Corneal stroma regeneration in felines after supradescemetic keratoprosthesis implantation

Ana C. Acosta, Edgar M. Espana, Josef Stoiber, Peggy D. Lamar, Fabiana Marangon, Eduardo C Alfonso, Jean-Marie A Parel

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

PURPOSE: To show corneal regeneration in 3 cats that underwent lamellar keratectomy (90%) depth during supradescemetic keratoprosthetic implantation. METHODS: Three 2-year-old cats that underwent spontaneous keratoprosthesis extrusion between 15 and 150 days after implanting a supradescemetic prosthesis into their right eyes were studied. Corneal structures and stroma thickness were evaluated by slit-lamp photographs, pachymetry, and confocal microscopy. Regenerated corneal epithelial cells, stroma matrix, and keratocyte morphology were studied with histology and transmission electron microscopy. Epithelial and stromal cell immunocharacterization was performed. RESULTS: Corneas progressively regained normal thickness and improved clarity within 40 to 60 days. Slit-lamp photographs and pachymetry showed gains in stromal thickness until 600 μm or more. In vivo confocal microscopy showed the restoration of normal epithelium and stroma in all cats. Corneal nerves were seen in the regenerated stroma of 2 cats. Immunostaining showed absent α-smooth muscle actin (SMA) expression and a keratin K3-expressing epithelium. Electron microscopy showed regeneration of normal epithelium with a well-formed basement membrane, organized corneal lamellae, and the presence of normal keratocytes. CONCLUSION: Felines are capable of regenerating corneal structures including epithelium and reinnervated stroma matrix after deep lamellar keratectomy. The use of feline models in corneal keratoprosthesis is therefore questionable.

Original languageEnglish
Pages (from-to)830-838
Number of pages9
JournalCornea
Volume25
Issue number7
DOIs
StatePublished - Aug 1 2006

Fingerprint

Corneal Stroma
Felidae
Regeneration
Cats
Epithelium
Confocal Microscopy
Epithelial Cells
Stromal Cells
Keratins
Transmission Electron Microscopy
Basement Membrane
Cornea
Prostheses and Implants
Smooth Muscle
Actins
Histology
Electron Microscopy
Slit Lamp

Keywords

  • Epithelium
  • Feline
  • Keratocytes
  • Keratoprosthesis
  • Regeneration

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Corneal stroma regeneration in felines after supradescemetic keratoprosthesis implantation. / Acosta, Ana C.; Espana, Edgar M.; Stoiber, Josef; Lamar, Peggy D.; Marangon, Fabiana; Alfonso, Eduardo C; Parel, Jean-Marie A.

In: Cornea, Vol. 25, No. 7, 01.08.2006, p. 830-838.

Research output: Contribution to journalArticle

Acosta, Ana C. ; Espana, Edgar M. ; Stoiber, Josef ; Lamar, Peggy D. ; Marangon, Fabiana ; Alfonso, Eduardo C ; Parel, Jean-Marie A. / Corneal stroma regeneration in felines after supradescemetic keratoprosthesis implantation. In: Cornea. 2006 ; Vol. 25, No. 7. pp. 830-838.
@article{af27fe902af94827a19c13522098e14b,
title = "Corneal stroma regeneration in felines after supradescemetic keratoprosthesis implantation",
abstract = "PURPOSE: To show corneal regeneration in 3 cats that underwent lamellar keratectomy (90{\%}) depth during supradescemetic keratoprosthetic implantation. METHODS: Three 2-year-old cats that underwent spontaneous keratoprosthesis extrusion between 15 and 150 days after implanting a supradescemetic prosthesis into their right eyes were studied. Corneal structures and stroma thickness were evaluated by slit-lamp photographs, pachymetry, and confocal microscopy. Regenerated corneal epithelial cells, stroma matrix, and keratocyte morphology were studied with histology and transmission electron microscopy. Epithelial and stromal cell immunocharacterization was performed. RESULTS: Corneas progressively regained normal thickness and improved clarity within 40 to 60 days. Slit-lamp photographs and pachymetry showed gains in stromal thickness until 600 μm or more. In vivo confocal microscopy showed the restoration of normal epithelium and stroma in all cats. Corneal nerves were seen in the regenerated stroma of 2 cats. Immunostaining showed absent α-smooth muscle actin (SMA) expression and a keratin K3-expressing epithelium. Electron microscopy showed regeneration of normal epithelium with a well-formed basement membrane, organized corneal lamellae, and the presence of normal keratocytes. CONCLUSION: Felines are capable of regenerating corneal structures including epithelium and reinnervated stroma matrix after deep lamellar keratectomy. The use of feline models in corneal keratoprosthesis is therefore questionable.",
keywords = "Epithelium, Feline, Keratocytes, Keratoprosthesis, Regeneration",
author = "Acosta, {Ana C.} and Espana, {Edgar M.} and Josef Stoiber and Lamar, {Peggy D.} and Fabiana Marangon and Alfonso, {Eduardo C} and Parel, {Jean-Marie A}",
year = "2006",
month = "8",
day = "1",
doi = "10.1097/01.ico.0000220769.19402.86",
language = "English",
volume = "25",
pages = "830--838",
journal = "Cornea",
issn = "0277-3740",
publisher = "Lippincott Williams and Wilkins",
number = "7",

