Costal2 Functions as a Kinesin-like Protein in the Hedgehog Signal Transduction Pathway

Shohreh F. Farzan, Manuel Ascano, Stacey K. Ogden, Matthieu Sanial, Amira Brigui, Anne Plessis, David J Robbins

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The Hedgehog (Hh) signaling pathway initiates an evolutionarily conserved developmental program required for the proper patterning of many tissues [1]. Although Costal2 (Cos2) is a requisite component of the Hh pathway, its mechanistic role is not well understood. Because of its primary sequence, Cos2 was initially predicted to function as a kinesin-like protein [2]. However, evidence showing that Cos2 function might require kinesin-like properties has been lacking [2-6]. Thus, the prevailing dogma in the field is that Cos2 functions solely as a scaffolding protein [7, 8]. Here, we show that Cos2 motility is required for its biological function and that this motility may be Hh regulated. We show that Cos2 motility requires an active motor domain, ATP, and microtubules. Additionally, Cos2 recruits and transports other components of the Hh signaling pathway, including the transcription factor Cubitus interruptus (Ci). Drosophila expressing cos2 mutations that encode proteins that lack motility are attenuated in their ability to regulate Ci activity and exhibit phenotypes consistent with attenuated Cos2 function [9]. Combined, these results demonstrate that Cos2 motility plays an important role in its function, regulating the amounts and activity of Ci that ultimately interpret the level of Hh to which cells are exposed.

Original languageEnglish
Pages (from-to)1215-1220
Number of pages6
JournalCurrent Biology
Volume18
Issue number16
DOIs
StatePublished - Aug 26 2008
Externally publishedYes

Fingerprint

Hedgehog Proteins
Kinesin
kinesin
Signal transduction
Hedgehogs
Erinaceidae
signal transduction
Signal Transduction
Proteins
proteins
molecular motor proteins
Molecular Motor Proteins
scaffolding proteins
Microtubules
microtubules
Drosophila
Transcription Factors
transcription factors
Adenosine Triphosphate
Tissue

Keywords

  • CELLBIO
  • SIGNALING

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Farzan, S. F., Ascano, M., Ogden, S. K., Sanial, M., Brigui, A., Plessis, A., & Robbins, D. J. (2008). Costal2 Functions as a Kinesin-like Protein in the Hedgehog Signal Transduction Pathway. Current Biology, 18(16), 1215-1220. https://doi.org/10.1016/j.cub.2008.07.026

Costal2 Functions as a Kinesin-like Protein in the Hedgehog Signal Transduction Pathway. / Farzan, Shohreh F.; Ascano, Manuel; Ogden, Stacey K.; Sanial, Matthieu; Brigui, Amira; Plessis, Anne; Robbins, David J.

In: Current Biology, Vol. 18, No. 16, 26.08.2008, p. 1215-1220.

Research output: Contribution to journalArticle

Farzan, SF, Ascano, M, Ogden, SK, Sanial, M, Brigui, A, Plessis, A & Robbins, DJ 2008, 'Costal2 Functions as a Kinesin-like Protein in the Hedgehog Signal Transduction Pathway', Current Biology, vol. 18, no. 16, pp. 1215-1220. https://doi.org/10.1016/j.cub.2008.07.026
Farzan SF, Ascano M, Ogden SK, Sanial M, Brigui A, Plessis A et al. Costal2 Functions as a Kinesin-like Protein in the Hedgehog Signal Transduction Pathway. Current Biology. 2008 Aug 26;18(16):1215-1220. https://doi.org/10.1016/j.cub.2008.07.026
Farzan, Shohreh F. ; Ascano, Manuel ; Ogden, Stacey K. ; Sanial, Matthieu ; Brigui, Amira ; Plessis, Anne ; Robbins, David J. / Costal2 Functions as a Kinesin-like Protein in the Hedgehog Signal Transduction Pathway. In: Current Biology. 2008 ; Vol. 18, No. 16. pp. 1215-1220.
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