TY - JOUR
T1 - Cortical reorganization following fertilization of sea urchin eggs
T2 - Sensitivity to cytochalasin B
AU - Banzhaf, William C.
AU - Warren, Robert H.
AU - McClay, David R.
PY - 1980/12
Y1 - 1980/12
N2 - A transient sensitivity to cytochalasin B was detected in freshly fertilized sea urchin eggs. Large membrane-limited protrusions formed when cytochalasin B was added between 1 and 6 min postinsemination. When cytochalasin B was removed from the cultures, eggs continued to develop with protrusions remaining, and cleavage furrows formed through many of the protrusions. Electron microscopy showed that cytochalasin B disrupted the microfilamentous structures that formed in the egg cortex after fertilization. Protrusions also appeared when eggs were activated by the Ca2+ ionophore A23187 followed by cytochalasin B addition. Protrusions did not appear when cytochalasin B was added at any time prior to fertilization, during insemination, or after the 6-min period postinsemination. Mechanical compression or incubation in a cortical isolation medium were effective as treatments enhance the cytochalasin B-sensitive response. These results suggest a time frame for postfertilization changes in the cortex of the zygote.
AB - A transient sensitivity to cytochalasin B was detected in freshly fertilized sea urchin eggs. Large membrane-limited protrusions formed when cytochalasin B was added between 1 and 6 min postinsemination. When cytochalasin B was removed from the cultures, eggs continued to develop with protrusions remaining, and cleavage furrows formed through many of the protrusions. Electron microscopy showed that cytochalasin B disrupted the microfilamentous structures that formed in the egg cortex after fertilization. Protrusions also appeared when eggs were activated by the Ca2+ ionophore A23187 followed by cytochalasin B addition. Protrusions did not appear when cytochalasin B was added at any time prior to fertilization, during insemination, or after the 6-min period postinsemination. Mechanical compression or incubation in a cortical isolation medium were effective as treatments enhance the cytochalasin B-sensitive response. These results suggest a time frame for postfertilization changes in the cortex of the zygote.
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U2 - 10.1016/0012-1606(80)90424-8
DO - 10.1016/0012-1606(80)90424-8
M3 - Article
C2 - 6778750
AN - SCOPUS:0019185371
VL - 80
SP - 506
EP - 515
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -