Correlation of a unique 220-kDa protein with vitamin D sensitivity in glioma cells

Jiangying Zou, Howard Landy, Lynn G Feun, Rong Xu, Theodore Lampidis, Chun Jing Wu, A. J. Furst, Niramol Savaraj

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

We have investigated the antitumor and apoptotic effects of 1,25- dihydroxyvitamin D3 (VD3) in glioma cell lines and in primary cultures derived from surgical specimens from patients. Our results showed that certain glioma cells underwent apoptosis, whereas others were resistant. In an attempt to search for parameters that dictate VD3 sensitivity, we discovered a unique 220-kDa protein in glioma cells that were sensitive to VD3. This protein was not a classical vitamin D receptor (VDR), but was recognized by two different anti-VDR monoclonal antibodies. Furthermore, the level of the 220-kDa protein was inversely correlated with the IC50 of VD3 in these glioma cells. This 220-kDa protein was also present in frozen brain tumor samples, and the level of expression appeared to correlate with their corresponding primary cultures. Thus, our findings suggest that this 220-kDa protein may play an important role in determining VD3 sensitivity in malignant glioma. (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)1361-1365
Number of pages5
JournalBiochemical Pharmacology
Volume60
Issue number9
DOIs
StatePublished - Nov 1 2000

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Keywords

  • Apoptosis
  • Brain tumor
  • Malignant glioma
  • Primary culture
  • Vitamin D
  • Vitamin D receptor

ASJC Scopus subject areas

  • Pharmacology

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