Purpose. To determine if preserved hi man amniotic membrane can reduce corneal haze induced by Excimer laser photoabl ition. Methods. Excimer photoablation was performed bilaterally on 42 New Zealaid white rabbits with a 6 mm ablation zone and 120 |im depth (PTK mode) using tb; VISX Star. One eye was randomly covered by preserved human amniotic membnne secured with 4 interrupted 10-O nylon sutures, and the other eye served as the t ontroi. Amniotic membrane was removed at one week, and comeal haze was graded with a slit-lamp biomicroscope by three masked corneal specialists (WC, KH, and RKF) for the ensuing 12 weeks. Histology and in situ TUNEL staining (for fragn ented DNA as an index for apoptosis) was performed at 1, 3, and 7 days and 12 weeks. Results. At one week after photoablation, the amniotic membrane-c Dvered corneas showed more anterior stromal edema which resolved at 2nd week. A consistent grading of organized reticular comeal haze was noted among the three .nasked observers. Such corneal haze peaked at 7th week in both groups. The aniniotic membrane-covered group showed a statistically significant less corneal hazt (0.5 ±0.15) than the control group (1.25 ± 0.35) (p<0.001). Amniotic membrare covered corneas had less inflammatory response at 1 to 3 days, nearly nil DNA fragmentation on keratocytes on the ablated anterior stroma, less epithelial hyperplasia at 1 week, and less stromal fibroblast activation. Conclusion. Amniotic membrane matrix is effective in reducing corneal haze induced by Excimer photoablation n rabbits and may have clinical applications. Supported by NIH, NEI EY06819 (SCOT) and EY10900, Research to Prevent Blindness and a grant from Mr. & Mrs. Gardiner.
|Original language||English (US)|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - 1997|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience