Coordinate reciprocal trends in glycolytic and mitochondrial transcript accumulations during the in vitro differentiation of human myoblasts

Keith A Webster, Peter Gunning, Edna Hardeman, Douglas C. Wallace, Larry Kedes

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Changes in the mRNA levels during mammalian myogenesis were compared for seven polypeptides of mitochondrial respiration (the mitochondrial DNA-encoded cytochrome oxidase subunit III, ATP synthase subunit 6, NADH dehydrogenase subunits 1 and 2, and 16S ribosomal RNA; the nuclear encoded ATP synthase β subunit and the adenine nucleotide translocase) and three polypeptides of glycolysis (glyceraldehyde-3-phosphate dehydrogenase, pyruvate kinase, and triose-phosphate isomerase). Progressive changes during the conversion from myoblasts to myotubes were monitored under both atmospheric oxygen (normoxic) and hypoxic environments. Northern analyses revealed coordinate, biphasic, and reciprocal expression of the respiratory and glycolytic mRNAs during myogenesis. In normoxic cells the mitochondrial respiratory enzymes were highest in myoblasts, declined 3- to 5-fold during commitment and exit from the cell cycle, and increased progressively as the myotubes matured. By contrast, the glycolytic enzyme mRNAs rose 3- to 6-fold on commitment and then progressively declined. When partially differentiated myotubes were switched to hypoxic conditions, the glycolytic enzyme mRNAs increased and the respiratory mRNAs declined. Hence, the developmental regulation of muscle bioenergetic metabolism appears to be regulated at the pretranslational level and is modulated by oxygen tension.

Original languageEnglish
Pages (from-to)566-573
Number of pages8
JournalJournal of Cellular Physiology
Volume142
Issue number3
StatePublished - Mar 1 1990
Externally publishedYes

Fingerprint

Myoblasts
Skeletal Muscle Fibers
Messenger RNA
Muscle Development
Enzymes
ATP Translocases Mitochondrial ADP
Triose-Phosphate Isomerase
16S Ribosomal RNA
Oxygen
NADH Dehydrogenase
Peptides
Glyceraldehyde-3-Phosphate Dehydrogenases
Pyruvate Kinase
Glycolysis
Electron Transport Complex IV
Mitochondrial DNA
Metabolism
Energy Metabolism
Muscle
Cell Cycle

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Coordinate reciprocal trends in glycolytic and mitochondrial transcript accumulations during the in vitro differentiation of human myoblasts. / Webster, Keith A; Gunning, Peter; Hardeman, Edna; Wallace, Douglas C.; Kedes, Larry.

In: Journal of Cellular Physiology, Vol. 142, No. 3, 01.03.1990, p. 566-573.

Research output: Contribution to journalArticle

Webster, Keith A ; Gunning, Peter ; Hardeman, Edna ; Wallace, Douglas C. ; Kedes, Larry. / Coordinate reciprocal trends in glycolytic and mitochondrial transcript accumulations during the in vitro differentiation of human myoblasts. In: Journal of Cellular Physiology. 1990 ; Vol. 142, No. 3. pp. 566-573.
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