Corticotropin-releasing factor is secreted in the BE(2)-C neuroblastoma cell and is responsive to forskolin

John W. Kasckow, Michael J. Owens, Mark D. Stipetic, Han Jin-Hee Han, Charles B. Nemeroff

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The BE(2)-C human neuroblastoma has been shown to express corticotropin-releasing factor (CRF) messenger RNA. In this study, BE(2)-C cells were treated 5 days with 5 μM retinoic acid. Cell extracts were also applied onto a C18 Vydac column and fractions were assayed for CRF-like immunoreactivity (CRF-LI) which coincided in time of elution with oxidized or non-oxidized CRF synthetic CRF standard. With forskolin treatment, secretion media and cell extract CRF-LI increased in a concentration-dependent fashion. Thus, this cell line synthesizes and secretes CRF and is a good model for studying CRF regulation.

Original languageEnglish (US)
Pages (from-to)1825-1829
Number of pages5
JournalLife Sciences
Volume54
Issue number23
DOIs
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Fingerprint Dive into the research topics of 'Corticotropin-releasing factor is secreted in the BE(2)-C neuroblastoma cell and is responsive to forskolin'. Together they form a unique fingerprint.

  • Cite this