}

TY - JOUR

T1 - Corneal stroma regeneration in felines after supradescemetic keratoprosthesis implantation

AU - Acosta, Ana C.

AU - Espana, Edgar M.

AU - Stoiber, Josef

AU - Lamar, Peggy D.

AU - Marangon, Fabiana

AU - Alfonso, Eduardo C

AU - Parel, Jean-Marie A

PY - 2006/8/1

Y1 - 2006/8/1

N2 - PURPOSE: To show corneal regeneration in 3 cats that underwent lamellar keratectomy (90%) depth during supradescemetic keratoprosthetic implantation. METHODS: Three 2-year-old cats that underwent spontaneous keratoprosthesis extrusion between 15 and 150 days after implanting a supradescemetic prosthesis into their right eyes were studied. Corneal structures and stroma thickness were evaluated by slit-lamp photographs, pachymetry, and confocal microscopy. Regenerated corneal epithelial cells, stroma matrix, and keratocyte morphology were studied with histology and transmission electron microscopy. Epithelial and stromal cell immunocharacterization was performed. RESULTS: Corneas progressively regained normal thickness and improved clarity within 40 to 60 days. Slit-lamp photographs and pachymetry showed gains in stromal thickness until 600 μm or more. In vivo confocal microscopy showed the restoration of normal epithelium and stroma in all cats. Corneal nerves were seen in the regenerated stroma of 2 cats. Immunostaining showed absent α-smooth muscle actin (SMA) expression and a keratin K3-expressing epithelium. Electron microscopy showed regeneration of normal epithelium with a well-formed basement membrane, organized corneal lamellae, and the presence of normal keratocytes. CONCLUSION: Felines are capable of regenerating corneal structures including epithelium and reinnervated stroma matrix after deep lamellar keratectomy. The use of feline models in corneal keratoprosthesis is therefore questionable.

AB - PURPOSE: To show corneal regeneration in 3 cats that underwent lamellar keratectomy (90%) depth during supradescemetic keratoprosthetic implantation. METHODS: Three 2-year-old cats that underwent spontaneous keratoprosthesis extrusion between 15 and 150 days after implanting a supradescemetic prosthesis into their right eyes were studied. Corneal structures and stroma thickness were evaluated by slit-lamp photographs, pachymetry, and confocal microscopy. Regenerated corneal epithelial cells, stroma matrix, and keratocyte morphology were studied with histology and transmission electron microscopy. Epithelial and stromal cell immunocharacterization was performed. RESULTS: Corneas progressively regained normal thickness and improved clarity within 40 to 60 days. Slit-lamp photographs and pachymetry showed gains in stromal thickness until 600 μm or more. In vivo confocal microscopy showed the restoration of normal epithelium and stroma in all cats. Corneal nerves were seen in the regenerated stroma of 2 cats. Immunostaining showed absent α-smooth muscle actin (SMA) expression and a keratin K3-expressing epithelium. Electron microscopy showed regeneration of normal epithelium with a well-formed basement membrane, organized corneal lamellae, and the presence of normal keratocytes. CONCLUSION: Felines are capable of regenerating corneal structures including epithelium and reinnervated stroma matrix after deep lamellar keratectomy. The use of feline models in corneal keratoprosthesis is therefore questionable.

KW - Epithelium

KW - Feline

KW - Keratocytes

KW - Keratoprosthesis

KW - Regeneration

UR - http://www.scopus.com/inward/record.url?scp=33750439147&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750439147&partnerID=8YFLogxK

U2 - 10.1097/01.ico.0000220769.19402.86

DO - 10.1097/01.ico.0000220769.19402.86

M3 - Article

VL - 25

SP - 830

EP - 838

JO - Cornea

JF - Cornea

SN - 0277-3740

IS - 7

ER -