CRISPR: a versatile tool for both forward and reverse genetics research

Channabasavaiah B. Gurumurthy, M’hamed Grati, Masato Ohtsuka, Samantha L P Schilit, Rolen M. Quadros, Xue Z Liu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Human genetics research employs the two opposing approaches of forward and reverse genetics. While forward genetics identifies and links a mutation to an observed disease etiology, reverse genetics induces mutations in model organisms to study their role in disease. In most cases, causality for mutations identified by forward genetics is confirmed by reverse genetics through the development of genetically engineered animal models and an assessment of whether the model can recapitulate the disease. While many technological advances have helped improve these approaches, some gaps still remain. CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated), which has emerged as a revolutionary genetic engineering tool, holds great promise for closing such gaps. By combining the benefits of forward and reverse genetics, it has dramatically expedited human genetics research. We provide a perspective on the power of CRISPR-based forward and reverse genetics tools in human genetics and discuss its applications using some disease examples.

Original languageEnglish (US)
Pages (from-to)971-976
Number of pages6
JournalHuman Genetics
Volume135
Issue number9
DOIs
StatePublished - Sep 1 2016

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Clustered Regularly Interspaced Short Palindromic Repeats
Reverse Genetics
Genetic Research
Medical Genetics
Mutation
Genetically Modified Animals
Genetic Engineering
Causality
Animal Models

ASJC Scopus subject areas

  • Medicine(all)
  • Genetics
  • Genetics(clinical)

Cite this

Gurumurthy, C. B., Grati, M., Ohtsuka, M., Schilit, S. L. P., Quadros, R. M., & Liu, X. Z. (2016). CRISPR: a versatile tool for both forward and reverse genetics research. Human Genetics, 135(9), 971-976. https://doi.org/10.1007/s00439-016-1704-4

CRISPR : a versatile tool for both forward and reverse genetics research. / Gurumurthy, Channabasavaiah B.; Grati, M’hamed; Ohtsuka, Masato; Schilit, Samantha L P; Quadros, Rolen M.; Liu, Xue Z.

In: Human Genetics, Vol. 135, No. 9, 01.09.2016, p. 971-976.

Research output: Contribution to journalArticle

Gurumurthy, CB, Grati, M, Ohtsuka, M, Schilit, SLP, Quadros, RM & Liu, XZ 2016, 'CRISPR: a versatile tool for both forward and reverse genetics research', Human Genetics, vol. 135, no. 9, pp. 971-976. https://doi.org/10.1007/s00439-016-1704-4
Gurumurthy CB, Grati M, Ohtsuka M, Schilit SLP, Quadros RM, Liu XZ. CRISPR: a versatile tool for both forward and reverse genetics research. Human Genetics. 2016 Sep 1;135(9):971-976. https://doi.org/10.1007/s00439-016-1704-4
Gurumurthy, Channabasavaiah B. ; Grati, M’hamed ; Ohtsuka, Masato ; Schilit, Samantha L P ; Quadros, Rolen M. ; Liu, Xue Z. / CRISPR : a versatile tool for both forward and reverse genetics research. In: Human Genetics. 2016 ; Vol. 135, No. 9. pp. 971-976.
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