Cross-reactivity of anti-chicken IgY antibody with immunoglobulins of exotic avian species

Carolyn Cray, David Villar

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Background: A major challenge in the serologic diagnosis of infectious diseases in exotic birds is the limited availability of species-specific antibodies. Objectives: The purpose of the current study was to determine if there is cross reactivity between commercially available anti-chicken IgY antibodies and immunoglobulins of several avian species, with particular emphasis on psittacines. Methods: To quantitate the reactivity with anti-chicken IgY, Western blot analysis was performed using plasma samples from many different avian species. Results were compared with gamma globulin fraction quantitation obtained by protein electrophoresis. Results: By Western blot, 2 protein bands corresponding to the heavy and light chains of chicken IgY were identified in species from 21 avian orders using 1 of 2 rabbit anti-chicken IgY antibodies. Densitometric analysis showed that the amount of immunoglobulin estimated from Western blots correlated strongly with data from protein electrophoresis assays. Conclusions: The results demonstrate that some commercially available anti-chicken IgY antibodies exhibit good cross-reactivity with most avian species.

Original languageEnglish (US)
Pages (from-to)328-331
Number of pages4
JournalVeterinary Clinical Pathology
Volume37
Issue number3
DOIs
StatePublished - Sep 1 2008

Keywords

  • Avian
  • Chicken
  • IgY
  • Immunology
  • Protein electrophoresis
  • Western blot

ASJC Scopus subject areas

  • veterinary(all)

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