Cross-linking of cell surface receptors enhances cooperativity of molecular adhesion

Aileen Chen, Vincent T. Moy

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Cooperativity of molecular adhesion has been proposed as a mechanism for enhanced binding strength of adhesion molecules on the cell surface. Direct evidence for its mechanism, however, has been lacking until now. Atomic force microscopy (AFM) was used to measure the adhesive strength between concanavalin A (Con A) coupled to an AFM tip and Con A receptors on the surface of NIH3T3 fibroblast cells. Cross-linking of receptors with either glutaraldehyde or 3,3'-dithio-bis(sulfosuccinimidylproprionate) (DTSSP) led to an increase in adhesion that could be attributed to enhanced cooperativity among adhesion complexes. An increase in loading rate due to greater stiffness of fixed cells also contributed to the twofold increase in binding strength. These results show that receptor cross-linking can greatly contribute to a total increase in cell adhesion by creating a shift toward cooperative binding of receptors.

Original languageEnglish
Pages (from-to)2814-2820
Number of pages7
JournalBiophysical Journal
Volume78
Issue number6
StatePublished - Jun 1 2000

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Atomic Force Microscopy
Cell Surface Receptors
Concanavalin A Receptors
Cell Adhesion Molecules
Glutaral
Concanavalin A
Cell Adhesion
Adhesives
Fibroblasts

ASJC Scopus subject areas

  • Biophysics

Cite this

Cross-linking of cell surface receptors enhances cooperativity of molecular adhesion. / Chen, Aileen; Moy, Vincent T.

In: Biophysical Journal, Vol. 78, No. 6, 01.06.2000, p. 2814-2820.

Research output: Contribution to journalArticle

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