simon, J. C., Prunier-Leterme, N., Dolatti, L., Llewellyn, K. S., Figueroa, C. C., Ramirez, C. C., Blackman, R. L., Estoup, A., & Sunnucks, P. (2004). Cross-species amplification of microsatellite loci in aphids: Assessment and application. Molecular Ecology Notes, 4(1), 104-109. https://doi.org/10.1046/j.1471-8286.2004.00584.x

Cross-species amplification of microsatellite loci in aphids : Assessment and application. / Wilson, Alex C.C.; Massonnet, Blandine; Simon, Jean Christophe; Prunier-Leterme, Nathalie; Dolatti, Lotfali; Llewellyn, Kate S.; Figueroa, Christian C.; Ramirez, Claudio C.; Blackman, Roger L.; Estoup, Arnaud; Sunnucks, Paul.

In: Molecular Ecology Notes, Vol. 4, No. 1, 01.03.2004, p. 104-109.

Research output: Contribution to journal › Article

Wilson, Alex C.C. ; Massonnet, Blandine ; Simon, Jean Christophe ; Prunier-Leterme, Nathalie ; Dolatti, Lotfali ; Llewellyn, Kate S. ; Figueroa, Christian C. ; Ramirez, Claudio C. ; Blackman, Roger L. ; Estoup, Arnaud ; Sunnucks, Paul. / Cross-species amplification of microsatellite loci in aphids : Assessment and application. In: Molecular Ecology Notes. 2004 ; Vol. 4, No. 1. pp. 104-109.

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