Coupling biomolecules to fullerenes through a molecular adapter

Marcello Capaccio, Vasilis G. Gavalas, Mark S. Meier, John E. Anthony, Leonidas G Bachas

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A novel biotinylated fullerene has been synthesized to facilitate the attachment of biotin-conjugated proteins to C60 through the use of streptavidin as a molecular adapter. The strong biotin-streptavidin interaction enables the attachment of fullerenes to streptavidin and, because of the availability of four biotin-binding sites on streptavidin, to biotinylated biomolecules. The feasibility of this approach is demonstrated by using biotinylated alkaline phosphatase. Due to the insolubility of fullerenes in aqueous media, the immobilized enzyme can be eventually recovered by simple centrifugation with no significant loss in activity.

Original languageEnglish (US)
Pages (from-to)241-244
Number of pages4
JournalBioconjugate Chemistry
Volume16
Issue number2
DOIs
StatePublished - Mar 2005
Externally publishedYes

Fingerprint

Fullerenes
Streptavidin
Biomolecules
Biotin
Centrifugation
Phosphatases
Binding sites
Immobilized Enzymes
Enzymes
Solubility
Availability
Proteins
Alkaline Phosphatase
Binding Sites

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Clinical Biochemistry
  • Chemistry(all)
  • Organic Chemistry

Cite this

Coupling biomolecules to fullerenes through a molecular adapter. / Capaccio, Marcello; Gavalas, Vasilis G.; Meier, Mark S.; Anthony, John E.; Bachas, Leonidas G.

In: Bioconjugate Chemistry, Vol. 16, No. 2, 03.2005, p. 241-244.

Research output: Contribution to journalArticle

Capaccio, M, Gavalas, VG, Meier, MS, Anthony, JE & Bachas, LG 2005, 'Coupling biomolecules to fullerenes through a molecular adapter', Bioconjugate Chemistry, vol. 16, no. 2, pp. 241-244. https://doi.org/10.1021/bc049861d
Capaccio, Marcello ; Gavalas, Vasilis G. ; Meier, Mark S. ; Anthony, John E. ; Bachas, Leonidas G. / Coupling biomolecules to fullerenes through a molecular adapter. In: Bioconjugate Chemistry. 2005 ; Vol. 16, No. 2. pp. 241-244.
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