Abstract
Despite the relative ease of isolating microsatellites, their development still requires substantial inputs of time, money and expertise. For this reason there is considerable interest in using existing microsatellites on species from which markers were not cloned. We tested cross-species amplification of 48 existing aphid loci in species of the following genera: Aphidinae: Aphidini: Aphis and Rhopalosiphum; Aphidinae: Macrosiphini: Acyrthosiphum, Brevicoryne, Diuraphis, Illinoia, Macrosiphoniella, Macrosiphum, Metopeurum, Metapolophium, Myzus, Phorodon, Sitobion and Uroleucon and Neuquenaphidinae: Neuquenaphis. Our results show cross-species application of known microsatellite loci is a highly promising source of codominant markers for population genetic and evolutionary studies in aphids.
| Original language | English |
|---|---|
| Pages (from-to) | 104-109 |
| Number of pages | 6 |
| Journal | Molecular Ecology Notes |
| Volume | 4 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 1 2004 |
| Externally published | Yes |
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Keywords
- Aphidini
- Cross-priming
- Macrosiphini
- Microsatellite
- Neuquenaphidinae
- X-linked
ASJC Scopus subject areas
- Ecology
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
Cite this
Cross-species amplification of microsatellite loci in aphids : Assessment and application. / Wilson, Alexandra; Massonnet, Blandine; Simon, Jean Christophe; Prunier-Leterme, Nathalie; Dolatti, Lotfali; Llewellyn, Kate S.; Figueroa, Christian C.; Ramirez, Claudio C.; Blackman, Roger L.; Estoup, Arnaud; Sunnucks, Paul.
In: Molecular Ecology Notes, Vol. 4, No. 1, 01.03.2004, p. 104-109.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cross-species amplification of microsatellite loci in aphids
T2 - Assessment and application
AU - Wilson, Alexandra
AU - Massonnet, Blandine
AU - Simon, Jean Christophe
AU - Prunier-Leterme, Nathalie
AU - Dolatti, Lotfali
AU - Llewellyn, Kate S.
AU - Figueroa, Christian C.
AU - Ramirez, Claudio C.
AU - Blackman, Roger L.
AU - Estoup, Arnaud
AU - Sunnucks, Paul
PY - 2004/3/1
Y1 - 2004/3/1
N2 - Despite the relative ease of isolating microsatellites, their development still requires substantial inputs of time, money and expertise. For this reason there is considerable interest in using existing microsatellites on species from which markers were not cloned. We tested cross-species amplification of 48 existing aphid loci in species of the following genera: Aphidinae: Aphidini: Aphis and Rhopalosiphum; Aphidinae: Macrosiphini: Acyrthosiphum, Brevicoryne, Diuraphis, Illinoia, Macrosiphoniella, Macrosiphum, Metopeurum, Metapolophium, Myzus, Phorodon, Sitobion and Uroleucon and Neuquenaphidinae: Neuquenaphis. Our results show cross-species application of known microsatellite loci is a highly promising source of codominant markers for population genetic and evolutionary studies in aphids.
AB - Despite the relative ease of isolating microsatellites, their development still requires substantial inputs of time, money and expertise. For this reason there is considerable interest in using existing microsatellites on species from which markers were not cloned. We tested cross-species amplification of 48 existing aphid loci in species of the following genera: Aphidinae: Aphidini: Aphis and Rhopalosiphum; Aphidinae: Macrosiphini: Acyrthosiphum, Brevicoryne, Diuraphis, Illinoia, Macrosiphoniella, Macrosiphum, Metopeurum, Metapolophium, Myzus, Phorodon, Sitobion and Uroleucon and Neuquenaphidinae: Neuquenaphis. Our results show cross-species application of known microsatellite loci is a highly promising source of codominant markers for population genetic and evolutionary studies in aphids.
KW - Aphidini
KW - Cross-priming
KW - Macrosiphini
KW - Microsatellite
KW - Neuquenaphidinae
KW - X-linked
UR - http://www.scopus.com/inward/record.url?scp=1342267273&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1342267273&partnerID=8YFLogxK
U2 - 10.1046/j.1471-8286.2004.00584.x
DO - 10.1046/j.1471-8286.2004.00584.x
M3 - Article
AN - SCOPUS:1342267273
VL - 4
SP - 104
EP - 109
JO - Molecular Ecology Resources
JF - Molecular Ecology Resources
SN - 1755-098X
IS - 1
ER -