Corneal haze induced by excimer laser photoablation in rabbit is reduced by preserved human amniotic membrane graft

Ming X. Wang, Trevor Gray, Pinnita Prabhasawat, Xiong Ma, William W Culbertson, Richard Forster, Khalil Hanna, Scheffer Tseng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We conducted a study to determine if preserved human amniotic membrane can reduce corneal haze induced by excimer laser photoablation, Excimer photoablation was performed bilaterally on 40 New Zealand white rabbits with a 6 mm ablation zone and 120 um depth (PTK) using the VISX Star. One eye was randomly covered with a preserved human amniotic membrane and secured using four interrupted 10-0) nylon sutures; the other eye served as control. The amniotic membranes were removed at one week, and the corneal haze was graded with a slit-lamp biomicroscopy by three masked corneal specialists (WC, KH and RF) biweekly for the ensuing 12 weeks. Histology and in situ TUNEL staining (for fragmented DNA as an index for apoptosis) was performed at days 1, 3 and 7 and at 12 weeks. One week after excimer photoablation, the amniotic membrane-covered corneas showed more anterior stromal edema, which resolved at the second week. A consistent grading of organized reticular corneal haze was noted among the three masked observers. Such corneal haze peaked at the seventh week in both groups. The amniotic membrane-covered group showed statistically significant less corneal haze (0.50 ± 0.15) than the control groups (1.25 ± 0.35) (p < 0.001). The amniotic membrane-covered corneas had less inflammatory response at days 1 and 3, showing nearly nil DNA fragmentation on keratocytes on the ablated anterior stromal and less stromal fibroblast activation. There is less altered epithelial cell morphology and less epithelial hyperplasia at 1 week in these amniotic membrane-treated eyes. We concluded from this study that amniotic membrane matrix is effective in reducing corneal haze induced by excimer photoablation in rabbits and may have clinical applications.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsP.O. Rol, K.M. Joos, F. Manns, A. Katzir
Pages109-119
Number of pages11
Volume3246
DOIs
StatePublished - 1998
Externally publishedYes
EventProceedings of Ophthalmic Technologies VIII - San Jose, CA, United States
Duration: Jan 24 1998Jan 25 1998

Other

OtherProceedings of Ophthalmic Technologies VIII
CountryUnited States
CitySan Jose, CA
Period1/24/981/25/98

Fingerprint

haze
rabbits
Excimer lasers
Grafts
excimer lasers
membranes
Membranes
excimers
cornea
DNA
deoxyribonucleic acid
edema
Histology
histology
Nylon (trademark)
New Zealand
fibroblasts
apoptosis
staining
Cell death

Keywords

  • Amniotic membrane transplantation
  • Apoptosis
  • Corneal haze
  • Corneal wound healing
  • Excimer laser
  • Extracellular matrix
  • Photorefractive keratectomy
  • Phototherapeutic keratectomy
  • Rabbit

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Wang, M. X., Gray, T., Prabhasawat, P., Ma, X., Culbertson, W. W., Forster, R., ... Tseng, S. (1998). Corneal haze induced by excimer laser photoablation in rabbit is reduced by preserved human amniotic membrane graft. In P. O. Rol, K. M. Joos, F. Manns, & A. Katzir (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3246, pp. 109-119) https://doi.org/10.1117/12.309422

