Abstract
The BE(2)-C human neuroblastoma has been shown to express corticotropin-releasing factor (CRF) messenger RNA. In this study, BE(2)-C cells were treated 5 days with 5 μM retinoic acid. Cell extracts were also applied onto a C18 Vydac column and fractions were assayed for CRF-like immunoreactivity (CRF-LI) which coincided in time of elution with oxidized or non-oxidized CRF synthetic CRF standard. With forskolin treatment, secretion media and cell extract CRF-LI increased in a concentration-dependent fashion. Thus, this cell line synthesizes and secretes CRF and is a good model for studying CRF regulation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1825-1829 |
| Number of pages | 5 |
| Journal | Life Sciences |
| Volume | 54 |
| Issue number | 23 |
| DOIs | |
| State | Published - 1994 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)
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Cite this
Kasckow, J. W., Owens, M. J., Stipetic, M. D., Jin-Hee Han, H., & Nemeroff, C. B. (1994). Corticotropin-releasing factor is secreted in the BE(2)-C neuroblastoma cell and is responsive to forskolin. Life Sciences, 54(23), 1825-1829. https://doi.org/10.1016/0024-3205(94)90121-X