CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA

Meir Shamay, Nicholas Hand, M. Victor Lemas, Henry B. Koon, Susan E. Krown, John Wrangle, Prashant Desai, Juan Carlos Ramos, Richard F. Ambinder

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Abstract

We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.

Original languageEnglish (US)
Pages (from-to)1095-1099
Number of pages5
JournalJournal of Infectious Diseases
Volume205
Issue number7
DOIs
StatePublished - Apr 1 2012

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Shamay, M., Hand, N., Lemas, M. V., Koon, H. B., Krown, S. E., Wrangle, J., Desai, P., Ramos, J. C., & Ambinder, R. F. (2012). CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA. Journal of Infectious Diseases, 205(7), 1095-1099. https://doi.org/10.1093/infdis/jis032