Coordinated regulation of ATF2 by miR-26b in γ-irradiated lung cancer cells

Himanshu Arora, Rehana Qureshi, Ae Kyung Park, Woong Yang Park

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

MicroRNA regulates cellular responses to ionizing radiation (IR) through translational control of target genes. We analyzed time-series changes in microRNA expression following γ-irradiation in H1299 lung cancer cells using microarray analysis. Significantly changed IR-responsive microRNAs were selected based on analysis of variance analysis, and predicted target mRNAs were enriched in mitogen-activated protein kinase (MAPK) signaling. Concurrent analysis of time-series mRNA and microRNA profiles uncovered that expression of miR-26b was down regulated, and its target activating transcription factor 2 (ATF2) mRNA was up regulated in γ-irradiated H1299 cells. IR in miR-26b overexpressed H1299 cells could not induce expression of ATF2. When c-Jun N-terminal kinase activity was inhibited using SP600125, expression of miR-26b was induced following γ-irradiation in H1299 cells. From these results, we concluded that IR-induced up-regulation of ATF2 was coordinately enhanced by suppression of miR-26b in lung cancer cells, which may enhance the effect of IR in the MAPK signaling pathway.

Original languageEnglish (US)
Article numbere23802
JournalPloS one
Volume6
Issue number8
DOIs
StatePublished - Aug 25 2011
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint

Dive into the research topics of 'Coordinated regulation of ATF2 by miR-26b in γ-irradiated lung cancer cells'. Together they form a unique fingerprint.

Cite this