CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA

Meir Shamay, Nicholas Hand, M. Victor Lemas, Henry B. Koon, Susan E. Krown, John Wrangle, Prashant Desai, Juan Carlos Ramos, Richard F. Ambinder

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.

Original languageEnglish
Pages (from-to)1095-1099
Number of pages5
JournalJournal of Infectious Diseases
Volume205
Issue number7
DOIs
StatePublished - Apr 1 2012

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Kaposi's Sarcoma
Herpesviridae
Methylation
Primary Effusion Lymphoma
DNA
Virion
AIDS-Related Lymphoma
Cell Separation
Carrier Proteins
Neoplasms

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Shamay, M., Hand, N., Lemas, M. V., Koon, H. B., Krown, S. E., Wrangle, J., ... Ambinder, R. F. (2012). CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA. Journal of Infectious Diseases, 205(7), 1095-1099. https://doi.org/10.1093/infdis/jis032

CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA. / Shamay, Meir; Hand, Nicholas; Lemas, M. Victor; Koon, Henry B.; Krown, Susan E.; Wrangle, John; Desai, Prashant; Carlos Ramos, Juan; Ambinder, Richard F.

In: Journal of Infectious Diseases, Vol. 205, No. 7, 01.04.2012, p. 1095-1099.

Research output: Contribution to journalArticle

Shamay, M, Hand, N, Lemas, MV, Koon, HB, Krown, SE, Wrangle, J, Desai, P, Carlos Ramos, J & Ambinder, RF 2012, 'CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA', Journal of Infectious Diseases, vol. 205, no. 7, pp. 1095-1099. https://doi.org/10.1093/infdis/jis032
Shamay, Meir ; Hand, Nicholas ; Lemas, M. Victor ; Koon, Henry B. ; Krown, Susan E. ; Wrangle, John ; Desai, Prashant ; Carlos Ramos, Juan ; Ambinder, Richard F. / CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA. In: Journal of Infectious Diseases. 2012 ; Vol. 205, No. 7. pp. 1095-1099.
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