Creation of chimeric mutant axolotls: A model to study early embryonic heart development in Mexican axolotls

L. F. Lemanski, F. Meng, S. L. Lemanski, N. Dawson, Chi Zhang, D. Foster, Q. Li, M. Nakatsugawa, R. W. Zajdel, D. K. Dube, X. Huang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The Mexican axolotl (Ambystoma mexicanum) provides an excellent model for studying heart development since it carries a cardiac lethal mutation in gene c that results in failure of contraction of mutant embryonic myocardium. In cardiac mutant axolotls (c/c) the hearts do not beat, apparently because of an absence of organized myofibrils. To date, there has been no way to analyze the genotypes of embryos from heterozygous spawnings (+/c×+/c) until stage 35 when the normal (+/c or +/+) embryos first begin to have beating hearts; mutant (c/c) embryos fail to develop normal heartbeats. In the present study, we created chimeric axolotls by using microsurgical techniques. The general approach was to transect tailbud embryos and join the anterior and posterior halves of two different individuals. The chimeric axolotl is composed of a normal head and heart region (+/+), permitting survival and a mutant body containing mutant gonads (c/c) that permits the production of c/c mutant offspring: 100% c/c offspring were obtained by mating c/c chimeras (c/c×c/c). The mutant phenotypes were confirmed by the absence of beating hearts and death at stage 41 in 100% of the embryos. Examination of the mutant hearts with electron microscopy and confocal microscopy after immunofluorescent staining for tropomyosin showed identical images to those described previously in naturally-occurring c/c mutant axolotls (i.e., lacking organized sarcomeric myofibrils). These "c/c chimeric" axolotls provide a useful and unique way to investigate early embryonic heart development in cardiac mutant Mexican axolotls.

Original languageEnglish
Pages (from-to)335-342
Number of pages8
JournalAnatomy and Embryology
Volume203
Issue number5
DOIs
StatePublished - Jun 6 2001
Externally publishedYes

Fingerprint

Ambystoma mexicanum
Embryonic Development
Embryonic Structures
Myofibrils
Tropomyosin
Gonads
Confocal Microscopy
Myocardium
Electron Microscopy
Head
Genotype
Staining and Labeling
Phenotype
Mutation

Keywords

  • Animal
  • Chimera
  • Gene
  • Mutation
  • Myocardium

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology
  • Embryology

Cite this

Lemanski, L. F., Meng, F., Lemanski, S. L., Dawson, N., Zhang, C., Foster, D., ... Huang, X. (2001). Creation of chimeric mutant axolotls: A model to study early embryonic heart development in Mexican axolotls. Anatomy and Embryology, 203(5), 335-342. https://doi.org/10.1007/s004290100158

Creation of chimeric mutant axolotls : A model to study early embryonic heart development in Mexican axolotls. / Lemanski, L. F.; Meng, F.; Lemanski, S. L.; Dawson, N.; Zhang, Chi; Foster, D.; Li, Q.; Nakatsugawa, M.; Zajdel, R. W.; Dube, D. K.; Huang, X.

In: Anatomy and Embryology, Vol. 203, No. 5, 06.06.2001, p. 335-342.

Research output: Contribution to journalArticle

Lemanski, LF, Meng, F, Lemanski, SL, Dawson, N, Zhang, C, Foster, D, Li, Q, Nakatsugawa, M, Zajdel, RW, Dube, DK & Huang, X 2001, 'Creation of chimeric mutant axolotls: A model to study early embryonic heart development in Mexican axolotls', Anatomy and Embryology, vol. 203, no. 5, pp. 335-342. https://doi.org/10.1007/s004290100158
Lemanski, L. F. ; Meng, F. ; Lemanski, S. L. ; Dawson, N. ; Zhang, Chi ; Foster, D. ; Li, Q. ; Nakatsugawa, M. ; Zajdel, R. W. ; Dube, D. K. ; Huang, X. / Creation of chimeric mutant axolotls : A model to study early embryonic heart development in Mexican axolotls. In: Anatomy and Embryology. 2001 ; Vol. 203, No. 5. pp. 335-342.
@article{f8019eeac6ae4f2ea6d0ffbce12e9500,
title = "Creation of chimeric mutant axolotls: A model to study early embryonic heart development in Mexican axolotls",
abstract = "The Mexican axolotl (Ambystoma mexicanum) provides an excellent model for studying heart development since it carries a cardiac lethal mutation in gene c that results in failure of contraction of mutant embryonic myocardium. In cardiac mutant axolotls (c/c) the hearts do not beat, apparently because of an absence of organized myofibrils. To date, there has been no way to analyze the genotypes of embryos from heterozygous spawnings (+/c×+/c) until stage 35 when the normal (+/c or +/+) embryos first begin to have beating hearts; mutant (c/c) embryos fail to develop normal heartbeats. In the present study, we created chimeric axolotls by using microsurgical techniques. The general approach was to transect tailbud embryos and join the anterior and posterior halves of two different individuals. The chimeric axolotl is composed of a normal head and heart region (+/+), permitting survival and a mutant body containing mutant gonads (c/c) that permits the production of c/c mutant offspring: 100{\%} c/c offspring were obtained by mating c/c chimeras (c/c×c/c). The mutant phenotypes were confirmed by the absence of beating hearts and death at stage 41 in 100{\%} of the embryos. Examination of the mutant hearts with electron microscopy and confocal microscopy after immunofluorescent staining for tropomyosin showed identical images to those described previously in naturally-occurring c/c mutant axolotls (i.e., lacking organized sarcomeric myofibrils). These {"}c/c chimeric{"} axolotls provide a useful and unique way to investigate early embryonic heart development in cardiac mutant Mexican axolotls.",
keywords = "Animal, Chimera, Gene, Mutation, Myocardium",
author = "Lemanski, {L. F.} and F. Meng and Lemanski, {S. L.} and N. Dawson and Chi Zhang and D. Foster and Q. Li and M. Nakatsugawa and Zajdel, {R. W.} and Dube, {D. K.} and X. Huang",
year = "2001",
month = "6",
day = "6",
doi = "10.1007/s004290100158",
language = "English",
volume = "203",
pages = "335--342",
journal = "Referate und Beiträge zur Anatomie und Entwickelungsgeschichte",
issn = "0177-5154",
publisher = "Springer Verlag",
number = "5",

