Cross-species amplification of microsatellite loci in aphids: Assessment and application

Alex C.C. Wilson, Blandine Massonnet, Jean Christophe Simon, Nathalie Prunier-Leterme, Lotfali Dolatti, Kate S. Llewellyn, Christian C. Figueroa, Claudio C. Ramirez, Roger L. Blackman, Arnaud Estoup, Paul Sunnucks

Research output: Contribution to journalArticle

107 Scopus citations

Abstract

Despite the relative ease of isolating microsatellites, their development still requires substantial inputs of time, money and expertise. For this reason there is considerable interest in using existing microsatellites on species from which markers were not cloned. We tested cross-species amplification of 48 existing aphid loci in species of the following genera: Aphidinae: Aphidini: Aphis and Rhopalosiphum; Aphidinae: Macrosiphini: Acyrthosiphum, Brevicoryne, Diuraphis, Illinoia, Macrosiphoniella, Macrosiphum, Metopeurum, Metapolophium, Myzus, Phorodon, Sitobion and Uroleucon and Neuquenaphidinae: Neuquenaphis. Our results show cross-species application of known microsatellite loci is a highly promising source of codominant markers for population genetic and evolutionary studies in aphids.

Original languageEnglish (US)
Pages (from-to)104-109
Number of pages6
JournalMolecular Ecology Notes
Volume4
Issue number1
DOIs
StatePublished - Mar 1 2004
Externally publishedYes

Keywords

  • Aphidini
  • Cross-priming
  • Macrosiphini
  • Microsatellite
  • Neuquenaphidinae
  • X-linked

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

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  • Cite this

    Wilson, A. C. C., Massonnet, B., Simon, J. C., Prunier-Leterme, N., Dolatti, L., Llewellyn, K. S., Figueroa, C. C., Ramirez, C. C., Blackman, R. L., Estoup, A., & Sunnucks, P. (2004). Cross-species amplification of microsatellite loci in aphids: Assessment and application. Molecular Ecology Notes, 4(1), 104-109. https://doi.org/10.1046/j.1471-8286.2004.00584.x