Cortisol reduces paracellular permeability and increases occludin abundance in cultured trout gill epithelia

Helen Chasiotis, Chris M. Wood, Scott P. Kelly

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A role for the tight junction (TJ) protein occludin in the regulation of gill paracellular permeability was investigated using primary cultured " reconstructed" freshwater (FW) rainbow trout gill epithelia composed solely of pavement cells. Cortisol treatment reduced epithelial permeability characteristics, measured as changes in transepithelial resistance (TER) and paracellular [3H]PEG-4000 flux. Cortisol also reduced net Na+ flux rates when epithelia were exposed to apical FW. cDNA encoding for the TJ protein occludin was cloned from rainbow trout and found to be particularly abundant in gill tissue. In cultured gill preparations, occludin immunolocalized to the TJ complex and transcript abundance dose-dependently increased in response to cortisol treatment in association with reduced paracellular permeability. Occludin protein abundance also increased in response to cortisol treatment. However, occludin mRNA levels did not change in response to apical FW exposure, and [3H]PEG-4000 permeability did not decrease. These data support a role for occludin in the endocrine regulation of paracellular permeability across gill epithelia of fishes.

Original languageEnglish
Pages (from-to)232-238
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume323
Issue number2
DOIs
StatePublished - Jul 1 2010

Fingerprint

Occludin
Trout
Hydrocortisone
Permeability
Epithelium
Fresh Water
Tight Junction Proteins
Oncorhynchus mykiss
Polyethylene glycols
Fluxes
Tight Junctions
Pavements
Fish
Fishes
Complementary DNA
Tissue
Messenger RNA

Keywords

  • Ion transport
  • Osmoregulation
  • Pavement cells
  • Polyethylene glycol
  • Tight junction
  • Transepithelial resistance

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Cite this

Cortisol reduces paracellular permeability and increases occludin abundance in cultured trout gill epithelia. / Chasiotis, Helen; Wood, Chris M.; Kelly, Scott P.

In: Molecular and Cellular Endocrinology, Vol. 323, No. 2, 01.07.2010, p. 232-238.

Research output: Contribution to journalArticle

Chasiotis, Helen ; Wood, Chris M. ; Kelly, Scott P. / Cortisol reduces paracellular permeability and increases occludin abundance in cultured trout gill epithelia. In: Molecular and Cellular Endocrinology. 2010 ; Vol. 323, No. 2. pp. 232-238.
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