}

TY - JOUR

T1 - Creation of chimeric mutant axolotls

T2 - A model to study early embryonic heart development in Mexican axolotls

AU - Lemanski, L. F.

AU - Meng, F.

AU - Lemanski, S. L.

AU - Dawson, N.

AU - Zhang, Chi

AU - Foster, D.

AU - Li, Q.

AU - Nakatsugawa, M.

AU - Zajdel, R. W.

AU - Dube, D. K.

AU - Huang, X.

PY - 2001/6/6

Y1 - 2001/6/6

N2 - The Mexican axolotl (Ambystoma mexicanum) provides an excellent model for studying heart development since it carries a cardiac lethal mutation in gene c that results in failure of contraction of mutant embryonic myocardium. In cardiac mutant axolotls (c/c) the hearts do not beat, apparently because of an absence of organized myofibrils. To date, there has been no way to analyze the genotypes of embryos from heterozygous spawnings (+/c×+/c) until stage 35 when the normal (+/c or +/+) embryos first begin to have beating hearts; mutant (c/c) embryos fail to develop normal heartbeats. In the present study, we created chimeric axolotls by using microsurgical techniques. The general approach was to transect tailbud embryos and join the anterior and posterior halves of two different individuals. The chimeric axolotl is composed of a normal head and heart region (+/+), permitting survival and a mutant body containing mutant gonads (c/c) that permits the production of c/c mutant offspring: 100% c/c offspring were obtained by mating c/c chimeras (c/c×c/c). The mutant phenotypes were confirmed by the absence of beating hearts and death at stage 41 in 100% of the embryos. Examination of the mutant hearts with electron microscopy and confocal microscopy after immunofluorescent staining for tropomyosin showed identical images to those described previously in naturally-occurring c/c mutant axolotls (i.e., lacking organized sarcomeric myofibrils). These "c/c chimeric" axolotls provide a useful and unique way to investigate early embryonic heart development in cardiac mutant Mexican axolotls.

AB - The Mexican axolotl (Ambystoma mexicanum) provides an excellent model for studying heart development since it carries a cardiac lethal mutation in gene c that results in failure of contraction of mutant embryonic myocardium. In cardiac mutant axolotls (c/c) the hearts do not beat, apparently because of an absence of organized myofibrils. To date, there has been no way to analyze the genotypes of embryos from heterozygous spawnings (+/c×+/c) until stage 35 when the normal (+/c or +/+) embryos first begin to have beating hearts; mutant (c/c) embryos fail to develop normal heartbeats. In the present study, we created chimeric axolotls by using microsurgical techniques. The general approach was to transect tailbud embryos and join the anterior and posterior halves of two different individuals. The chimeric axolotl is composed of a normal head and heart region (+/+), permitting survival and a mutant body containing mutant gonads (c/c) that permits the production of c/c mutant offspring: 100% c/c offspring were obtained by mating c/c chimeras (c/c×c/c). The mutant phenotypes were confirmed by the absence of beating hearts and death at stage 41 in 100% of the embryos. Examination of the mutant hearts with electron microscopy and confocal microscopy after immunofluorescent staining for tropomyosin showed identical images to those described previously in naturally-occurring c/c mutant axolotls (i.e., lacking organized sarcomeric myofibrils). These "c/c chimeric" axolotls provide a useful and unique way to investigate early embryonic heart development in cardiac mutant Mexican axolotls.

KW - Animal

KW - Chimera

KW - Gene

KW - Mutation

KW - Myocardium

UR - http://www.scopus.com/inward/record.url?scp=0035001839&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035001839&partnerID=8YFLogxK

U2 - 10.1007/s004290100158

DO - 10.1007/s004290100158

M3 - Article

C2 - 11411308

AN - SCOPUS:0035001839

VL - 203

SP - 335

EP - 342

JO - Referate und Beiträge zur Anatomie und Entwickelungsgeschichte

JF - Referate und Beiträge zur Anatomie und Entwickelungsgeschichte

SN - 0177-5154

IS - 5

ER -