Abstract
Purpose: To investigate wound healing in rabbits after corneal photoablation with a 213 nm UV scanning solid state laser. Materials and Methods: We used a frequency quintupled Nd:YAG laser to photoablate the cornea of 32 rabbits (5 mm ablation zone, 6 D myopic correction). The contralateral eyes and the eyes of 3 untreated animals served as controls. Light and electron microscopy analyses were performed on postoperative days 0, 7, 28, 90. Results: Abnormal, light basal epithelial cells were observed during the first postoperative month, but normal epithelial maturation was evident at 3 months. Basement membrane duplication was noted. Keratocyte proliferation occurred in the anterior stroma and in a few cases, in the posterior stroma, where keratocyte activation was present. Degenerative endothelial changes were observed immediately after ablation with intracellular disorganization and junction alteration. Surface profiles with variable regularity led to variable wound healing. Conclusions: Apart from differences in epithelial basal cell appearance, photoablation with a 213 nm solid state laser induced wound healing processes similar to those observed previously with prototype ArF excimer lasers. It is hoped that laser refinements will improve the surface regularity and lead to more consistent wound healing responses. However, several studies are required to assess mutagenicity, penetration depth and ablation rate of the 213nm wavelength on ocular tissues, as well as the effects of hydration on the clinical outcome.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 61-68 |
| Number of pages | 8 |
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 2393 |
| DOIs | |
| State | Published - May 22 1995 |
| Event | Ophthalmic Technologies V 1995 - San Jose, United States Duration: Feb 1 1995 → Feb 28 1995 |
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Keywords
- Cornea
- Histology
- Laser
- Photoablation
- Solid state lasers
- Tissue
- Wound healing
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering
Cite this
Corneal wound healing after photoablation with a 213nm scanning solid-state laser. Histological and ultrastructural study. / Legeais, Jean Marc; Parel, Jean Marie; Savoldelli, Michèle; Drubaix, Isabelle; Söderberg, Per; Manns, Fabrice; Renard, Gilles; Pouliquen, Yves.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 2393, 22.05.1995, p. 61-68.Research output: Contribution to journal › Conference article
}
TY - JOUR
T1 - Corneal wound healing after photoablation with a 213nm scanning solid-state laser. Histological and ultrastructural study
AU - Legeais, Jean Marc
AU - Parel, Jean Marie
AU - Savoldelli, Michèle
AU - Drubaix, Isabelle
AU - Söderberg, Per
AU - Manns, Fabrice
AU - Renard, Gilles
AU - Pouliquen, Yves
PY - 1995/5/22
Y1 - 1995/5/22
N2 - Purpose: To investigate wound healing in rabbits after corneal photoablation with a 213 nm UV scanning solid state laser. Materials and Methods: We used a frequency quintupled Nd:YAG laser to photoablate the cornea of 32 rabbits (5 mm ablation zone, 6 D myopic correction). The contralateral eyes and the eyes of 3 untreated animals served as controls. Light and electron microscopy analyses were performed on postoperative days 0, 7, 28, 90. Results: Abnormal, light basal epithelial cells were observed during the first postoperative month, but normal epithelial maturation was evident at 3 months. Basement membrane duplication was noted. Keratocyte proliferation occurred in the anterior stroma and in a few cases, in the posterior stroma, where keratocyte activation was present. Degenerative endothelial changes were observed immediately after ablation with intracellular disorganization and junction alteration. Surface profiles with variable regularity led to variable wound healing. Conclusions: Apart from differences in epithelial basal cell appearance, photoablation with a 213 nm solid state laser induced wound healing processes similar to those observed previously with prototype ArF excimer lasers. It is hoped that laser refinements will improve the surface regularity and lead to more consistent wound healing responses. However, several studies are required to assess mutagenicity, penetration depth and ablation rate of the 213nm wavelength on ocular tissues, as well as the effects of hydration on the clinical outcome.
AB - Purpose: To investigate wound healing in rabbits after corneal photoablation with a 213 nm UV scanning solid state laser. Materials and Methods: We used a frequency quintupled Nd:YAG laser to photoablate the cornea of 32 rabbits (5 mm ablation zone, 6 D myopic correction). The contralateral eyes and the eyes of 3 untreated animals served as controls. Light and electron microscopy analyses were performed on postoperative days 0, 7, 28, 90. Results: Abnormal, light basal epithelial cells were observed during the first postoperative month, but normal epithelial maturation was evident at 3 months. Basement membrane duplication was noted. Keratocyte proliferation occurred in the anterior stroma and in a few cases, in the posterior stroma, where keratocyte activation was present. Degenerative endothelial changes were observed immediately after ablation with intracellular disorganization and junction alteration. Surface profiles with variable regularity led to variable wound healing. Conclusions: Apart from differences in epithelial basal cell appearance, photoablation with a 213 nm solid state laser induced wound healing processes similar to those observed previously with prototype ArF excimer lasers. It is hoped that laser refinements will improve the surface regularity and lead to more consistent wound healing responses. However, several studies are required to assess mutagenicity, penetration depth and ablation rate of the 213nm wavelength on ocular tissues, as well as the effects of hydration on the clinical outcome.
KW - Cornea
KW - Histology
KW - Laser
KW - Photoablation
KW - Solid state lasers
KW - Tissue
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=85076792407&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85076792407&partnerID=8YFLogxK
U2 - 10.1117/12.209857
DO - 10.1117/12.209857
M3 - Conference article
AN - SCOPUS:85076792407
VL - 2393
SP - 61
EP - 68
JO - Proceedings of SPIE - The International Society for Optical Engineering
JF - Proceedings of SPIE - The International Society for Optical Engineering
SN - 0277-786X
ER -