Corticotropin-releasing factor is secreted in the BE(2)-C neuroblastoma cell and is responsive to forskolin

John W. Kasckow; Michael J. Owens; Mark D. Stipetic; Han Jin-Hee Han; Charles B. Nemeroff

doi:10.1016/0024-3205(94)90121-X

Corticotropin-releasing factor is secreted in the BE(2)-C neuroblastoma cell and is responsive to forskolin

John W. Kasckow, Michael J. Owens, Mark D. Stipetic, Han Jin-Hee Han, Charles B. Nemeroff

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The BE(2)-C human neuroblastoma has been shown to express corticotropin-releasing factor (CRF) messenger RNA. In this study, BE(2)-C cells were treated 5 days with 5 μM retinoic acid. Cell extracts were also applied onto a C18 Vydac column and fractions were assayed for CRF-like immunoreactivity (CRF-LI) which coincided in time of elution with oxidized or non-oxidized CRF synthetic CRF standard. With forskolin treatment, secretion media and cell extract CRF-LI increased in a concentration-dependent fashion. Thus, this cell line synthesizes and secretes CRF and is a good model for studying CRF regulation.

Original language	English (US)
Pages (from-to)	1825-1829
Number of pages	5
Journal	Life Sciences
Volume	54
Issue number	23
DOIs	https://doi.org/10.1016/0024-3205(94)90121-X
State	Published - 1994
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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@article{dd814d21bf7a465d920a0f55c2893820,

title = "Corticotropin-releasing factor is secreted in the BE(2)-C neuroblastoma cell and is responsive to forskolin",

abstract = "The BE(2)-C human neuroblastoma has been shown to express corticotropin-releasing factor (CRF) messenger RNA. In this study, BE(2)-C cells were treated 5 days with 5 μM retinoic acid. Cell extracts were also applied onto a C18 Vydac column and fractions were assayed for CRF-like immunoreactivity (CRF-LI) which coincided in time of elution with oxidized or non-oxidized CRF synthetic CRF standard. With forskolin treatment, secretion media and cell extract CRF-LI increased in a concentration-dependent fashion. Thus, this cell line synthesizes and secretes CRF and is a good model for studying CRF regulation.",

author = "Kasckow, {John W.} and Owens, {Michael J.} and Stipetic, {Mark D.} and {Jin-Hee Han}, Han and Nemeroff, {Charles B.}",

note = "Funding Information: Supported by NIMH MH 42088. Dr. Kasckow was supported by a Veterans Administration Psychiatry Research fellowship and the work was also performed while Dr. Kasckow was a Pfizer awardee in Biological Psychiatry.",

year = "1994",

doi = "10.1016/0024-3205(94)90121-X",

language = "English (US)",

volume = "54",

pages = "1825--1829",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "23",

}

TY - JOUR

T1 - Corticotropin-releasing factor is secreted in the BE(2)-C neuroblastoma cell and is responsive to forskolin

AU - Kasckow, John W.

AU - Owens, Michael J.

AU - Stipetic, Mark D.

AU - Jin-Hee Han, Han

AU - Nemeroff, Charles B.

N1 - Funding Information: Supported by NIMH MH 42088. Dr. Kasckow was supported by a Veterans Administration Psychiatry Research fellowship and the work was also performed while Dr. Kasckow was a Pfizer awardee in Biological Psychiatry.

PY - 1994

Y1 - 1994

N2 - The BE(2)-C human neuroblastoma has been shown to express corticotropin-releasing factor (CRF) messenger RNA. In this study, BE(2)-C cells were treated 5 days with 5 μM retinoic acid. Cell extracts were also applied onto a C18 Vydac column and fractions were assayed for CRF-like immunoreactivity (CRF-LI) which coincided in time of elution with oxidized or non-oxidized CRF synthetic CRF standard. With forskolin treatment, secretion media and cell extract CRF-LI increased in a concentration-dependent fashion. Thus, this cell line synthesizes and secretes CRF and is a good model for studying CRF regulation.

AB - The BE(2)-C human neuroblastoma has been shown to express corticotropin-releasing factor (CRF) messenger RNA. In this study, BE(2)-C cells were treated 5 days with 5 μM retinoic acid. Cell extracts were also applied onto a C18 Vydac column and fractions were assayed for CRF-like immunoreactivity (CRF-LI) which coincided in time of elution with oxidized or non-oxidized CRF synthetic CRF standard. With forskolin treatment, secretion media and cell extract CRF-LI increased in a concentration-dependent fashion. Thus, this cell line synthesizes and secretes CRF and is a good model for studying CRF regulation.

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DO - 10.1016/0024-3205(94)90121-X

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SP - 1825

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JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

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