Cross-reactive recognition of alloantigens and certain B6 mutant antigens by antigen-specific and polyclonally activated cytotoxic effector cells

Ursula Hurtenbach, Gene M. Shearer, Robert B Levy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Various B6 cytotoxic T lymphocyte (CTL) effector populations were tested for cross-reactive lysis (CRL) of unmodified third-party allogeneic as well as of various Kbm and Dbm mutant target cells. The effector cells were generated by in vitro stimulation of B6 spleen cells either from unprimed mice against alloantigen, Con A, or a pool of different allogeneic stimulators, or from in vivo hapten-self primed mice against trinitrophenyl (TNP)-self or N-(5-sulfonic-1-naphthyl)ethylenediame (AED)-self antigens. Except for AED-primed effector cells all CTL populations exhibited significant CRL on third-party allogeneic target cells. More importantly, these alloreactive and H-2-restricted effectors also lysed some of the Kb mutants, but not others. Thus, unmodified bm1 and bm11 mutants were lysed by all of the B6 wild-type effectors, whereas bm6 and bm9 were only weakly lysed by alloreactive CTL. None of the B6 CTL populations lysed bm13 and bm14 target cells. These data indicate that (a) alloreactive and self-restricted CTL can recognize the same molecular structure of Class I antigens although the epitopes may be different; (b) CTL discriminate between a few amino acid substitutions in certain positions of the H-2 molecule, and (c) the newly generated antigenic determinants of the bm mutants allow them to be divided into three categories in terms of their allogenicity for these cross-reactive CTL populations.

Original languageEnglish
Pages (from-to)163-171
Number of pages9
JournalCellular Immunology
Volume80
Issue number1
DOIs
StatePublished - Jan 1 1983
Externally publishedYes

Fingerprint

Isoantigens
Cytotoxic T-Lymphocytes
Antigens
Population
Epitopes
Histocompatibility Antigens Class I
Haptens
Autoantigens
Amino Acid Substitution
Molecular Structure
Spleen

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Cross-reactive recognition of alloantigens and certain B6 mutant antigens by antigen-specific and polyclonally activated cytotoxic effector cells. / Hurtenbach, Ursula; Shearer, Gene M.; Levy, Robert B.

In: Cellular Immunology, Vol. 80, No. 1, 01.01.1983, p. 163-171.

Research output: Contribution to journalArticle

@article{373fcc9c59b14abca4f602f2ca5d074c,
title = "Cross-reactive recognition of alloantigens and certain B6 mutant antigens by antigen-specific and polyclonally activated cytotoxic effector cells",
abstract = "Various B6 cytotoxic T lymphocyte (CTL) effector populations were tested for cross-reactive lysis (CRL) of unmodified third-party allogeneic as well as of various Kbm and Dbm mutant target cells. The effector cells were generated by in vitro stimulation of B6 spleen cells either from unprimed mice against alloantigen, Con A, or a pool of different allogeneic stimulators, or from in vivo hapten-self primed mice against trinitrophenyl (TNP)-self or N-(5-sulfonic-1-naphthyl)ethylenediame (AED)-self antigens. Except for AED-primed effector cells all CTL populations exhibited significant CRL on third-party allogeneic target cells. More importantly, these alloreactive and H-2-restricted effectors also lysed some of the Kb mutants, but not others. Thus, unmodified bm1 and bm11 mutants were lysed by all of the B6 wild-type effectors, whereas bm6 and bm9 were only weakly lysed by alloreactive CTL. None of the B6 CTL populations lysed bm13 and bm14 target cells. These data indicate that (a) alloreactive and self-restricted CTL can recognize the same molecular structure of Class I antigens although the epitopes may be different; (b) CTL discriminate between a few amino acid substitutions in certain positions of the H-2 molecule, and (c) the newly generated antigenic determinants of the bm mutants allow them to be divided into three categories in terms of their allogenicity for these cross-reactive CTL populations.",
author = "Ursula Hurtenbach and Shearer, {Gene M.} and Levy, {Robert B}",
year = "1983",
month = "1",
day = "1",
doi = "10.1016/0008-8749(83)90103-X",
language = "English",
volume = "80",
pages = "163--171",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Cross-reactive recognition of alloantigens and certain B6 mutant antigens by antigen-specific and polyclonally activated cytotoxic effector cells

AU - Hurtenbach, Ursula

AU - Shearer, Gene M.

AU - Levy, Robert B

PY - 1983/1/1

Y1 - 1983/1/1

N2 - Various B6 cytotoxic T lymphocyte (CTL) effector populations were tested for cross-reactive lysis (CRL) of unmodified third-party allogeneic as well as of various Kbm and Dbm mutant target cells. The effector cells were generated by in vitro stimulation of B6 spleen cells either from unprimed mice against alloantigen, Con A, or a pool of different allogeneic stimulators, or from in vivo hapten-self primed mice against trinitrophenyl (TNP)-self or N-(5-sulfonic-1-naphthyl)ethylenediame (AED)-self antigens. Except for AED-primed effector cells all CTL populations exhibited significant CRL on third-party allogeneic target cells. More importantly, these alloreactive and H-2-restricted effectors also lysed some of the Kb mutants, but not others. Thus, unmodified bm1 and bm11 mutants were lysed by all of the B6 wild-type effectors, whereas bm6 and bm9 were only weakly lysed by alloreactive CTL. None of the B6 CTL populations lysed bm13 and bm14 target cells. These data indicate that (a) alloreactive and self-restricted CTL can recognize the same molecular structure of Class I antigens although the epitopes may be different; (b) CTL discriminate between a few amino acid substitutions in certain positions of the H-2 molecule, and (c) the newly generated antigenic determinants of the bm mutants allow them to be divided into three categories in terms of their allogenicity for these cross-reactive CTL populations.

AB - Various B6 cytotoxic T lymphocyte (CTL) effector populations were tested for cross-reactive lysis (CRL) of unmodified third-party allogeneic as well as of various Kbm and Dbm mutant target cells. The effector cells were generated by in vitro stimulation of B6 spleen cells either from unprimed mice against alloantigen, Con A, or a pool of different allogeneic stimulators, or from in vivo hapten-self primed mice against trinitrophenyl (TNP)-self or N-(5-sulfonic-1-naphthyl)ethylenediame (AED)-self antigens. Except for AED-primed effector cells all CTL populations exhibited significant CRL on third-party allogeneic target cells. More importantly, these alloreactive and H-2-restricted effectors also lysed some of the Kb mutants, but not others. Thus, unmodified bm1 and bm11 mutants were lysed by all of the B6 wild-type effectors, whereas bm6 and bm9 were only weakly lysed by alloreactive CTL. None of the B6 CTL populations lysed bm13 and bm14 target cells. These data indicate that (a) alloreactive and self-restricted CTL can recognize the same molecular structure of Class I antigens although the epitopes may be different; (b) CTL discriminate between a few amino acid substitutions in certain positions of the H-2 molecule, and (c) the newly generated antigenic determinants of the bm mutants allow them to be divided into three categories in terms of their allogenicity for these cross-reactive CTL populations.

UR - http://www.scopus.com/inward/record.url?scp=0020551249&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020551249&partnerID=8YFLogxK

U2 - 10.1016/0008-8749(83)90103-X

DO - 10.1016/0008-8749(83)90103-X

M3 - Article

C2 - 6191874

AN - SCOPUS:0020551249

VL - 80

SP - 163

EP - 171

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -