Correlation of in vitro clonogenic assay data with in vivo growth delays and cell cycle changes of a human melanoma xenograft

V. Gupta, A. Krishan, C. G. Zubrod

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have reported previously that, in a human melanoma xenograft line, plating efficiency and drug sensitivity in vitro were enhanced under hypoxic conditions. In the present study, we show that the in vitro chemosensitivity data using clonogenic assays at 5% oxygen also correlate better with the drug-induced in vivo growth delay. Tumors implanted in bilaterally symmetrical locations grew at different rates, and response to chemotherapy was also variable. Cell cycle changes in the xenograft, as monitored by flow cytometry, suggest that major changes in cell cycle traverse occur when a drug is active in vitro and also causes in vivo growth delay. These studies indicate the usefulness of the nude mouse human xenografts to study correlations between soft agar clonogenic assays, in vivo growth delay, and cell cycle traverse in response to chemotherapeutic manipulation.

Original languageEnglish
Pages (from-to)2560-2564
Number of pages5
JournalCancer Research
Volume43
Issue number6
StatePublished - Jan 1 1983
Externally publishedYes

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Heterografts
Melanoma
Cell Cycle
Growth
Pharmaceutical Preparations
Nude Mice
Agar
Flow Cytometry
Oxygen
Drug Therapy
In Vitro Techniques
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Correlation of in vitro clonogenic assay data with in vivo growth delays and cell cycle changes of a human melanoma xenograft. / Gupta, V.; Krishan, A.; Zubrod, C. G.

In: Cancer Research, Vol. 43, No. 6, 01.01.1983, p. 2560-2564.

Research output: Contribution to journalArticle

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