Coupling biomolecules to fullerenes through a molecular adapter

Marcello Capaccio, Vasilis G. Gavalas, Mark S. Meier, John E. Anthony, Leonidas G. Bachas

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

A novel biotinylated fullerene has been synthesized to facilitate the attachment of biotin-conjugated proteins to C60 through the use of streptavidin as a molecular adapter. The strong biotin-streptavidin interaction enables the attachment of fullerenes to streptavidin and, because of the availability of four biotin-binding sites on streptavidin, to biotinylated biomolecules. The feasibility of this approach is demonstrated by using biotinylated alkaline phosphatase. Due to the insolubility of fullerenes in aqueous media, the immobilized enzyme can be eventually recovered by simple centrifugation with no significant loss in activity.

Original languageEnglish (US)
Pages (from-to)241-244
Number of pages4
JournalBioconjugate Chemistry
Volume16
Issue number2
DOIs
StatePublished - Mar 1 2005
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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