Correlation between amino acid release and neuropathologic outcome in rat brain following middle cerebral artery occlusion

Steven P. Butcher, Ross Bullock, David I. Graham, James McCulloch

Research output: Contribution to journalArticle

244 Citations (Scopus)

Abstract

Using in vivo brain microdialysis, we studied amino acid release in the striatum and cortex of eight rats following permanent middle cerebral artery occlusion. We then processed all brains for histopathologic assessment of the volume of ischemic damage 4 hours after occlusion. Ischemic damage was varied by occlusion of the middle cerebral artery at a point either proximal (n=4) or distal (n=4) to the lenticulostriate vessels. Proximal occlusion elevated the dialysate contents of all amino acids. The largest increases occurred for the potentially neurotoxic amino acids aspartate and glutamate and for taurine (800-2,800% of basal efflux). We observed smaller increases for the "metabolic" amino acids (280-580% of basal efflux). Distal occlusion did not affect amino acid efflux in the striatum, and release in the cortex was significantly lower than that following proximal occlusion. We compared release data with acute histopathologic outcome. Proximal occlusion resulted in a large volume of ischemic damage in the cortex and striatum (25-48% of hemispheric volume). A smaller volume of ischemic damage was noted following distal occlusion (0-21% of hemispheric volume). The volume of ischemic damage and the amount of amino acid release were significantly correlated (p<0.05).

Original languageEnglish
Pages (from-to)1727-1733
Number of pages7
JournalStroke
Volume21
Issue number12
StatePublished - Dec 1 1990
Externally publishedYes

Fingerprint

Middle Cerebral Artery Infarction
Amino Acids
Brain
Taurine
Dialysis Solutions
Microdialysis
Aspartic Acid
Glutamic Acid

Keywords

  • Amino acids
  • Glutamates
  • Rats

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Neuroscience(all)

Cite this

Butcher, S. P., Bullock, R., Graham, D. I., & McCulloch, J. (1990). Correlation between amino acid release and neuropathologic outcome in rat brain following middle cerebral artery occlusion. Stroke, 21(12), 1727-1733.

Correlation between amino acid release and neuropathologic outcome in rat brain following middle cerebral artery occlusion. / Butcher, Steven P.; Bullock, Ross; Graham, David I.; McCulloch, James.

In: Stroke, Vol. 21, No. 12, 01.12.1990, p. 1727-1733.

Research output: Contribution to journalArticle

Butcher, SP, Bullock, R, Graham, DI & McCulloch, J 1990, 'Correlation between amino acid release and neuropathologic outcome in rat brain following middle cerebral artery occlusion', Stroke, vol. 21, no. 12, pp. 1727-1733.
Butcher, Steven P. ; Bullock, Ross ; Graham, David I. ; McCulloch, James. / Correlation between amino acid release and neuropathologic outcome in rat brain following middle cerebral artery occlusion. In: Stroke. 1990 ; Vol. 21, No. 12. pp. 1727-1733.
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