cpmA, a gene involved in an output pathway of the cyanobacterial circadian system

Mitsunori Katayama, Nicholas Tsinoremas, Takao Kondo, Susan S. Golden

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

We generated random mutations in Synechococcus sp. strain PCC 7942 to look for genes of output pathways in the cyanobacterial circadian system. A derivative of transposon Tn5 was introduced into the chromosomes of reporter strains in which cyanobacterial promoters drive the Vibrio harveyi luxAB genes and produce an oscillation of bioluminescence as a function of circadian gene expression. Among low-amplitude mutants, one mutant, tnp6, had an insertion in a 780-bp open reading frame. The tnp6 mutation produced an altered circadian phasing phenotype in the expression rhythms of psbAI::luxAB,psbAII::luxAB, and kaiA::luxAB but had no or little effect on those of psbAIII::luxAB, purF::luxAB, kaiB::luxAB, rpoD2::luxAB, ndhD::luxAB, and conII::luxAB. This suggests that the interrupted gene in tnp6, named cpmA (circadian phase modifier), is part of a circadian output pathway that regulates the expression rhythms of psbAI, psbAII, and kaiA.

Original languageEnglish
Pages (from-to)3516-3524
Number of pages9
JournalJournal of Bacteriology
Volume181
Issue number11
StatePublished - Jun 1 1999
Externally publishedYes

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Genes
Synechococcus
Mutation
Vibrio
Open Reading Frames
Chromosomes
Phenotype
Gene Expression

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

cpmA, a gene involved in an output pathway of the cyanobacterial circadian system. / Katayama, Mitsunori; Tsinoremas, Nicholas; Kondo, Takao; Golden, Susan S.

In: Journal of Bacteriology, Vol. 181, No. 11, 01.06.1999, p. 3516-3524.

Research output: Contribution to journalArticle

Katayama, Mitsunori ; Tsinoremas, Nicholas ; Kondo, Takao ; Golden, Susan S. / cpmA, a gene involved in an output pathway of the cyanobacterial circadian system. In: Journal of Bacteriology. 1999 ; Vol. 181, No. 11. pp. 3516-3524.
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