Copper uptake across rainbow trout gills: Mechanisms of apical entry

Martin Grosell, Chris M. Wood

Research output: Contribution to journalArticle

217 Citations (Scopus)

Abstract

Several components of branchial copper uptake were identified in juvenile freshwater rainbow trout (Oncorhynchus mykiss) using 64Cu. On the basis of competitive interactions between sodium and copper uptake, inhibition of copper uptake by a proton pump inhibitor (bafilomycin A1, 2 μmol l-1) and a Na+ channel blocker (phenamil, 100 μmol l-1), it appears that a proportion of the branchial copper uptake occurs via an apical Na+ channel. This sodium-sensitive copper uptake demonstrates saturation kinetics, with a Km of 7.1 nmol l-1 and a Jmax of 21.2 pmol g-1 h-l, and is characterized by an IC50 of 104 μmol l-1 sodium. On the basis of residual copper uptake in the presence of high sodium concentrations (20 mmol l-1) and differential inhibition of sodium and copper uptake by phenamil (100 μmol l-1), a sodium-insensitive component of copper uptake is also present in trout gills. It demonstrates saturation kinetics with a comparably low Km (9.6 nmol l-1) but a lower maximum transport capacity (Jmax=3.5 pmol g-1 h-1) than the sodium-insensitive system. Sodium uptake exhibits saturation kinetics with a Km of 69 μmol l-1. Copper reduced branchial sodium transport affinity but increased the maximal sodium transport capacity.

Original languageEnglish
Pages (from-to)1179-1188
Number of pages10
JournalJournal of Experimental Biology
Volume205
Issue number8
StatePublished - Aug 19 2002
Externally publishedYes

Fingerprint

Oncorhynchus mykiss
rainbow
Copper
gills
copper
Sodium
sodium
uptake mechanisms
saturation
kinetics
Trout
Proton Pump Inhibitors
Fresh Water
trout
Inhibitory Concentration 50
inhibitory concentration 50
inhibitor
pump

Keywords

  • Copper
  • Copper/sodium interactions
  • Gill
  • Homeostasis
  • Oncorhynchus mykiss
  • Rainbow trout
  • Sodium uptake

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Copper uptake across rainbow trout gills : Mechanisms of apical entry. / Grosell, Martin; Wood, Chris M.

In: Journal of Experimental Biology, Vol. 205, No. 8, 19.08.2002, p. 1179-1188.

Research output: Contribution to journalArticle

@article{919b5ed7aa664ce7a59e8d33a64c74e7,
title = "Copper uptake across rainbow trout gills: Mechanisms of apical entry",
abstract = "Several components of branchial copper uptake were identified in juvenile freshwater rainbow trout (Oncorhynchus mykiss) using 64Cu. On the basis of competitive interactions between sodium and copper uptake, inhibition of copper uptake by a proton pump inhibitor (bafilomycin A1, 2 μmol l-1) and a Na+ channel blocker (phenamil, 100 μmol l-1), it appears that a proportion of the branchial copper uptake occurs via an apical Na+ channel. This sodium-sensitive copper uptake demonstrates saturation kinetics, with a Km of 7.1 nmol l-1 and a Jmax of 21.2 pmol g-1 h-l, and is characterized by an IC50 of 104 μmol l-1 sodium. On the basis of residual copper uptake in the presence of high sodium concentrations (20 mmol l-1) and differential inhibition of sodium and copper uptake by phenamil (100 μmol l-1), a sodium-insensitive component of copper uptake is also present in trout gills. It demonstrates saturation kinetics with a comparably low Km (9.6 nmol l-1) but a lower maximum transport capacity (Jmax=3.5 pmol g-1 h-1) than the sodium-insensitive system. Sodium uptake exhibits saturation kinetics with a Km of 69 μmol l-1. Copper reduced branchial sodium transport affinity but increased the maximal sodium transport capacity.",
keywords = "Copper, Copper/sodium interactions, Gill, Homeostasis, Oncorhynchus mykiss, Rainbow trout, Sodium uptake",
author = "Martin Grosell and Wood, {Chris M.}",
year = "2002",
month = "8",
day = "19",
language = "English",
volume = "205",
pages = "1179--1188",
journal = "Journal of Experimental Biology",
issn = "0022-0949",
publisher = "Company of Biologists Ltd",
number = "8",

}

TY - JOUR

T1 - Copper uptake across rainbow trout gills

T2 - Mechanisms of apical entry

AU - Grosell, Martin

AU - Wood, Chris M.

PY - 2002/8/19

Y1 - 2002/8/19

N2 - Several components of branchial copper uptake were identified in juvenile freshwater rainbow trout (Oncorhynchus mykiss) using 64Cu. On the basis of competitive interactions between sodium and copper uptake, inhibition of copper uptake by a proton pump inhibitor (bafilomycin A1, 2 μmol l-1) and a Na+ channel blocker (phenamil, 100 μmol l-1), it appears that a proportion of the branchial copper uptake occurs via an apical Na+ channel. This sodium-sensitive copper uptake demonstrates saturation kinetics, with a Km of 7.1 nmol l-1 and a Jmax of 21.2 pmol g-1 h-l, and is characterized by an IC50 of 104 μmol l-1 sodium. On the basis of residual copper uptake in the presence of high sodium concentrations (20 mmol l-1) and differential inhibition of sodium and copper uptake by phenamil (100 μmol l-1), a sodium-insensitive component of copper uptake is also present in trout gills. It demonstrates saturation kinetics with a comparably low Km (9.6 nmol l-1) but a lower maximum transport capacity (Jmax=3.5 pmol g-1 h-1) than the sodium-insensitive system. Sodium uptake exhibits saturation kinetics with a Km of 69 μmol l-1. Copper reduced branchial sodium transport affinity but increased the maximal sodium transport capacity.

AB - Several components of branchial copper uptake were identified in juvenile freshwater rainbow trout (Oncorhynchus mykiss) using 64Cu. On the basis of competitive interactions between sodium and copper uptake, inhibition of copper uptake by a proton pump inhibitor (bafilomycin A1, 2 μmol l-1) and a Na+ channel blocker (phenamil, 100 μmol l-1), it appears that a proportion of the branchial copper uptake occurs via an apical Na+ channel. This sodium-sensitive copper uptake demonstrates saturation kinetics, with a Km of 7.1 nmol l-1 and a Jmax of 21.2 pmol g-1 h-l, and is characterized by an IC50 of 104 μmol l-1 sodium. On the basis of residual copper uptake in the presence of high sodium concentrations (20 mmol l-1) and differential inhibition of sodium and copper uptake by phenamil (100 μmol l-1), a sodium-insensitive component of copper uptake is also present in trout gills. It demonstrates saturation kinetics with a comparably low Km (9.6 nmol l-1) but a lower maximum transport capacity (Jmax=3.5 pmol g-1 h-1) than the sodium-insensitive system. Sodium uptake exhibits saturation kinetics with a Km of 69 μmol l-1. Copper reduced branchial sodium transport affinity but increased the maximal sodium transport capacity.

KW - Copper

KW - Copper/sodium interactions

KW - Gill

KW - Homeostasis

KW - Oncorhynchus mykiss

KW - Rainbow trout

KW - Sodium uptake

UR - http://www.scopus.com/inward/record.url?scp=0036330480&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036330480&partnerID=8YFLogxK

M3 - Article

C2 - 11919277

AN - SCOPUS:0036330480

VL - 205

SP - 1179

EP - 1188

JO - Journal of Experimental Biology

JF - Journal of Experimental Biology

SN - 0022-0949

IS - 8

ER -