Corneal haze induced by excimer laser photoablation in rabbit is reduced by preserved human amniotic membrane graft. / Wang, Ming X.; Gray, Trevor; Prabhasawat, Pinnita; Ma, Xiong; Culbertson, William W; Forster, Richard; Hanna, Khalil; Tseng, Scheffer.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / P.O. Rol; K.M. Joos; F. Manns; A. Katzir. Vol. 3246 1998. p. 109-119.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, MX, Gray, T, Prabhasawat, P, Ma, X, Culbertson, WW, Forster, R, Hanna, K & Tseng, S 1998, Corneal haze induced by excimer laser photoablation in rabbit is reduced by preserved human amniotic membrane graft. in PO Rol, KM Joos, F Manns & A Katzir (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 3246, pp. 109-119, Proceedings of Ophthalmic Technologies VIII, San Jose, CA, United States, 1/24/98. https://doi.org/10.1117/12.309422
Wang MX, Gray T, Prabhasawat P, Ma X, Culbertson WW, Forster R et al. Corneal haze induced by excimer laser photoablation in rabbit is reduced by preserved human amniotic membrane graft. In Rol PO, Joos KM, Manns F, Katzir A, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3246. 1998. p. 109-119 https://doi.org/10.1117/12.309422
Wang, Ming X. ; Gray, Trevor ; Prabhasawat, Pinnita ; Ma, Xiong ; Culbertson, William W ; Forster, Richard ; Hanna, Khalil ; Tseng, Scheffer. / Corneal haze induced by excimer laser photoablation in rabbit is reduced by preserved human amniotic membrane graft. Proceedings of SPIE - The International Society for Optical Engineering. editor / P.O. Rol ; K.M. Joos ; F. Manns ; A. Katzir. Vol. 3246 1998. pp. 109-119
@inproceedings{4ab1a38dc5284559b467f0add81de4bd,
title = "Corneal haze induced by excimer laser photoablation in rabbit is reduced by preserved human amniotic membrane graft",
abstract = "We conducted a study to determine if preserved human amniotic membrane can reduce corneal haze induced by excimer laser photoablation, Excimer photoablation was performed bilaterally on 40 New Zealand white rabbits with a 6 mm ablation zone and 120 um depth (PTK) using the VISX Star. One eye was randomly covered with a preserved human amniotic membrane and secured using four interrupted 10-0) nylon sutures; the other eye served as control. The amniotic membranes were removed at one week, and the corneal haze was graded with a slit-lamp biomicroscopy by three masked corneal specialists (WC, KH and RF) biweekly for the ensuing 12 weeks. Histology and in situ TUNEL staining (for fragmented DNA as an index for apoptosis) was performed at days 1, 3 and 7 and at 12 weeks. One week after excimer photoablation, the amniotic membrane-covered corneas showed more anterior stromal edema, which resolved at the second week. A consistent grading of organized reticular corneal haze was noted among the three masked observers. Such corneal haze peaked at the seventh week in both groups. The amniotic membrane-covered group showed statistically significant less corneal haze (0.50 ± 0.15) than the control groups (1.25 ± 0.35) (p < 0.001). The amniotic membrane-covered corneas had less inflammatory response at days 1 and 3, showing nearly nil DNA fragmentation on keratocytes on the ablated anterior stromal and less stromal fibroblast activation. There is less altered epithelial cell morphology and less epithelial hyperplasia at 1 week in these amniotic membrane-treated eyes. We concluded from this study that amniotic membrane matrix is effective in reducing corneal haze induced by excimer photoablation in rabbits and may have clinical applications.",
keywords = "Amniotic membrane transplantation, Apoptosis, Corneal haze, Corneal wound healing, Excimer laser, Extracellular matrix, Photorefractive keratectomy, Phototherapeutic keratectomy, Rabbit",
author = "Wang, {Ming X.} and Trevor Gray and Pinnita Prabhasawat and Xiong Ma and Culbertson, {William W} and Richard Forster and Khalil Hanna and Scheffer Tseng",
year = "1998",
doi = "10.1117/12.309422",
language = "English (US)",
volume = "3246",
pages = "109--119",
editor = "P.O. Rol and K.M. Joos and F. Manns and A. Katzir",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

}

TY - GEN

T1 - Corneal haze induced by excimer laser photoablation in rabbit is reduced by preserved human amniotic membrane graft

AU - Wang, Ming X.

