Correlation of the extracellular glutamate concentration with extent of blood flow reduction after subdural hematoma in the rat

Ross Bullock, S. P. Butcher, M. H. Chen, L. Kendall, J. McCulloch

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

The excitatory neurotransmitters glutamate and aspartate are an important factor in the causation of ischemic brain damage. The concentration of glutamate and aspartate was serially measured in extracellular fluid using in vivo microdialysis after induction of a subdural hematoma or after a sham operation in the rat. Measurements were made in the cortex underlying the hematoma and in the ipsilateral hippocampus, and these findings were correlated with regional cerebral blood flow (CBF), measured autoradiographically 2 hours after hematoma induction. In the severely ischemic cortex underlying the hematoma (mean CBF < 25 ml/100 gm/min), glutamate and aspartate content increased more than 750% over basal levels. In individual animals the magnitude of glutamate release correlated with the extent of the focal ischemic zone under the hematoma (r = 0.907). Hippocampal glutamate levels rose 339%, yet regional CBF was preserved (114 ml/100 gm/min). This accords with focal hypermetabolism in this model, and may imply a glutamate-mediated 'excitotoxic' process after subdural hematoma.

Original languageEnglish
Pages (from-to)794-802
Number of pages9
JournalJournal of Neurosurgery
Volume74
Issue number5
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Subdural Hematoma
Glutamic Acid
Cerebrovascular Circulation
Hematoma
Aspartic Acid
Regional Blood Flow
Extracellular Fluid
Microdialysis
Causality
Neurotransmitter Agents
Hippocampus
Brain

Keywords

  • brain ischemia
  • dialysis
  • excitotoxicity
  • glutamate
  • microdialysis
  • rat
  • subdural hematoma

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Correlation of the extracellular glutamate concentration with extent of blood flow reduction after subdural hematoma in the rat. / Bullock, Ross; Butcher, S. P.; Chen, M. H.; Kendall, L.; McCulloch, J.

In: Journal of Neurosurgery, Vol. 74, No. 5, 01.01.1991, p. 794-802.

Research output: Contribution to journalArticle

Bullock, R, Butcher, SP, Chen, MH, Kendall, L & McCulloch, J 1991, 'Correlation of the extracellular glutamate concentration with extent of blood flow reduction after subdural hematoma in the rat', Journal of Neurosurgery, vol. 74, no. 5, pp. 794-802.
Bullock, Ross ; Butcher, S. P. ; Chen, M. H. ; Kendall, L. ; McCulloch, J. / Correlation of the extracellular glutamate concentration with extent of blood flow reduction after subdural hematoma in the rat. In: Journal of Neurosurgery. 1991 ; Vol. 74, No. 5. pp. 794-802.
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