cpmA, a gene involved in an output pathway of the cyanobacterial circadian system

Mitsunori Katayama, Nicholas F. Tsinoremas, Takao Kondo, Susan S. Golden

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

We generated random mutations in Synechococcus sp. strain PCC 7942 to look for genes of output pathways in the cyanobacterial circadian system. A derivative of transposon Tn5 was introduced into the chromosomes of reporter strains in which cyanobacterial promoters drive the Vibrio harveyi luxAB genes and produce an oscillation of bioluminescence as a function of circadian gene expression. Among low-amplitude mutants, one mutant, tnp6, had an insertion in a 780-bp open reading frame. The tnp6 mutation produced an altered circadian phasing phenotype in the expression rhythms of psbAI::luxAB,psbAII::luxAB, and kaiA::luxAB but had no or little effect on those of psbAIII::luxAB, purF::luxAB, kaiB::luxAB, rpoD2::luxAB, ndhD::luxAB, and conII::luxAB. This suggests that the interrupted gene in tnp6, named cpmA (circadian phase modifier), is part of a circadian output pathway that regulates the expression rhythms of psbAI, psbAII, and kaiA.

Original languageEnglish (US)
Pages (from-to)3516-3524
Number of pages9
JournalJournal of bacteriology
Volume181
Issue number11
StatePublished - Jun 1 1999
Externally publishedYes

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ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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