Cross-species amplification of microsatellite loci in aphids: Assessment and application

Alexandra Wilson, Blandine Massonnet, Jean Christophe Simon, Nathalie Prunier-Leterme, Lotfali Dolatti, Kate S. Llewellyn, Christian C. Figueroa, Claudio C. Ramirez, Roger L. Blackman, Arnaud Estoup, Paul Sunnucks

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Despite the relative ease of isolating microsatellites, their development still requires substantial inputs of time, money and expertise. For this reason there is considerable interest in using existing microsatellites on species from which markers were not cloned. We tested cross-species amplification of 48 existing aphid loci in species of the following genera: Aphidinae: Aphidini: Aphis and Rhopalosiphum; Aphidinae: Macrosiphini: Acyrthosiphum, Brevicoryne, Diuraphis, Illinoia, Macrosiphoniella, Macrosiphum, Metopeurum, Metapolophium, Myzus, Phorodon, Sitobion and Uroleucon and Neuquenaphidinae: Neuquenaphis. Our results show cross-species application of known microsatellite loci is a highly promising source of codominant markers for population genetic and evolutionary studies in aphids.

Original languageEnglish
Pages (from-to)104-109
Number of pages6
JournalMolecular Ecology Notes
Volume4
Issue number1
DOIs
StatePublished - Mar 1 2004
Externally publishedYes

Fingerprint

Aphids
aphid
Microsatellite Repeats
Amplification
Aphidoidea
amplification
microsatellite repeats
Neuquenaphis
Phorodon
Brevicoryne
Illinoia
loci
Rhopalosiphum
Diuraphis
Macrosiphoniella
Sitobion
Macrosiphum
Myzus
Uroleucon
Aphis (Aphididae)

Keywords

  • Aphidini
  • Cross-priming
  • Macrosiphini
  • Microsatellite
  • Neuquenaphidinae
  • X-linked

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Wilson, A., Massonnet, B., Simon, J. C., Prunier-Leterme, N., Dolatti, L., Llewellyn, K. S., ... Sunnucks, P. (2004). Cross-species amplification of microsatellite loci in aphids: Assessment and application. Molecular Ecology Notes, 4(1), 104-109. https://doi.org/10.1046/j.1471-8286.2004.00584.x

Cross-species amplification of microsatellite loci in aphids : Assessment and application. / Wilson, Alexandra; Massonnet, Blandine; Simon, Jean Christophe; Prunier-Leterme, Nathalie; Dolatti, Lotfali; Llewellyn, Kate S.; Figueroa, Christian C.; Ramirez, Claudio C.; Blackman, Roger L.; Estoup, Arnaud; Sunnucks, Paul.

In: Molecular Ecology Notes, Vol. 4, No. 1, 01.03.2004, p. 104-109.

Research output: Contribution to journalArticle

Wilson, A, Massonnet, B, Simon, JC, Prunier-Leterme, N, Dolatti, L, Llewellyn, KS, Figueroa, CC, Ramirez, CC, Blackman, RL, Estoup, A & Sunnucks, P 2004, 'Cross-species amplification of microsatellite loci in aphids: Assessment and application', Molecular Ecology Notes, vol. 4, no. 1, pp. 104-109. https://doi.org/10.1046/j.1471-8286.2004.00584.x
Wilson, Alexandra ; Massonnet, Blandine ; Simon, Jean Christophe ; Prunier-Leterme, Nathalie ; Dolatti, Lotfali ; Llewellyn, Kate S. ; Figueroa, Christian C. ; Ramirez, Claudio C. ; Blackman, Roger L. ; Estoup, Arnaud ; Sunnucks, Paul. / Cross-species amplification of microsatellite loci in aphids : Assessment and application. In: Molecular Ecology Notes. 2004 ; Vol. 4, No. 1. pp. 104-109.
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