Coordinate estrogen-regulated instability of serum protein-coding messenger RNAs in Xenopus laevis

Ricardo L. Pastori, John E. Moskaitis, Susan W. Buzek, Daniel R. Schoenberg

Research output: Contribution to journalArticle

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Abstract

Estrogen causes the cytoplasmic destabilization of albumin and γ-fibrinogen mRNA in Xenopus laevis liver. The purpose of the present study was to determine whether mRNA destabilization is a generalized phenomenon in response to estrogen, or whether this process is restricted to a particular class of mRNAs. To address this, we have expanded our bank of serum protein-coding cDNA clones to include transferrin, the second protein of inter-α-trypsin inhibitor and clone 12B, for which there is no mammalian homolog. Together with albumin and γ-fibrinogen, these represent more than 85% of the mRNAs encoding liver secreted proteins. Estrogen administration to male Xenopus or to liver explant cultures causes the generalized disappearance of all of these mRNAs. In contrast, estrogen has no effect on actin, ferritin, or poly(A)-binding protein mRNA, all of which encode intracellular proteins. We have previously demonstrated that albumin mRNA is degraded in both messenger ribonucleoprotein and polysome fractions. Sucrose gradient analysis demonstrates the same pattern for degradation of all other serum protein-coding mRNAs. Estrogen has no effect on the amounts or gradient distribution of actin, ferritin, or poly(A)-binding protein mRNA. We conclude that regulated destabilization of mRNAs encoding secreted proteins is a generalized phenomenon in response to estrogen stimulation of Xenopus liver.

Original languageEnglish (US)
Pages (from-to)461-468
Number of pages8
JournalMolecular Endocrinology
Volume5
Issue number4
DOIs
StatePublished - Apr 1991

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ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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