Coupled and uncoupled proton movement by amino acid transport system N

Farrukh A. Chaudhry, David Krizaj, Peter Larsson, Richard J. Reimer, Christopher Wreden, Jon Storm-Mathisen, David Copenhagen, Michael Kavanaugh, Robert H. Edwards

Research output: Contribution to journalArticle

89 Scopus citations

Abstract

The system N transporter SN1 has been proposed to mediate the efflux of glutamine from cells required to sustain the urea cycle and the glutamine-glutamate cycle that regenerates glutamate and γ-aminobutyric acid (GABA) for synaptic release. We now show that SN1 also mediates an ionic conductance activated by glutamine, and this conductance is selective for H+. Although SN1 couples amino acid uptake to H+ exchange, the glutamine-gated H+ conductance is not stoichiometrically coupled to transport. Protons thus permeate SN1 both coupled to and uncoupled from amino acid flux, providing novel mechanisms to regulate the transfer of glutamine between cells.

Original languageEnglish (US)
Pages (from-to)7041-7051
Number of pages11
JournalEMBO Journal
Volume20
Issue number24
DOIs
StatePublished - Dec 17 2001

Keywords

  • Amino acid tranport system N
  • Asparagine
  • Glutamine
  • H conductance
  • SN1

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Coupled and uncoupled proton movement by amino acid transport system N'. Together they form a unique fingerprint.

  • Cite this

    Chaudhry, F. A., Krizaj, D., Larsson, P., Reimer, R. J., Wreden, C., Storm-Mathisen, J., Copenhagen, D., Kavanaugh, M., & Edwards, R. H. (2001). Coupled and uncoupled proton movement by amino acid transport system N. EMBO Journal, 20(24), 7041-7051. https://doi.org/10.1093/emboj/20.24.7041