Cross-sensitization of histamine-independent itch in mouse primary sensory neurons

Tasuku Akiyama, M. Tominaga, A. Davoodi, M. Nagamine, K. Blansit, A. Horwitz, M. I. Carstens, E. Carstens

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Overexpression of pruritogens and their precursors may contribute to the sensitization of histamine-dependent and -independent itch-signaling pathways in chronic itch. We presently investigated self- and cross-sensitization of scratching behavior elicited by various pruritogens, and their effects on primary sensory neurons. The MrgprC11 agonist BAM8-22 exhibited self- and reciprocal cross-sensitization of scratching evoked by the protease-activated receptor-2 (PAR-2) agonist SLIGRL. The MrgprA3 agonist chloroquine unidirectionally cross-sensitized BAM8-22-evoked scratching. Histamine unidirectionally cross-sensitized scratching evoked by chloroquine and BAM8-22. SLIGRL unidirectionally cross-sensitized scratching evoked by chloroquine. Dorsal root ganglion (DRG) cells responded to various combinations of pruritogens and algogens. Neither chloroquine, BAM8-22 nor histamine had any effect on responses of DRG cell responses to subsequently applied pruritogens, implying that their behavioral self- and cross-sensitization effects are mediated indirectly. SLIGRL unilaterally cross-sensitized responses of DRG cells to chloroquine and BAM8-22, consistent with the behavioral data. These results indicate that unidirectional cross-sensitization of histamine-independent itch-signaling pathways might occur at a peripheral site through PAR-2. PAR-2 expressed in pruriceptive nerve endings is a potential target to reduce sensitization associated with chronic itch.

Original languageEnglish (US)
Pages (from-to)305-312
Number of pages8
JournalNeuroscience
Volume226
DOIs
StatePublished - Dec 13 2012
Externally publishedYes

Fingerprint

seryl-leucyl-isoleucyl-glycyl-arginyl-leucine
Chloroquine
Sensory Receptor Cells
Histamine
PAR-2 Receptor
Spinal Ganglia
Nerve Endings
bovine adrenal medulla 8-22

Keywords

  • Calcium imaging
  • Cross-sensitization
  • Dorsal root ganglion (DRG) cell
  • Itch
  • Pruritogen
  • Scratching

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Akiyama, T., Tominaga, M., Davoodi, A., Nagamine, M., Blansit, K., Horwitz, A., ... Carstens, E. (2012). Cross-sensitization of histamine-independent itch in mouse primary sensory neurons. Neuroscience, 226, 305-312. https://doi.org/10.1016/j.neuroscience.2012.09.019

Cross-sensitization of histamine-independent itch in mouse primary sensory neurons. / Akiyama, Tasuku; Tominaga, M.; Davoodi, A.; Nagamine, M.; Blansit, K.; Horwitz, A.; Carstens, M. I.; Carstens, E.

In: Neuroscience, Vol. 226, 13.12.2012, p. 305-312.

Research output: Contribution to journalArticle

Akiyama, T, Tominaga, M, Davoodi, A, Nagamine, M, Blansit, K, Horwitz, A, Carstens, MI & Carstens, E 2012, 'Cross-sensitization of histamine-independent itch in mouse primary sensory neurons', Neuroscience, vol. 226, pp. 305-312. https://doi.org/10.1016/j.neuroscience.2012.09.019
Akiyama, Tasuku ; Tominaga, M. ; Davoodi, A. ; Nagamine, M. ; Blansit, K. ; Horwitz, A. ; Carstens, M. I. ; Carstens, E. / Cross-sensitization of histamine-independent itch in mouse primary sensory neurons. In: Neuroscience. 2012 ; Vol. 226. pp. 305-312.
@article{2f935083389349b3b51abc1e3c08e8d4,
title = "Cross-sensitization of histamine-independent itch in mouse primary sensory neurons",
abstract = "Overexpression of pruritogens and their precursors may contribute to the sensitization of histamine-dependent and -independent itch-signaling pathways in chronic itch. We presently investigated self- and cross-sensitization of scratching behavior elicited by various pruritogens, and their effects on primary sensory neurons. The MrgprC11 agonist BAM8-22 exhibited self- and reciprocal cross-sensitization of scratching evoked by the protease-activated receptor-2 (PAR-2) agonist SLIGRL. The MrgprA3 agonist chloroquine unidirectionally cross-sensitized BAM8-22-evoked scratching. Histamine unidirectionally cross-sensitized scratching evoked by chloroquine and BAM8-22. SLIGRL unidirectionally cross-sensitized scratching evoked by chloroquine. Dorsal root ganglion (DRG) cells responded to various combinations of pruritogens and algogens. Neither chloroquine, BAM8-22 nor histamine had any effect on responses of DRG cell responses to subsequently applied pruritogens, implying that their behavioral self- and cross-sensitization effects are mediated indirectly. SLIGRL unilaterally cross-sensitized responses of DRG cells to chloroquine and BAM8-22, consistent with the behavioral data. These results indicate that unidirectional cross-sensitization of histamine-independent itch-signaling pathways might occur at a peripheral site through PAR-2. PAR-2 expressed in pruriceptive nerve endings is a potential target to reduce sensitization associated with chronic itch.",
keywords = "Calcium imaging, Cross-sensitization, Dorsal root ganglion (DRG) cell, Itch, Pruritogen, Scratching",
author = "Tasuku Akiyama and M. Tominaga and A. Davoodi and M. Nagamine and K. Blansit and A. Horwitz and Carstens, {M. I.} and E. Carstens",
year = "2012",
month = "12",
day = "13",
doi = "10.1016/j.neuroscience.2012.09.019",
language = "English (US)",
volume = "226",
pages = "305--312",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Cross-sensitization of histamine-independent itch in mouse primary sensory neurons

