Coupling of [Ca2+]i and ciliary beating in cultured tracheal epithelial cells

Matthias A Salathe, Richard J. Bookman

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The molecular mechanisms responsible for the regulation of ciliary beating frequency (CBF) are only partially characterized. To determine whether elevation of intracellular Ca2+ ([Ca2+]i) can cause an increase in CBF, we measured CBF and Ca2+ in single cells. Ovine tracheal epithelial cells, obtained by dissociation with protease, were grown in primary culture for 1 to 28 days in a mucus-free system. CBF of a single cilium was measured by digital video phase-contrast microscopy and on-line Fourier-transform analysis. Changes in [Ca2+]i from single cells were determined with fura-2, using ratio imaging video microscopy. Activation of a muscarinic pathway with 10 μM ACh (acetylcholine) increased [Ca2+]i from 53±9 nM (mean ± s.e.m.) to 146±12 nM or to 264±51% above initial baseline. In the same cells, ACh increased CBF from a baseline of 7±0.5 Hz to 9±0.2 Hz or to 31±2.8% above baseline (n=14). The elevations of both [Ca2+]i and CBF were transient and relaxed back to an elevated plateau (10/14 cells) as long as ACh was present. To elevate [Ca2+]i by mechanisms independent of a G-protein-coupled receptor, we measured [Ca2+]i and CBF of the same cells in extracellular solutions with either 0 Ca2+ (+ 1 mM EGTA) or 10 mM Ca2+. Both signals rose and fell with similar kinetics in response to changing [Ca2+]0, suggesting that changes in [Ca2+]i alone can modulate CBF. In a second independent manipulation, cells were treated with 1 μM thapsigargin, an irreversible inhibitor of the endoplasmic reticulum Ca2+-ATPase. Upon thapsigargin addition, 37 of 42 cells showed a transient [Ca2+]i increase and, as measured in different experiments, 8 of 9 cells showed a transient increase in CBF. Interestingly, application of ACh after cells were treated with thapsigargin produced decreases in both [Ca2+]i and CBF in 8/8 cells. Lastly, after 1-3 days in culture, addition of 10 μM ACh often produced [Ca2+]i oscillations rather than transients in [Ca2+]i. Measurements of CBF in these cells showed frequency modulation of CBF with the same peak-to-peak time interval as the Ca2+ oscillation. These results show that: (1) CBF can be measured from a single cilium and monitored on-line to track changes; (2) CBF and [Ca2+]i can be measured in the same single cell; (3) transient changes in [Ca2+]i (induced by 4 different manipulations) are associated with kinetically similar changes in CBF; and (4) [Ca2+]i oscillations are coupled to frequency modulation of ciliary beating. Taken together, these results provide strong evidence that [Ca2+]i is a critical intracellular messenger in the regulation of CBF in mammalian tracheal epithelial cells.

Original languageEnglish
Pages (from-to)431-440
Number of pages10
JournalJournal of Cell Science
Volume108
Issue number2
StatePublished - Feb 1 1995

Fingerprint

Epithelial Cells
Acetylcholine
Thapsigargin
Video Microscopy
Cilia
Fourier Analysis
Phase-Contrast Microscopy
Calcium-Transporting ATPases
Fura-2
Egtazic Acid
Mucus
G-Protein-Coupled Receptors
Endoplasmic Reticulum
Cholinergic Agents
Sheep
Peptide Hydrolases

Keywords

  • Acetylcholine
  • Calcium
  • Cilium
  • Epithelium
  • Trachea
  • Video microscopy

ASJC Scopus subject areas

  • Cell Biology

Cite this

Coupling of [Ca2+]i and ciliary beating in cultured tracheal epithelial cells. / Salathe, Matthias A; Bookman, Richard J.

In: Journal of Cell Science, Vol. 108, No. 2, 01.02.1995, p. 431-440.

