Correlation between extent of liver damage in fulminant hepatic necrosis and complexing of circulating group-specific component (vitamin D-binding protein)

William O. Young, Pascal J. Goldschmidt-Clermont, David L. Emerson, William M. Lee, David J. Jollow, Robert M. Galbraith

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Increased complexing of circulating group-specific component (Gc, vitamin D-binding protein) has been found in humans under conditions of presumed increased actin release, and particularly fulminant hepatic necrosis (FHN). We therefore used a hamster model of acetaminophen-induced FHN to further investigate this phenomenon. Liver damage was monitored by aspartate aminotransferase (AST) activity and by histologic examination, and serum Gc was analyzed by polyacrylamide gel electrophoresis (PAGE) and transbloting with anti-Gc. In controls and treated animals displaying slight liver damage, >90% of Gc was of mobility corresponding to native purified hamster Gc, whereas with more severe liver damage up to 100% of Gc was found in one of two cathodal configurations that appear to correspond to complexes with actin. Densitometric quantitation of complexed Gc demonstrated a strong correlation with the severity of liver damage. These results show PAGE to be a useful method of estimating the percentage of Gc complexed in serum, and suggest that cell damage may be followed by the appearance in the circulation of cellular actin that complexes with Gc.

Original languageEnglish (US)
Pages (from-to)83-90
Number of pages8
JournalThe Journal of Laboratory and Clinical Medicine
Volume110
Issue number1
StatePublished - Jul 1987

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Correlation between extent of liver damage in fulminant hepatic necrosis and complexing of circulating group-specific component (vitamin D-binding protein)'. Together they form a unique fingerprint.

Cite this