AU - Akiyama, Tasuku

AU - Tominaga, M.

AU - Davoodi, A.

AU - Nagamine, M.

AU - Blansit, K.

AU - Horwitz, A.

AU - Carstens, M. I.

AU - Carstens, E.

PY - 2012/12/13

Y1 - 2012/12/13

N2 - Overexpression of pruritogens and their precursors may contribute to the sensitization of histamine-dependent and -independent itch-signaling pathways in chronic itch. We presently investigated self- and cross-sensitization of scratching behavior elicited by various pruritogens, and their effects on primary sensory neurons. The MrgprC11 agonist BAM8-22 exhibited self- and reciprocal cross-sensitization of scratching evoked by the protease-activated receptor-2 (PAR-2) agonist SLIGRL. The MrgprA3 agonist chloroquine unidirectionally cross-sensitized BAM8-22-evoked scratching. Histamine unidirectionally cross-sensitized scratching evoked by chloroquine and BAM8-22. SLIGRL unidirectionally cross-sensitized scratching evoked by chloroquine. Dorsal root ganglion (DRG) cells responded to various combinations of pruritogens and algogens. Neither chloroquine, BAM8-22 nor histamine had any effect on responses of DRG cell responses to subsequently applied pruritogens, implying that their behavioral self- and cross-sensitization effects are mediated indirectly. SLIGRL unilaterally cross-sensitized responses of DRG cells to chloroquine and BAM8-22, consistent with the behavioral data. These results indicate that unidirectional cross-sensitization of histamine-independent itch-signaling pathways might occur at a peripheral site through PAR-2. PAR-2 expressed in pruriceptive nerve endings is a potential target to reduce sensitization associated with chronic itch.

AB - Overexpression of pruritogens and their precursors may contribute to the sensitization of histamine-dependent and -independent itch-signaling pathways in chronic itch. We presently investigated self- and cross-sensitization of scratching behavior elicited by various pruritogens, and their effects on primary sensory neurons. The MrgprC11 agonist BAM8-22 exhibited self- and reciprocal cross-sensitization of scratching evoked by the protease-activated receptor-2 (PAR-2) agonist SLIGRL. The MrgprA3 agonist chloroquine unidirectionally cross-sensitized BAM8-22-evoked scratching. Histamine unidirectionally cross-sensitized scratching evoked by chloroquine and BAM8-22. SLIGRL unidirectionally cross-sensitized scratching evoked by chloroquine. Dorsal root ganglion (DRG) cells responded to various combinations of pruritogens and algogens. Neither chloroquine, BAM8-22 nor histamine had any effect on responses of DRG cell responses to subsequently applied pruritogens, implying that their behavioral self- and cross-sensitization effects are mediated indirectly. SLIGRL unilaterally cross-sensitized responses of DRG cells to chloroquine and BAM8-22, consistent with the behavioral data. These results indicate that unidirectional cross-sensitization of histamine-independent itch-signaling pathways might occur at a peripheral site through PAR-2. PAR-2 expressed in pruriceptive nerve endings is a potential target to reduce sensitization associated with chronic itch.

KW - Calcium imaging

KW - Cross-sensitization

KW - Dorsal root ganglion (DRG) cell

KW - Itch

KW - Pruritogen

KW - Scratching

UR - http://www.scopus.com/inward/record.url?scp=84867309901&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867309901&partnerID=8YFLogxK

U2 - 10.1016/j.neuroscience.2012.09.019

DO - 10.1016/j.neuroscience.2012.09.019

M3 - Article

C2 - 23000623

AN - SCOPUS:84867309901

VL - 226

SP - 305

EP - 312

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

ER -