AU - Gray, Trevor

AU - Prabhasawat, Pinnita

AU - Ma, Xiong

AU - Culbertson, William W

AU - Forster, Richard

AU - Hanna, Khalil

AU - Tseng, Scheffer

PY - 1998

Y1 - 1998

N2 - We conducted a study to determine if preserved human amniotic membrane can reduce corneal haze induced by excimer laser photoablation, Excimer photoablation was performed bilaterally on 40 New Zealand white rabbits with a 6 mm ablation zone and 120 um depth (PTK) using the VISX Star. One eye was randomly covered with a preserved human amniotic membrane and secured using four interrupted 10-0) nylon sutures; the other eye served as control. The amniotic membranes were removed at one week, and the corneal haze was graded with a slit-lamp biomicroscopy by three masked corneal specialists (WC, KH and RF) biweekly for the ensuing 12 weeks. Histology and in situ TUNEL staining (for fragmented DNA as an index for apoptosis) was performed at days 1, 3 and 7 and at 12 weeks. One week after excimer photoablation, the amniotic membrane-covered corneas showed more anterior stromal edema, which resolved at the second week. A consistent grading of organized reticular corneal haze was noted among the three masked observers. Such corneal haze peaked at the seventh week in both groups. The amniotic membrane-covered group showed statistically significant less corneal haze (0.50 ± 0.15) than the control groups (1.25 ± 0.35) (p < 0.001). The amniotic membrane-covered corneas had less inflammatory response at days 1 and 3, showing nearly nil DNA fragmentation on keratocytes on the ablated anterior stromal and less stromal fibroblast activation. There is less altered epithelial cell morphology and less epithelial hyperplasia at 1 week in these amniotic membrane-treated eyes. We concluded from this study that amniotic membrane matrix is effective in reducing corneal haze induced by excimer photoablation in rabbits and may have clinical applications.

AB - We conducted a study to determine if preserved human amniotic membrane can reduce corneal haze induced by excimer laser photoablation, Excimer photoablation was performed bilaterally on 40 New Zealand white rabbits with a 6 mm ablation zone and 120 um depth (PTK) using the VISX Star. One eye was randomly covered with a preserved human amniotic membrane and secured using four interrupted 10-0) nylon sutures; the other eye served as control. The amniotic membranes were removed at one week, and the corneal haze was graded with a slit-lamp biomicroscopy by three masked corneal specialists (WC, KH and RF) biweekly for the ensuing 12 weeks. Histology and in situ TUNEL staining (for fragmented DNA as an index for apoptosis) was performed at days 1, 3 and 7 and at 12 weeks. One week after excimer photoablation, the amniotic membrane-covered corneas showed more anterior stromal edema, which resolved at the second week. A consistent grading of organized reticular corneal haze was noted among the three masked observers. Such corneal haze peaked at the seventh week in both groups. The amniotic membrane-covered group showed statistically significant less corneal haze (0.50 ± 0.15) than the control groups (1.25 ± 0.35) (p < 0.001). The amniotic membrane-covered corneas had less inflammatory response at days 1 and 3, showing nearly nil DNA fragmentation on keratocytes on the ablated anterior stromal and less stromal fibroblast activation. There is less altered epithelial cell morphology and less epithelial hyperplasia at 1 week in these amniotic membrane-treated eyes. We concluded from this study that amniotic membrane matrix is effective in reducing corneal haze induced by excimer photoablation in rabbits and may have clinical applications.

KW - Amniotic membrane transplantation

KW - Apoptosis

KW - Corneal haze

KW - Corneal wound healing

KW - Excimer laser

KW - Extracellular matrix

KW - Photorefractive keratectomy

KW - Phototherapeutic keratectomy

KW - Rabbit

UR - http://www.scopus.com/inward/record.url?scp=0032225180&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032225180&partnerID=8YFLogxK

U2 - 10.1117/12.309422

DO - 10.1117/12.309422

M3 - Conference contribution

AN - SCOPUS:0032225180

VL - 3246

SP - 109

EP - 119

BT - Proceedings of SPIE - The International Society for Optical Engineering

A2 - Rol, P.O.

A2 - Joos, K.M.

A2 - Manns, F.

A2 - Katzir, A.

ER -