Research output: Contribution to journalArticle

Salathe, MA & Bookman, RJ 1995, 'Coupling of [Ca2+]i and ciliary beating in cultured tracheal epithelial cells', Journal of Cell Science, vol. 108, no. 2, pp. 431-440.
Salathe, Matthias A ; Bookman, Richard J. / Coupling of [Ca2+]i and ciliary beating in cultured tracheal epithelial cells. In: Journal of Cell Science. 1995 ; Vol. 108, No. 2. pp. 431-440.
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N2 - The molecular mechanisms responsible for the regulation of ciliary beating frequency (CBF) are only partially characterized. To determine whether elevation of intracellular Ca2+ ([Ca2+]i) can cause an increase in CBF, we measured CBF and Ca2+ in single cells. Ovine tracheal epithelial cells, obtained by dissociation with protease, were grown in primary culture for 1 to 28 days in a mucus-free system. CBF of a single cilium was measured by digital video phase-contrast microscopy and on-line Fourier-transform analysis. Changes in [Ca2+]i from single cells were determined with fura-2, using ratio imaging video microscopy. Activation of a muscarinic pathway with 10 μM ACh (acetylcholine) increased [Ca2+]i from 53±9 nM (mean ± s.e.m.) to 146±12 nM or to 264±51% above initial baseline. In the same cells, ACh increased CBF from a baseline of 7±0.5 Hz to 9±0.2 Hz or to 31±2.8% above baseline (n=14). The elevations of both [Ca2+]i and CBF were transient and relaxed back to an elevated plateau (10/14 cells) as long as ACh was present. To elevate [Ca2+]i by mechanisms independent of a G-protein-coupled receptor, we measured [Ca2+]i and CBF of the same cells in extracellular solutions with either 0 Ca2+ (+ 1 mM EGTA) or 10 mM Ca2+. Both signals rose and fell with similar kinetics in response to changing [Ca2+]0, suggesting that changes in [Ca2+]i alone can modulate CBF. In a second independent manipulation, cells were treated with 1 μM thapsigargin, an irreversible inhibitor of the endoplasmic reticulum Ca2+-ATPase. Upon thapsigargin addition, 37 of 42 cells showed a transient [Ca2+]i increase and, as measured in different experiments, 8 of 9 cells showed a transient increase in CBF. Interestingly, application of ACh after cells were treated with thapsigargin produced decreases in both [Ca2+]i and CBF in 8/8 cells. Lastly, after 1-3 days in culture, addition of 10 μM ACh often produced [Ca2+]i oscillations rather than transients in [Ca2+]i. Measurements of CBF in these cells showed frequency modulation of CBF with the same peak-to-peak time interval as the Ca2+ oscillation. These results show that: (1) CBF can be measured from a single cilium and monitored on-line to track changes; (2) CBF and [Ca2+]i can be measured in the same single cell; (3) transient changes in [Ca2+]i (induced by 4 different manipulations) are associated with kinetically similar changes in CBF; and (4) [Ca2+]i oscillations are coupled to frequency modulation of ciliary beating. Taken together, these results provide strong evidence that [Ca2+]i is a critical intracellular messenger in the regulation of CBF in mammalian tracheal epithelial cells.

AB - The molecular mechanisms responsible for the regulation of ciliary beating frequency (CBF) are only partially characterized. To determine whether elevation of intracellular Ca2+ ([Ca2+]i) can cause an increase in CBF, we measured CBF and Ca2+ in single cells. Ovine tracheal epithelial cells, obtained by dissociation with protease, were grown in primary culture for 1 to 28 days in a mucus-free system. CBF of a single cilium was measured by digital video phase-contrast microscopy and on-line Fourier-transform analysis. Changes in [Ca2+]i from single cells were determined with fura-2, using ratio imaging video microscopy. Activation of a muscarinic pathway with 10 μM ACh (acetylcholine) increased [Ca2+]i from 53±9 nM (mean ± s.e.m.) to 146±12 nM or to 264±51% above initial baseline. In the same cells, ACh increased CBF from a baseline of 7±0.5 Hz to 9±0.2 Hz or to 31±2.8% above baseline (n=14). The elevations of both [Ca2+]i and CBF were transient and relaxed back to an elevated plateau (10/14 cells) as long as ACh was present. To elevate [Ca2+]i by mechanisms independent of a G-protein-coupled receptor, we measured [Ca2+]i and CBF of the same cells in extracellular solutions with either 0 Ca2+ (+ 1 mM EGTA) or 10 mM Ca2+. Both signals rose and fell with similar kinetics in response to changing [Ca2+]0, suggesting that changes in [Ca2+]i alone can modulate CBF. In a second independent manipulation, cells were treated with 1 μM thapsigargin, an irreversible inhibitor of the endoplasmic reticulum Ca2+-ATPase. Upon thapsigargin addition, 37 of 42 cells showed a transient [Ca2+]i increase and, as measured in different experiments, 8 of 9 cells showed a transient increase in CBF. Interestingly, application of ACh after cells were treated with thapsigargin produced decreases in both [Ca2+]i and CBF in 8/8 cells. Lastly, after 1-3 days in culture, addition of 10 μM ACh often produced [Ca2+]i oscillations rather than transients in [Ca2+]i. Measurements of CBF in these cells showed frequency modulation of CBF with the same peak-to-peak time interval as the Ca2+ oscillation. These results show that: (1) CBF can be measured from a single cilium and monitored on-line to track changes; (2) CBF and [Ca2+]i can be measured in the same single cell; (3) transient changes in [Ca2+]i (induced by 4 different manipulations) are associated with kinetically similar changes in CBF; and (4) [Ca2+]i oscillations are coupled to frequency modulation of ciliary beating. Taken together, these results provide strong evidence that [Ca2+]i is a critical intracellular messenger in the regulation of CBF in mammalian tracheal epithelial cells.

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