Cortical reorganization following fertilization of sea urchin eggs: Sensitivity to cytochalasin B

William C. Banzhaf, Robert H. Warren, David R. McClay

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A transient sensitivity to cytochalasin B was detected in freshly fertilized sea urchin eggs. Large membrane-limited protrusions formed when cytochalasin B was added between 1 and 6 min postinsemination. When cytochalasin B was removed from the cultures, eggs continued to develop with protrusions remaining, and cleavage furrows formed through many of the protrusions. Electron microscopy showed that cytochalasin B disrupted the microfilamentous structures that formed in the egg cortex after fertilization. Protrusions also appeared when eggs were activated by the Ca2+ ionophore A23187 followed by cytochalasin B addition. Protrusions did not appear when cytochalasin B was added at any time prior to fertilization, during insemination, or after the 6-min period postinsemination. Mechanical compression or incubation in a cortical isolation medium were effective as treatments enhance the cytochalasin B-sensitive response. These results suggest a time frame for postfertilization changes in the cortex of the zygote.

Original languageEnglish (US)
Pages (from-to)506-515
Number of pages10
JournalDevelopmental Biology
Volume80
Issue number2
DOIs
StatePublished - Dec 1980

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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