CO2 hydration by immobilized carbonic anhydrase

Sumana Bhattacharya, Marc Schiavone, Subhra Chakrabarti, Sanjoy K Bhattacharya

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The enzyme carbonic anhydrase (isoform II) from bovine and human erythrocytes was immobilized using different covalent coupling methods on inert matrices. Immobilized carbonic anhydrase may enable concentration of CO 2 for Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)- catalysed fixation in bioreactors. In the present study the activity of carbonic anhydrase with respect to hydration of CO2 using soluble and immobilized enzymes was determined. The stability of the immobilization matrix, the properties of the immobilized enzymes subjected to a variation in operation variables and the activity profile with respect to storage are reported. Immobilization imparted greater thermal and storage stability and enhanced reusability.

Original languageEnglish
Pages (from-to)111-117
Number of pages7
JournalBiotechnology and Applied Biochemistry
Volume38
Issue number2
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

Fingerprint

Carbonic anhydrase
Immobilized Enzymes
Carbonic Anhydrases
Immobilization
Hydration
Enzymes
Carbonic Anhydrase II
Oxygenases
Bioreactors
Carbon Monoxide
Protein Isoforms
Hot Temperature
Erythrocytes
Reusability
ribulose-1,5 diphosphate

Keywords

  • Carbonic anhydrase
  • CO concentration
  • CO-fixation bioprocess
  • Immobilized carbonic anhydrase
  • Immobilized carbonic anhydrase bioreactor

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Cite this

CO2 hydration by immobilized carbonic anhydrase. / Bhattacharya, Sumana; Schiavone, Marc; Chakrabarti, Subhra; Bhattacharya, Sanjoy K.

In: Biotechnology and Applied Biochemistry, Vol. 38, No. 2, 01.10.2003, p. 111-117.

Research output: Contribution to journalArticle

Bhattacharya, Sumana ; Schiavone, Marc ; Chakrabarti, Subhra ; Bhattacharya, Sanjoy K. / CO2 hydration by immobilized carbonic anhydrase. In: Biotechnology and Applied Biochemistry. 2003 ; Vol. 38, No. 2. pp. 111-117.
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