Copper binding dynamics and olfactory impairment in fathead minnows (Pimephales promelas)

Warren W. Green, Reehan S. Mirza, Chrism Wood, Greg G. Pyle

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

When fish are exposed to sublethal, environmentally relevant Cu concentrations, olfactory acuity is impaired. The goals of the present study were to investigate the binding dynamics of waterborne Cu in the olfactory epithelium (OE), to examine the influence of calcium (Ca2+) on Cu binding, and to link Cu-OE binding to changes in olfactory acuity. Using short-term in vivo waterborne exposures to 64Cu, we found that Cu accumulates rapidly in the OE, reaching a plateau by 3 h. The binding affinity (log KCu-OE) and binding capacity (Bmax) of 64Cu in the OE were 6.7 and 10.0 nmol Cu g-1, respectively. As waterborne Ca2+ was increased from 50 to 1000 μM L-1, the Bmax of Cu decreased by ~50% while the log K Cu-OE remained constant, indicative of noncompetitive inhibition. Using electro-olfactograms (EOG), short-term exposures to 160 and 240 nmol Cu L-1 were found to reduce olfactory responses to 10-5 M L-arginine by 72 and 79%, respectively. Short-term exposure to 160 nmol Cu L-1 also caused a 15-fold reduction in behavioral responses toafood stimulus. Interestingly, increasing waterborne Ca2+ did not reduce the effects of Cu on EOG or behavioral responses. These results demonstrate that short-term, environmentally realistic concentrations of Cu not only bind to the OE of fathead minnows but also impair their olfactory sensitivity and behavioral responses to olfactory stimuli. Waterborne Ca2+ reduces Cu-OE binding but does not protect against olfactory impairment.

Original languageEnglish
Pages (from-to)1431-1437
Number of pages7
JournalEnvironmental Science and Technology
Volume44
Issue number4
DOIs
StatePublished - Mar 1 2010

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behavioral response
Fish
Arginine
Copper
copper
Calcium
calcium
plateau
fold
fish
exposure

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Copper binding dynamics and olfactory impairment in fathead minnows (Pimephales promelas). / Green, Warren W.; Mirza, Reehan S.; Wood, Chrism; Pyle, Greg G.

In: Environmental Science and Technology, Vol. 44, No. 4, 01.03.2010, p. 1431-1437.

Research output: Contribution to journalArticle

Green, Warren W. ; Mirza, Reehan S. ; Wood, Chrism ; Pyle, Greg G. / Copper binding dynamics and olfactory impairment in fathead minnows (Pimephales promelas). In: Environmental Science and Technology. 2010 ; Vol. 44, No. 4. pp. 1431-1437.
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